Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4166 and GFF4167 are separated by 64 nucleotides GFF4167 and GFF4168 are separated by 165 nucleotides
GFF4166 - Hemolysins and related proteins containing CBS domains, at 56,671 to 57,912
GFF4166
GFF4167 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 57,977 to 58,768
GFF4167
GFF4168 - Signal recognition particle, subunit Ffh SRP54 (TC 3.A.5.1.1), at 58,934 to 60,295
GFF4168
Position (kb) 57
58
59 Strain fitness (log2 ratio) -2
-1
0
1
2 at 56.998 kb on - strand, within GFF4166 at 57.119 kb on + strand, within GFF4166 at 57.119 kb on + strand, within GFF4166 at 57.207 kb on + strand, within GFF4166 at 57.384 kb on + strand, within GFF4166 at 57.384 kb on + strand, within GFF4166 at 57.436 kb on + strand, within GFF4166 at 57.436 kb on + strand, within GFF4166 at 57.437 kb on - strand, within GFF4166 at 57.519 kb on + strand, within GFF4166 at 57.519 kb on + strand, within GFF4166 at 57.523 kb on + strand, within GFF4166 at 57.523 kb on + strand, within GFF4166 at 57.582 kb on - strand, within GFF4166 at 57.702 kb on + strand, within GFF4166 at 57.747 kb on + strand, within GFF4166 at 57.747 kb on + strand, within GFF4166 at 57.769 kb on + strand, within GFF4166 at 57.769 kb on + strand, within GFF4166 at 57.770 kb on - strand, within GFF4166 at 57.770 kb on - strand, within GFF4166 at 57.838 kb on - strand at 57.971 kb on - strand at 57.971 kb on - strand at 57.979 kb on - strand at 57.996 kb on - strand at 57.996 kb on - strand at 58.087 kb on - strand, within GFF4167 at 58.109 kb on - strand, within GFF4167 at 58.144 kb on + strand, within GFF4167 at 58.331 kb on + strand, within GFF4167 at 58.423 kb on + strand, within GFF4167 at 58.427 kb on - strand, within GFF4167 at 58.427 kb on - strand, within GFF4167 at 58.429 kb on - strand, within GFF4167 at 58.673 kb on - strand, within GFF4167 at 58.673 kb on - strand, within GFF4167 at 58.752 kb on - strand at 58.768 kb on + strand at 58.768 kb on + strand at 58.769 kb on - strand at 58.769 kb on - strand at 58.807 kb on + strand at 58.808 kb on - strand at 58.810 kb on - strand at 58.815 kb on + strand at 58.815 kb on + strand at 58.865 kb on + strand at 58.865 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 56,998 - GFF4166 0.26 +0.7 57,119 + GFF4166 0.36 +0.3 57,119 + GFF4166 0.36 -0.3 57,207 + GFF4166 0.43 -0.2 57,384 + GFF4166 0.57 +1.7 57,384 + GFF4166 0.57 -0.0 57,436 + GFF4166 0.62 -0.3 57,436 + GFF4166 0.62 -0.3 57,437 - GFF4166 0.62 -0.0 57,519 + GFF4166 0.68 +0.5 57,519 + GFF4166 0.68 +0.0 57,523 + GFF4166 0.69 -0.2 57,523 + GFF4166 0.69 -0.2 57,582 - GFF4166 0.73 +0.1 57,702 + GFF4166 0.83 -0.4 57,747 + GFF4166 0.87 +0.3 57,747 + GFF4166 0.87 +0.3 57,769 + GFF4166 0.88 +0.2 57,769 + GFF4166 0.88 -0.1 57,770 - GFF4166 0.88 +0.8 57,770 - GFF4166 0.88 -1.5 57,838 - +0.1 57,971 - -0.2 57,971 - +2.2 57,979 - +0.6 57,996 - +0.8 57,996 - +0.1 58,087 - GFF4167 0.14 -1.1 58,109 - GFF4167 0.17 +0.8 58,144 + GFF4167 0.21 -0.9 58,331 + GFF4167 0.45 +0.1 58,423 + GFF4167 0.56 -0.4 58,427 - GFF4167 0.57 -2.0 58,427 - GFF4167 0.57 -0.4 58,429 - GFF4167 0.57 +0.6 58,673 - GFF4167 0.88 -1.1 58,673 - GFF4167 0.88 -0.9 58,752 - -0.4 58,768 + +1.0 58,768 + -0.5 58,769 - +0.1 58,769 - -0.2 58,807 + -1.7 58,808 - +1.1 58,810 - +0.5 58,815 + -0.2 58,815 + +1.0 58,865 + +0.2 58,865 + -1.3
Or see this region's nucleotide sequence