Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3877

Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3875 and GFF3876 are separated by 2 nucleotidesGFF3876 and GFF3877 are separated by 2 nucleotidesGFF3877 and GFF3878 overlap by 4 nucleotides GFF3875 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 151,128 to 152,249 GFF3875 GFF3876 - GDP-L-fucose synthetase (EC 1.1.1.271), at 152,252 to 153,217 GFF3876 GFF3877 - GDP-mannose mannosyl hydrolase (EC 3.6.1.-), at 153,220 to 153,693 GFF3877 GFF3878 - Colanic acid biosysnthesis glycosyl transferase WcaI, at 153,690 to 154,913 GFF3878 Position (kb) 153 154Strain fitness (log2 ratio) -2 -1 0 1at 152.257 kb on + strandat 152.578 kb on + strand, within GFF3876at 152.579 kb on - strand, within GFF3876at 152.658 kb on - strand, within GFF3876at 152.699 kb on + strand, within GFF3876at 152.700 kb on - strand, within GFF3876at 152.914 kb on + strand, within GFF3876at 153.176 kb on + strandat 153.177 kb on - strandat 153.177 kb on - strandat 153.227 kb on + strandat 153.485 kb on - strand, within GFF3877at 153.485 kb on - strand, within GFF3877at 153.706 kb on - strandat 153.713 kb on + strandat 153.713 kb on + strandat 153.713 kb on + strandat 153.906 kb on + strand, within GFF3878at 153.906 kb on + strand, within GFF3878at 154.091 kb on - strand, within GFF3878at 154.456 kb on + strand, within GFF3878

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
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152,257 + -0.3
152,578 + GFF3876 0.34 +0.3
152,579 - GFF3876 0.34 -0.1
152,658 - GFF3876 0.42 +0.0
152,699 + GFF3876 0.46 -1.3
152,700 - GFF3876 0.46 -0.4
152,914 + GFF3876 0.69 +0.8
153,176 + -0.9
153,177 - -0.5
153,177 - -0.6
153,227 + +1.3
153,485 - GFF3877 0.56 +0.2
153,485 - GFF3877 0.56 -2.2
153,706 - -1.1
153,713 + +0.4
153,713 + +0.3
153,713 + +0.1
153,906 + GFF3878 0.18 -0.2
153,906 + GFF3878 0.18 +0.2
154,091 - GFF3878 0.33 +0.5
154,456 + GFF3878 0.63 -1.1

Or see this region's nucleotide sequence