Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3136 and GFF3137 are separated by 71 nucleotides GFF3137 and GFF3138 are separated by 151 nucleotides GFF3138 and GFF3139 overlap by 4 nucleotides
GFF3136 - Uncharacterized protein ygiV, at 144,451 to 145,317
GFF3136
GFF3137 - Protein ygiW precursor, at 145,389 to 145,781
GFF3137
GFF3138 - Two-component system response regulator QseB, at 145,933 to 146,592
GFF3138
GFF3139 - Sensory histidine kinase QseC, at 146,589 to 147,938
GFF3139
Position (kb) 145
146 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 144.468 kb on - strand at 144.471 kb on + strand at 144.661 kb on - strand, within GFF3136 at 144.714 kb on + strand, within GFF3136 at 144.715 kb on - strand, within GFF3136 at 144.766 kb on - strand, within GFF3136 at 145.141 kb on - strand, within GFF3136 at 145.143 kb on - strand, within GFF3136 at 145.337 kb on - strand at 145.530 kb on - strand, within GFF3137 at 145.548 kb on - strand, within GFF3137 at 145.555 kb on + strand, within GFF3137 at 145.583 kb on + strand, within GFF3137 at 145.584 kb on - strand, within GFF3137 at 145.626 kb on - strand, within GFF3137 at 145.736 kb on + strand, within GFF3137 at 145.786 kb on - strand at 145.819 kb on - strand at 145.819 kb on - strand at 145.821 kb on + strand at 145.821 kb on + strand at 145.872 kb on + strand at 145.872 kb on + strand at 145.896 kb on - strand at 145.949 kb on + strand at 145.966 kb on - strand at 145.996 kb on - strand at 146.065 kb on + strand, within GFF3138 at 146.066 kb on - strand, within GFF3138 at 146.076 kb on + strand, within GFF3138 at 146.204 kb on + strand, within GFF3138 at 146.205 kb on - strand, within GFF3138 at 146.228 kb on + strand, within GFF3138 at 146.229 kb on - strand, within GFF3138 at 146.381 kb on + strand, within GFF3138 at 146.414 kb on + strand, within GFF3138 at 146.414 kb on + strand, within GFF3138 at 146.452 kb on - strand, within GFF3138 at 146.468 kb on - strand, within GFF3138 at 146.468 kb on - strand, within GFF3138 at 146.690 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 144,468 - -0.3 144,471 + +1.9 144,661 - GFF3136 0.24 +2.2 144,714 + GFF3136 0.30 -0.2 144,715 - GFF3136 0.30 -2.7 144,766 - GFF3136 0.36 -0.2 145,141 - GFF3136 0.80 -0.9 145,143 - GFF3136 0.80 -1.5 145,337 - -1.0 145,530 - GFF3137 0.36 -1.4 145,548 - GFF3137 0.40 -0.3 145,555 + GFF3137 0.42 -0.3 145,583 + GFF3137 0.49 -0.1 145,584 - GFF3137 0.50 +0.1 145,626 - GFF3137 0.60 -0.3 145,736 + GFF3137 0.88 -0.4 145,786 - -1.3 145,819 - -0.9 145,819 - -0.0 145,821 + +1.2 145,821 + -0.4 145,872 + -1.8 145,872 + +0.9 145,896 - -0.2 145,949 + +0.5 145,966 - -0.5 145,996 - +0.5 146,065 + GFF3138 0.20 -3.0 146,066 - GFF3138 0.20 +0.9 146,076 + GFF3138 0.22 +0.0 146,204 + GFF3138 0.41 +0.5 146,205 - GFF3138 0.41 -0.7 146,228 + GFF3138 0.45 -2.1 146,229 - GFF3138 0.45 -0.1 146,381 + GFF3138 0.68 -0.1 146,414 + GFF3138 0.73 +0.1 146,414 + GFF3138 0.73 +0.2 146,452 - GFF3138 0.79 +0.3 146,468 - GFF3138 0.81 +0.8 146,468 - GFF3138 0.81 +0.1 146,690 - -0.9
Or see this region's nucleotide sequence