Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4081 and GFF4082 are separated by 61 nucleotides GFF4082 and GFF4083 are separated by 316 nucleotides GFF4083 and GFF4084 overlap by 8 nucleotides GFF4084 and GFF4085 overlap by 50 nucleotides GFF4085 and GFF4086 are separated by 204 nucleotides GFF4086 and GFF4087 are separated by 202 nucleotides
GFF4081 - FIG01046370: hypothetical protein, at 44,738 to 45,013
GFF4081
GFF4082 - hypothetical protein, at 45,075 to 45,194
GFF4082
GFF4083 - putative cytoplasmic protein, at 45,511 to 45,762
GFF4083
GFF4084 - FIG01045272: hypothetical protein, at 45,755 to 45,913
GFF4084
GFF4085 - FIG01046190: hypothetical protein, at 45,864 to 46,040
GFF4085
GFF4086 - hypothetical protein, at 46,245 to 46,385
GFF4086
GFF4087 - Mobile element protein, at 46,588 to 46,869
GFF4087
Position (kb) 45
46
47 Strain fitness (log2 ratio) -2
-1
0
1
2 at 45.036 kb on + strand at 45.211 kb on - strand at 45.211 kb on - strand at 45.215 kb on - strand at 45.296 kb on + strand at 46.084 kb on + strand at 46.085 kb on - strand at 46.088 kb on + strand at 46.089 kb on - strand at 46.121 kb on - strand at 46.121 kb on - strand at 46.137 kb on - strand at 46.210 kb on - strand at 46.236 kb on + strand at 46.312 kb on - strand, within GFF4086 at 46.740 kb on - strand, within GFF4087
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 45,036 + -2.0 45,211 - -0.4 45,211 - +0.3 45,215 - -0.6 45,296 + -0.2 46,084 + -0.3 46,085 - +0.8 46,088 + +0.1 46,089 - -0.6 46,121 - -0.7 46,121 - +2.0 46,137 - +0.2 46,210 - +0.0 46,236 + +0.9 46,312 - GFF4086 0.48 -0.5 46,740 - GFF4087 0.54 -0.4
Or see this region's nucleotide sequence