Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4330 and GFF4331 are separated by 5 nucleotides GFF4331 and GFF4332 are separated by 197 nucleotides
GFF4330 - 2-aminoethylphosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 111,235 to 112,344
GFF4330
GFF4331 - 2-aminoethylphosphonate ABC transporter periplasmic binding component (TC 3.A.1.9.1), at 112,350 to 113,363
GFF4331
GFF4332 - 2-aminoethylphosphonate uptake and metabolism regulator, at 113,561 to 114,280
GFF4332
Position (kb)
112
113
114 Strain fitness (log2 ratio)
-1
0
1
2
3 at 111.632 kb on + strand, within GFF4330 at 111.632 kb on + strand, within GFF4330 at 111.633 kb on - strand, within GFF4330 at 111.679 kb on - strand, within GFF4330 at 111.731 kb on + strand, within GFF4330 at 111.786 kb on + strand, within GFF4330 at 111.787 kb on - strand, within GFF4330 at 111.787 kb on - strand, within GFF4330 at 111.789 kb on + strand, within GFF4330 at 111.789 kb on + strand, within GFF4330 at 111.969 kb on + strand, within GFF4330 at 111.969 kb on + strand, within GFF4330 at 112.082 kb on + strand, within GFF4330 at 112.262 kb on + strand at 112.385 kb on + strand at 112.466 kb on + strand, within GFF4331 at 112.468 kb on + strand, within GFF4331 at 112.468 kb on + strand, within GFF4331 at 112.469 kb on - strand, within GFF4331 at 112.794 kb on - strand, within GFF4331 at 112.835 kb on + strand, within GFF4331 at 112.835 kb on + strand, within GFF4331 at 112.982 kb on - strand, within GFF4331 at 112.982 kb on - strand, within GFF4331 at 113.235 kb on + strand, within GFF4331 at 113.367 kb on - strand at 113.369 kb on + strand at 113.419 kb on + strand at 113.435 kb on + strand at 113.436 kb on - strand at 113.521 kb on + strand at 113.573 kb on + strand at 113.574 kb on - strand at 113.777 kb on + strand, within GFF4332 at 113.777 kb on + strand, within GFF4332 at 113.806 kb on - strand, within GFF4332 at 113.958 kb on - strand, within GFF4332 at 113.958 kb on - strand, within GFF4332
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 111,632 + GFF4330 0.36 +0.1 111,632 + GFF4330 0.36 +2.0 111,633 - GFF4330 0.36 +0.1 111,679 - GFF4330 0.40 -0.1 111,731 + GFF4330 0.45 +0.1 111,786 + GFF4330 0.50 +0.6 111,787 - GFF4330 0.50 -0.7 111,787 - GFF4330 0.50 +0.6 111,789 + GFF4330 0.50 +2.1 111,789 + GFF4330 0.50 +0.5 111,969 + GFF4330 0.66 -0.4 111,969 + GFF4330 0.66 -0.7 112,082 + GFF4330 0.76 +0.4 112,262 + -0.2 112,385 + +1.3 112,466 + GFF4331 0.11 +0.0 112,468 + GFF4331 0.12 +0.7 112,468 + GFF4331 0.12 +1.1 112,469 - GFF4331 0.12 -0.2 112,794 - GFF4331 0.44 +1.0 112,835 + GFF4331 0.48 +0.3 112,835 + GFF4331 0.48 +0.0 112,982 - GFF4331 0.62 -0.3 112,982 - GFF4331 0.62 -0.3 113,235 + GFF4331 0.87 +0.1 113,367 - +2.2 113,369 + +0.5 113,419 + -0.5 113,435 + +0.1 113,436 - +0.3 113,521 + +2.8 113,573 + +0.6 113,574 - -0.3 113,777 + GFF4332 0.30 +0.3 113,777 + GFF4332 0.30 +0.8 113,806 - GFF4332 0.34 -0.5 113,958 - GFF4332 0.55 +1.7 113,958 - GFF4332 0.55 +1.9
Or see this region's nucleotide sequence