Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4332 and GFF4333 are separated by 139 nucleotides GFF4333 and GFF4334 are separated by 9 nucleotides GFF4334 and GFF4335 are separated by 96 nucleotides
GFF4332 - 2-aminoethylphosphonate uptake and metabolism regulator, at 113,561 to 114,280
GFF4332
GFF4333 - 2-aminoethylphosphonate:pyruvate aminotransferase (EC 2.6.1.37), at 114,420 to 115,523
GFF4333
GFF4334 - Phosphonoacetaldehyde hydrolase (EC 3.11.1.1), at 115,533 to 116,342
GFF4334
GFF4335 - DJ-1/YajL/PfpI superfamily, includes chaperone protein YajL (former ThiJ), parkinsonism-associated protein DJ-1, peptidases PfpI, Hsp31, at 116,439 to 117,029
GFF4335
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 113.435 kb on + strand at 113.436 kb on - strand at 113.521 kb on + strand at 113.573 kb on + strand at 113.574 kb on - strand at 113.777 kb on + strand, within GFF4332 at 113.777 kb on + strand, within GFF4332 at 113.806 kb on - strand, within GFF4332 at 113.958 kb on - strand, within GFF4332 at 113.958 kb on - strand, within GFF4332 at 114.506 kb on + strand at 114.589 kb on - strand, within GFF4333 at 114.621 kb on + strand, within GFF4333 at 114.622 kb on - strand, within GFF4333 at 114.622 kb on - strand, within GFF4333 at 114.967 kb on + strand, within GFF4333 at 114.968 kb on - strand, within GFF4333 at 114.979 kb on - strand, within GFF4333 at 115.091 kb on - strand, within GFF4333 at 115.161 kb on - strand, within GFF4333 at 115.284 kb on - strand, within GFF4333 at 115.362 kb on - strand, within GFF4333 at 115.401 kb on - strand, within GFF4333 at 115.401 kb on - strand, within GFF4333 at 115.401 kb on - strand, within GFF4333 at 115.404 kb on + strand, within GFF4333 at 115.459 kb on + strand at 115.459 kb on + strand at 115.459 kb on + strand at 115.459 kb on + strand at 115.460 kb on - strand at 115.509 kb on + strand at 115.521 kb on + strand at 115.553 kb on + strand at 115.718 kb on - strand, within GFF4334 at 115.788 kb on + strand, within GFF4334 at 115.841 kb on + strand, within GFF4334 at 115.895 kb on - strand, within GFF4334 at 116.262 kb on + strand at 116.306 kb on + strand at 116.449 kb on + strand at 116.450 kb on - strand at 116.454 kb on - strand at 116.454 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 113,435 + +0.1 113,436 - +0.3 113,521 + +2.8 113,573 + +0.6 113,574 - -0.3 113,777 + GFF4332 0.30 +0.3 113,777 + GFF4332 0.30 +0.8 113,806 - GFF4332 0.34 -0.5 113,958 - GFF4332 0.55 +1.7 113,958 - GFF4332 0.55 +1.9 114,506 + -0.6 114,589 - GFF4333 0.15 -0.5 114,621 + GFF4333 0.18 +2.7 114,622 - GFF4333 0.18 +2.2 114,622 - GFF4333 0.18 +0.5 114,967 + GFF4333 0.50 -0.4 114,968 - GFF4333 0.50 +1.0 114,979 - GFF4333 0.51 +0.4 115,091 - GFF4333 0.61 +2.2 115,161 - GFF4333 0.67 +0.4 115,284 - GFF4333 0.78 -0.4 115,362 - GFF4333 0.85 -0.6 115,401 - GFF4333 0.89 +0.3 115,401 - GFF4333 0.89 -0.5 115,401 - GFF4333 0.89 -0.1 115,404 + GFF4333 0.89 -0.6 115,459 + -0.5 115,459 + -0.9 115,459 + -0.3 115,459 + -0.4 115,460 - -0.5 115,509 + +0.0 115,521 + -1.0 115,553 + +0.1 115,718 - GFF4334 0.23 +0.2 115,788 + GFF4334 0.31 +0.4 115,841 + GFF4334 0.38 -0.3 115,895 - GFF4334 0.45 +0.6 116,262 + -0.8 116,306 + +0.7 116,449 + -2.3 116,450 - -0.8 116,454 - +1.4 116,454 - +0.2
Or see this region's nucleotide sequence