Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF468 and GFF469 are separated by 5 nucleotides GFF469 and GFF470 are separated by 217 nucleotides GFF470 and GFF638 are separated by 90 nucleotides GFF638 and GFF471 are separated by 159 nucleotides
GFF468 - Putative fimbrial protein, at 188,981 to 190,060
GFF468
GFF469 - putative fimbrial protein precursor, at 190,066 to 190,593
GFF469
GFF470 - Phosphoethanolamine transferase specific for the outer Kdo residue of lipopolysaccharide, at 190,811 to 192,502
GFF470
GFF638 - tRNA-Pro-CGG, at 192,593 to 192,669
GFF638
GFF471 - Putative lacI-family transcriptional regulator, at 192,829 to 193,836
GFF471
Position (kb) 190
191
192
193 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 190.045 kb on + strand at 190.046 kb on - strand at 190.059 kb on - strand at 190.164 kb on + strand, within GFF469 at 190.174 kb on - strand, within GFF469 at 190.176 kb on + strand, within GFF469 at 190.209 kb on + strand, within GFF469 at 190.209 kb on + strand, within GFF469 at 190.439 kb on - strand, within GFF469 at 190.492 kb on - strand, within GFF469 at 190.508 kb on - strand, within GFF469 at 190.509 kb on + strand, within GFF469 at 190.519 kb on + strand, within GFF469 at 190.520 kb on - strand, within GFF469 at 190.586 kb on - strand at 190.698 kb on + strand at 190.702 kb on + strand at 190.703 kb on - strand at 190.724 kb on + strand at 190.724 kb on + strand at 190.725 kb on - strand at 190.725 kb on - strand at 190.744 kb on - strand at 190.748 kb on - strand at 190.748 kb on - strand at 190.748 kb on - strand at 190.865 kb on + strand at 190.866 kb on - strand at 190.916 kb on + strand at 190.917 kb on - strand at 190.996 kb on - strand, within GFF470 at 191.079 kb on - strand, within GFF470 at 191.181 kb on + strand, within GFF470 at 191.181 kb on + strand, within GFF470 at 191.182 kb on - strand, within GFF470 at 191.230 kb on + strand, within GFF470 at 191.244 kb on - strand, within GFF470 at 191.244 kb on - strand, within GFF470 at 191.244 kb on - strand, within GFF470 at 191.262 kb on + strand, within GFF470 at 191.262 kb on + strand, within GFF470 at 191.263 kb on - strand, within GFF470 at 191.266 kb on + strand, within GFF470 at 191.397 kb on - strand, within GFF470 at 191.420 kb on + strand, within GFF470 at 191.457 kb on - strand, within GFF470 at 191.525 kb on + strand, within GFF470 at 191.526 kb on - strand, within GFF470 at 191.555 kb on + strand, within GFF470 at 191.555 kb on + strand, within GFF470 at 191.613 kb on - strand, within GFF470 at 191.624 kb on - strand, within GFF470 at 191.738 kb on - strand, within GFF470 at 191.752 kb on + strand, within GFF470 at 191.753 kb on - strand, within GFF470 at 191.753 kb on - strand, within GFF470 at 191.758 kb on - strand, within GFF470 at 191.840 kb on + strand, within GFF470 at 191.841 kb on - strand, within GFF470 at 191.841 kb on - strand, within GFF470 at 191.863 kb on + strand, within GFF470 at 191.864 kb on - strand, within GFF470 at 191.870 kb on + strand, within GFF470 at 192.017 kb on + strand, within GFF470 at 192.065 kb on - strand, within GFF470 at 192.110 kb on + strand, within GFF470 at 192.111 kb on - strand, within