Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF392 and GFF393 are separated by 202 nucleotides GFF393 and GFF394 are separated by 9 nucleotides
GFF392 - ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20), at 106,830 to 107,762
GFF392
GFF393 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29), at 107,965 to 109,161
GFF393
GFF394 - L-threonine 3-dehydrogenase (EC 1.1.1.103), at 109,171 to 110,196
GFF394
Position (kb)
107
108
109
110 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 106.992 kb on - strand, within GFF392 at 107.126 kb on - strand, within GFF392 at 107.252 kb on - strand, within GFF392 at 107.404 kb on + strand, within GFF392 at 107.426 kb on - strand, within GFF392 at 107.482 kb on + strand, within GFF392 at 107.583 kb on - strand, within GFF392 at 107.781 kb on - strand at 107.822 kb on - strand at 107.827 kb on + strand at 107.869 kb on + strand at 107.930 kb on + strand at 107.931 kb on - strand at 108.048 kb on + strand at 108.096 kb on + strand, within GFF393 at 108.115 kb on - strand, within GFF393 at 108.118 kb on + strand, within GFF393 at 108.118 kb on + strand, within GFF393 at 108.118 kb on + strand, within GFF393 at 108.118 kb on + strand, within GFF393 at 108.151 kb on - strand, within GFF393 at 108.286 kb on + strand, within GFF393 at 108.349 kb on - strand, within GFF393 at 108.378 kb on + strand, within GFF393 at 108.378 kb on - strand, within GFF393 at 108.379 kb on - strand, within GFF393 at 108.422 kb on - strand, within GFF393 at 108.668 kb on + strand, within GFF393 at 108.668 kb on + strand, within GFF393 at 108.669 kb on - strand, within GFF393 at 108.672 kb on + strand, within GFF393 at 108.673 kb on - strand, within GFF393 at 108.673 kb on - strand, within GFF393 at 108.676 kb on - strand, within GFF393 at 108.776 kb on - strand, within GFF393 at 108.822 kb on + strand, within GFF393 at 109.016 kb on - strand, within GFF393 at 109.016 kb on - strand, within GFF393 at 109.016 kb on - strand, within GFF393 at 109.033 kb on + strand, within GFF393 at 109.104 kb on + strand at 109.167 kb on + strand at 109.168 kb on - strand at 109.168 kb on - strand at 109.261 kb on - strand at 109.303 kb on + strand, within GFF394 at 109.306 kb on - strand, within GFF394 at 109.363 kb on + strand, within GFF394 at 109.364 kb on - strand, within GFF394 at 109.364 kb on - strand, within GFF394 at 109.364 kb on - strand, within GFF394 at 109.364 kb on - strand, within GFF394 at 109.540 kb on + strand, within GFF394 at 109.599 kb on - strand, within GFF394 at 109.670 kb on + strand, within GFF394 at 109.974 kb on + strand, within GFF394
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 106,992 - GFF392 0.17 +0.3 107,126 - GFF392 0.32 +0.3 107,252 - GFF392 0.45 -0.1 107,404 + GFF392 0.62 -2.5 107,426 - GFF392 0.64 -0.4 107,482 + GFF392 0.70 -0.2 107,583 - GFF392 0.81 -2.0 107,781 - +0.8 107,822 - +0.9 107,827 + +0.8 107,869 + -0.5 107,930 + +0.3 107,931 - +0.3 108,048 + -0.5 108,096 + GFF393 0.11 -0.5 108,115 - GFF393 0.13 -1.0 108,118 + GFF393 0.13 -0.7 108,118 + GFF393 0.13 -0.3 108,118 + GFF393 0.13 -1.0 108,118 + GFF393 0.13 +0.2 108,151 - GFF393 0.16 -0.7 108,286 + GFF393 0.27 -0.5 108,349 - GFF393 0.32 -0.1 108,378 + GFF393 0.35 +0.4 108,378 - GFF393 0.35 -1.3 108,379 - GFF393 0.35 -0.2 108,422 - GFF393 0.38 -1.7 108,668 + GFF393 0.59 -1.6 108,668 + GFF393 0.59 -0.8 108,669 - GFF393 0.59 +0.2 108,672 + GFF393 0.59 -0.8 108,673 - GFF393 0.59 +0.4 108,673 - GFF393 0.59 -0.4 108,676 - GFF393 0.59 -0.7 108,776 - GFF393 0.68 -0.2 108,822 + GFF393 0.72 -1.6 109,016 - GFF393 0.88 +0.4 109,016 - GFF393 0.88 +0.3 109,016 - GFF393 0.88 +0.8 109,033 + GFF393 0.89 +0.3 109,104 + -0.4 109,167 + +2.2 109,168 - -0.4 109,168 - -0.6 109,261 - -0.0 109,303 + GFF394 0.13 +0.3 109,306 - GFF394 0.13 -0.7 109,363 + GFF394 0.19 +0.3 109,364 - GFF394 0.19 +0.0 109,364 - GFF394 0.19 +1.0 109,364 - GFF394 0.19 -3.4 109,364 - GFF394 0.19 -0.6 109,540 + GFF394 0.36 -0.9 109,599 - GFF394 0.42 +1.3 109,670 + GFF394 0.49 -1.6 109,974 + GFF394 0.78 +0.9
Or see this region's nucleotide sequence