Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF696 and GFF697 are separated by 207 nucleotides GFF697 and GFF698 are separated by 29 nucleotides
GFF696 - ATP-dependent RNA helicase RhlE, at 52,650 to 54,014
GFF696
GFF697 - ATP-dependent helicase DinG/Rad3, at 54,222 to 56,366
GFF697
GFF698 - Anthranilate phosphoribosyltransferase like (EC 2.4.2.18), at 56,396 to 57,370
GFF698
Position (kb) 54
55
56
57 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 53.523 kb on - strand, within GFF696 at 53.558 kb on + strand, within GFF696 at 53.607 kb on + strand, within GFF696 at 53.608 kb on - strand, within GFF696 at 53.634 kb on + strand, within GFF696 at 53.635 kb on - strand, within GFF696 at 53.635 kb on - strand, within GFF696 at 53.635 kb on - strand, within GFF696 at 53.635 kb on - strand, within GFF696 at 53.638 kb on + strand, within GFF696 at 53.711 kb on + strand, within GFF696 at 53.763 kb on - strand, within GFF696 at 53.906 kb on + strand at 54.074 kb on + strand at 54.074 kb on + strand at 54.075 kb on - strand at 54.075 kb on - strand at 54.151 kb on + strand at 54.266 kb on + strand at 54.296 kb on + strand at 54.411 kb on + strand at 54.573 kb on + strand, within GFF697 at 54.573 kb on + strand, within GFF697 at 54.574 kb on - strand, within GFF697 at 54.786 kb on - strand, within GFF697 at 54.823 kb on - strand, within GFF697 at 54.959 kb on - strand, within GFF697 at 55.154 kb on - strand, within GFF697 at 55.155 kb on + strand, within GFF697 at 55.187 kb on + strand, within GFF697 at 55.188 kb on - strand, within GFF697 at 55.188 kb on - strand, within GFF697 at 55.194 kb on + strand, within GFF697 at 55.194 kb on + strand, within GFF697 at 55.196 kb on + strand, within GFF697 at 55.197 kb on - strand, within GFF697 at 55.586 kb on + strand, within GFF697 at 55.697 kb on + strand, within GFF697 at 55.772 kb on + strand, within GFF697 at 55.829 kb on + strand, within GFF697 at 56.022 kb on - strand, within GFF697 at 56.321 kb on + strand at 56.322 kb on - strand at 56.480 kb on + strand at 56.480 kb on + strand at 56.528 kb on - strand, within GFF698 at 56.528 kb on - strand, within GFF698 at 56.528 kb on - strand, within GFF698 at 56.529 kb on + strand, within GFF698 at 56.529 kb on + strand, within GFF698 at 56.530 kb on - strand, within GFF698 at 56.582 kb on + strand, within GFF698 at 56.602 kb on + strand, within GFF698 at 56.734 kb on - strand, within GFF698 at 56.812 kb on - strand, within GFF698 at 56.812 kb on - strand, within GFF698 at 56.991 kb on + strand, within GFF698 at 57.150 kb on + strand, within GFF698 at 57.176 kb on + strand, within GFF698 at 57.327 kb on + strand at 57.327 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 53,523 - GFF696 0.64 -0.0 53,558 + GFF696 0.67 -0.7 53,607 + GFF696 0.70 -0.9 53,608 - GFF696 0.70 -0.2 53,634 + GFF696 0.72 -0.5 53,635 - GFF696 0.72 +0.9 53,635 - GFF696 0.72 +1.3 53,635 - GFF696 0.72 -0.1 53,635 - GFF696 0.72 -2.0 53,638 + GFF696 0.72 +0.6 53,711 + GFF696 0.78 -0.1 53,763 - GFF696 0.82 +0.6 53,906 + +0.3 54,074 + -1.3 54,074 + +0.4 54,075 - +0.6 54,075 - -0.5 54,151 + -0.2 54,266 + -0.1 54,296 + +0.9 54,411 + -0.5 54,573 + GFF697 0.16 -0.6 54,573 + GFF697 0.16 +2.1 54,574 - GFF697 0.16 +0.6 54,786 - GFF697 0.26 -0.4 54,823 - GFF697 0.28 -0.4 54,959 - GFF697 0.34 -1.3 55,154 - GFF697 0.43 -0.5 55,155 + GFF697 0.43 -0.6 55,187 + GFF697 0.45 +1.1 55,188 - GFF697 0.45 +0.0 55,188 - GFF697 0.45 -0.6 55,194 + GFF697 0.45 -1.4 55,194 + GFF697 0.45 -1.6 55,196 + GFF697 0.45 -0.1 55,197 - GFF697 0.45 -0.5 55,586 + GFF697 0.64 -2.9 55,697 + GFF697 0.69 -1.8 55,772 + GFF697 0.72 -4.0 55,829 + GFF697 0.75 -0.4 56,022 - GFF697 0.84 -1.1 56,321 + -0.8 56,322 - +0.5 56,480 + -0.6 56,480 + -0.8 56,528 - GFF698 0.14 +1.7 56,528 - GFF698 0.14 -0.0 56,528 - GFF698 0.14 -0.1 56,529 + GFF698 0.14 +0.2 56,529 + GFF698 0.14 -0.4 56,530 - GFF698 0.14 -1.2 56,582 + GFF698 0.19 +0.2 56,602 + GFF698 0.21 +0.3 56,734 - GFF698 0.35 -0.3 56,812 - GFF698 0.43 -0.4 56,812 - GFF698 0.43 -0.3 56,991 + GFF698 0.61 +1.3 57,150 + GFF698 0.77 -0.3 57,176 + GFF698 0.80 -0.1 57,327 + +0.0 57,327 + +0.2
Or see this region's nucleotide sequence