Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4887 and GFF4888 are separated by 90 nucleotides GFF4888 and GFF4889 are separated by 311 nucleotides GFF4889 and GFF4890 are separated by 44 nucleotides
GFF4887 - Transcriptional regulator, LysR family, at 3,667 to 4,578
GFF4887
GFF4888 - Translation initiation factor 1, at 4,669 to 4,887
GFF4888
GFF4889 - Leucyl/phenylalanyl-tRNA--protein transferase (EC 2.3.2.6), at 5,199 to 5,903
GFF4889
GFF4890 - Transport ATP-binding protein CydC, at 5,948 to 7,669
GFF4890
Position (kb) 5
6 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4.332 kb on + strand, within GFF4887 at 4.406 kb on + strand, within GFF4887 at 4.512 kb on - strand at 4.523 kb on + strand at 4.523 kb on + strand at 4.600 kb on + strand at 4.637 kb on - strand at 5.069 kb on + strand at 5.080 kb on + strand at 5.080 kb on + strand at 5.081 kb on - strand at 5.081 kb on - strand at 5.082 kb on + strand at 5.083 kb on - strand at 5.083 kb on - strand at 5.083 kb on - strand at 5.113 kb on - strand at 5.135 kb on - strand at 5.168 kb on - strand at 5.470 kb on + strand, within GFF4889 at 5.558 kb on + strand, within GFF4889 at 5.788 kb on - strand, within GFF4889 at 5.941 kb on + strand at 5.949 kb on + strand at 6.122 kb on + strand, within GFF4890 at 6.274 kb on + strand, within GFF4890 at 6.274 kb on + strand, within GFF4890 at 6.887 kb on + strand, within GFF4890 at 6.887 kb on + strand, within GFF4890 at 6.888 kb on - strand, within GFF4890 at 6.888 kb on - strand, within GFF4890
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 4,332 + GFF4887 0.73 -0.2 4,406 + GFF4887 0.81 +0.8 4,512 - -0.0 4,523 + -1.8 4,523 + +0.2 4,600 + +0.4 4,637 - -0.2 5,069 + -0.4 5,080 + -0.0 5,080 + -0.2 5,081 - +2.1 5,081 - -0.4 5,082 + -0.6 5,083 - -0.7 5,083 - -0.8 5,083 - -0.1 5,113 - +0.5 5,135 - +0.3 5,168 - -0.2 5,470 + GFF4889 0.38 +0.1 5,558 + GFF4889 0.51 +1.5 5,788 - GFF4889 0.84 +0.4 5,941 + -0.2 5,949 + +0.9 6,122 + GFF4890 0.10 +0.9 6,274 + GFF4890 0.19 -0.9 6,274 + GFF4890 0.19 -0.6 6,887 + GFF4890 0.55 -1.0 6,887 + GFF4890 0.55 -0.6 6,888 - GFF4890 0.55 +0.0 6,888 - GFF4890 0.55 +1.6
Or see this region's nucleotide sequence