GFF470 at 192.116 kb on - strand, within GFF470 at 192.128 kb on + strand, within GFF470 at 192.128 kb on + strand, within GFF470 at 192.128 kb on + strand, within GFF470 at 192.128 kb on + strand, within GFF470 at 192.128 kb on + strand, within GFF470 at 192.142 kb on + strand, within GFF470 at 192.305 kb on - strand, within GFF470 at 192.393 kb on + strand at 192.393 kb on + strand at 192.393 kb on + strand at 192.393 kb on + strand at 192.394 kb on - strand at 192.394 kb on - strand at 192.394 kb on - strand at 192.396 kb on - strand at 192.424 kb on + strand at 192.424 kb on + strand at 192.424 kb on + strand at 192.425 kb on - strand at 192.500 kb on + strand at 192.577 kb on - strand at 192.810 kb on - strand at 193.129 kb on + strand, within GFF471 at 193.130 kb on - strand, within GFF471 at 193.130 kb on - strand, within GFF471 at 193.130 kb on - strand, within GFF471 at 193.274 kb on - strand, within GFF471
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 190,045 + +0.3 190,046 - +0.4 190,059 - -0.1 190,164 + GFF469 0.19 +0.1 190,174 - GFF469 0.20 +0.0 190,176 + GFF469 0.21 -0.0 190,209 + GFF469 0.27 -1.3 190,209 + GFF469 0.27 -0.9 190,439 - GFF469 0.71 +0.4 190,492 - GFF469 0.81 -0.4 190,508 - GFF469 0.84 -1.4 190,509 + GFF469 0.84 +0.1 190,519 + GFF469 0.86 -0.5 190,520 - GFF469 0.86 -0.7 190,586 - -0.3 190,698 + -0.6 190,702 + +0.0 190,703 - +0.5 190,724 + -0.2 190,724 + -1.2 190,725 - -0.2 190,725 - +0.5 190,744 - +0.6 190,748 - -0.1 190,748 - +0.3 190,748 - -0.4 190,865 + +0.3 190,866 - +0.1 190,916 + -1.7 190,917 - -0.5 190,996 - GFF470 0.11 -2.1 191,079 - GFF470 0.16 +0.2 191,181 + GFF470 0.22 +1.9 191,181 + GFF470 0.22 -0.6 191,182 - GFF470 0.22 -0.2 191,230 + GFF470 0.25 -0.4 191,244 - GFF470 0.26 -1.1 191,244 - GFF470 0.26 -0.4 191,244 - GFF470 0.26 +0.2 191,262 + GFF470 0.27 -0.1 191,262 + GFF470 0.27 -0.3 191,263 - GFF470 0.27 +0.3 191,266 + GFF470 0.27 -0.2 191,397 - GFF470 0.35 -0.5 191,420 + GFF470 0.36 -0.7 191,457 - GFF470 0.38 -0.1 191,525 + GFF470 0.42 -0.8 191,526 - GFF470 0.42 +0.5 191,555 + GFF470 0.44 +0.1 191,555 + GFF470 0.44 +0.6 191,613 - GFF470 0.47 +0.6 191,624 - GFF470 0.48 -0.6 191,738 - GFF470 0.55 -0.7 191,752 + GFF470 0.56 +0.0 191,753 - GFF470 0.56 -1.1 191,753 - GFF470 0.56 -1.2 191,758 - GFF470 0.56 +2.0 191,840 + GFF470 0.61 +0.2 191,841 - GFF470 0.61 -0.7 191,841 - GFF470 0.61 +0.6 191,863 + GFF470 0.62 -3.7 191,864 - GFF470 0.62 +0.2 191,870 + GFF470 0.63 -0.3 192,017 + GFF470 0.71 -0.3 192,065 - GFF470 0.74 -0.2 192,110 + GFF470 0.77 -0.3 192,111 - GFF470 0.77 +0.4 192,116 - GFF470 0.77 +0.2 192,128 + GFF470 0.78 -0.1 192,128 + GFF470 0.78 +1.3 192,128 + GFF470 0.78 -0.1 192,128 + GFF470 0.78 -0.7 192,128 + GFF470 0.78 -0.6 192,142 + GFF470 0.79 -0.1 192,305 - GFF470 0.88 -0.8 192,393 + -1.0 192,393 + -0.8 192,393 + -0.2 192,393 + +2.7 192,394 - -0.2 192,394 - -0.5 192,394 - +0.5 192,396 - -1.3 192,424 + +0.8 192,424 + +0.3 192,424 + +0.2 192,425 - -0.1 192,500 + +0.1 192,577 - -0.2 192,810 - +0.2 193,129 + GFF471 0.30 +0.7 193,130 - GFF471 0.30 -0.1 193,130 - GFF471 0.30 -0.2 193,130 - GFF471 0.30 +0.1 193,274 - GFF471 0.44 -0.4
Or see this region's nucleotide sequence