Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3673

Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3672 and GFF3673 are separated by 43 nucleotidesGFF3673 and GFF3674 are separated by 51 nucleotidesGFF3674 and GFF3675 overlap by 4 nucleotides GFF3672 - 'Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130)' transl_table=11, at 23,780 to 24,757 GFF3672 GFF3673 - FIG004798: Putative cytoplasmic protein, at 24,801 to 26,033 GFF3673 GFF3674 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 26,085 to 26,267 GFF3674 GFF3675 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 26,264 to 27,010 GFF3675 Position (kb) 24 25 26 27Strain fitness (log2 ratio) -1 0 1 2at 25.092 kb on - strand, within GFF3673at 25.109 kb on + strand, within GFF3673at 25.141 kb on - strand, within GFF3673at 25.177 kb on + strand, within GFF3673at 25.351 kb on + strand, within GFF3673at 25.351 kb on + strand, within GFF3673at 25.352 kb on - strand, within GFF3673at 25.488 kb on + strand, within GFF3673at 25.685 kb on - strand, within GFF3673at 25.742 kb on + strand, within GFF3673at 25.792 kb on - strand, within GFF3673at 26.040 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
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25,092 - GFF3673 0.24 +1.4
25,109 + GFF3673 0.25 -1.7
25,141 - GFF3673 0.28 +0.0
25,177 + GFF3673 0.30 -0.0
25,351 + GFF3673 0.45 +0.1
25,351 + GFF3673 0.45 +2.4
25,352 - GFF3673 0.45 +1.3
25,488 + GFF3673 0.56 -1.5
25,685 - GFF3673 0.72 +0.8
25,742 + GFF3673 0.76 -1.0
25,792 - GFF3673 0.80 +0.3
26,040 - -0.2

Or see this region's nucleotide sequence