Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2904 and GFF2905 are separated by 589 nucleotides GFF2905 and GFF2906 are separated by 24 nucleotides GFF2906 and GFF2907 are separated by 118 nucleotides
GFF2904 - Acetolactate synthase large subunit (EC 2.2.1.6), at 30,571 to 32,217
GFF2904
GFF2905 - MG(2+) CHELATASE FAMILY PROTEIN / ComM-related protein, at 32,807 to 34,327
GFF2905
GFF2906 - Protein yifE, at 34,352 to 34,690
GFF2906
GFF2907 - HTH-type transcriptional regulator hdfR, at 34,809 to 35,645
GFF2907
Position (kb)
32
33
34
35 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 32.038 kb on - strand, within GFF2904 at 32.048 kb on + strand, within GFF2904 at 32.114 kb on + strand at 32.114 kb on + strand at 32.115 kb on - strand at 32.137 kb on - strand at 32.161 kb on + strand at 32.194 kb on + strand at 32.218 kb on - strand at 32.646 kb on - strand at 32.646 kb on - strand at 32.646 kb on - strand at 32.806 kb on + strand at 32.807 kb on - strand at 32.866 kb on + strand at 32.866 kb on + strand at 32.867 kb on - strand at 32.867 kb on - strand at 32.867 kb on - strand at 32.868 kb on + strand at 32.868 kb on + strand at 32.869 kb on - strand at 32.869 kb on - strand at 32.878 kb on + strand at 32.932 kb on - strand at 32.953 kb on - strand at 32.953 kb on - strand at 32.957 kb on - strand at 32.957 kb on - strand at 32.969 kb on - strand, within GFF2905 at 33.094 kb on + strand, within GFF2905 at 33.095 kb on - strand, within GFF2905 at 33.116 kb on + strand, within GFF2905 at 33.142 kb on + strand, within GFF2905 at 33.251 kb on - strand, within GFF2905 at 33.252 kb on + strand, within GFF2905 at 33.252 kb on + strand, within GFF2905 at 33.272 kb on - strand, within GFF2905 at 33.286 kb on - strand, within GFF2905 at 33.572 kb on - strand, within GFF2905 at 33.631 kb on + strand, within GFF2905 at 33.631 kb on + strand, within GFF2905 at 33.632 kb on - strand, within GFF2905 at 33.632 kb on - strand, within GFF2905 at 33.638 kb on - strand, within GFF2905 at 33.757 kb on + strand, within GFF2905 at 33.758 kb on - strand, within GFF2905 at 33.759 kb on + strand, within GFF2905 at 33.760 kb on - strand, within GFF2905 at 33.781 kb on - strand, within GFF2905 at 33.825 kb on + strand, within GFF2905 at 33.900 kb on + strand, within GFF2905 at 33.900 kb on + strand, within GFF2905 at 33.900 kb on + strand, within GFF2905 at 33.900 kb on + strand, within GFF2905 at 33.900 kb on + strand, within GFF2905 at 33.901 kb on - strand, within GFF2905 at 34.059 kb on - strand, within GFF2905 at 34.113 kb on - strand, within GFF2905 at 34.197 kb on + strand at 34.228 kb on - strand at 34.273 kb on - strand at 34.351 kb on - strand at 34.372 kb on - strand at 34.448 kb on - strand, within GFF2906 at 34.449 kb on + strand, within GFF2906 at 34.449 kb on + strand, within GFF2906 at 34.450 kb on - strand, within GFF2906 at 34.502 kb on + strand, within GFF2906 at 34.642 kb on - strand, within GFF2906 at 34.704 kb on - strand at 34.739 kb on + strand at 34.884 kb on + strand at 34.884 kb on + strand at 34.914 kb on - strand, within GFF2907 at 34.970 kb on + strand, within GFF2907 at 35.028 kb on - strand, within GFF2907 at 35.114 kb on + strand, within GFF2907 at 35.114 kb on + strand, within GFF2907 at 35.143 kb on - strand, within GFF2907 at 35.150 kb on - strand, within GFF2907 at 35.290 kb on + strand, within GFF2907 at 35.311 kb on + strand, within GFF2907 at 35.314 kb on - strand, within GFF2907 at 35.314 kb on - strand, within GFF2907
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 32,038 - GFF2904 0.89 -0.8 32,048 + GFF2904 0.90 -0.1 32,114 + +0.4 32,114 + +0.3 32,115 - -0.7 32,137 - +1.9 32,161 + -0.1 32,194 + +0.7 32,218 - -0.6 32,646 - +0.5 32,646 - -0.5 32,646 - -0.2 32,806 + -0.1 32,807 - +4.2 32,866 + +0.4 32,866 + +0.3 32,867 - -0.6 32,867 - +0.2 32,867 - -0.1 32,868 + -0.2 32,868 + +0.1 32,869 - -0.5 32,869 - +0.3 32,878 + -0.2 32,932 - +0.1 32,953 - +0.5 32,953 - +0.2 32,957 - -0.4 32,957 - +0.0 32,969 - GFF2905 0.11 +0.5 33,094 + GFF2905 0.19 +1.1 33,095 - GFF2905 0.19 +1.0 33,116 + GFF2905 0.20 +0.0 33,142 + GFF2905 0.22 -0.3 33,251 - GFF2905 0.29 -1.1 33,252 + GFF2905 0.29 -1.0 33,252 + GFF2905 0.29 -0.1 33,272 - GFF2905 0.31 -0.0 33,286 - GFF2905 0.31 -0.2 33,572 - GFF2905 0.50 -0.1 33,631 + GFF2905 0.54 +1.0 33,631 + GFF2905 0.54 +0.7 33,632 - GFF2905 0.54 -0.0 33,632 - GFF2905 0.54 +1.1 33,638 - GFF2905 0.55 +1.4 33,757 + GFF2905 0.62 +1.0 33,758 - GFF2905 0.63 +0.3 33,759 + GFF2905 0.63 +0.3 33,760 - GFF2905 0.63 +2.3 33,781 - GFF2905 0.64 -0.3 33,825 + GFF2905 0.67 +1.4 33,900 + GFF2905 0.72 +0.2 33,900 + GFF2905 0.72 -0.2 33,900 + GFF2905 0.72 +0.3 33,900 + GFF2905 0.72 -0.3 33,900 + GFF2905 0.72 +0.3 33,901 - GFF2905 0.72 -0.2 34,059 - GFF2905 0.82 -2.3 34,113 - GFF2905 0.86 -0.1 34,197 + +0.1 34,228 - -0.1 34,273 - -0.2 34,351 - +0.4 34,372 - +0.0 34,448 - GFF2906 0.28 -0.3 34,449 + GFF2906 0.29 +0.3 34,449 + GFF2906 0.29 -2.4 34,450 - GFF2906 0.29 +0.1 34,502 + GFF2906 0.44 +0.5 34,642 - GFF2906 0.86 -2.7 34,704 - -0.9 34,739 + -0.6 34,884 + -2.2 34,884 + +0.6 34,914 - GFF2907 0.13 +0.1 34,970 + GFF2907 0.19 +0.3 35,028 - GFF2907 0.26 -1.5 35,114 + GFF2907 0.36 -0.1 35,114 + GFF2907 0.36 -0.3 35,143 - GFF2907 0.40 +1.0 35,150 - GFF2907 0.41 -1.3 35,290 + GFF2907 0.57 -0.7 35,311 + GFF2907 0.60 -0.4 35,314 - GFF2907 0.60 -2.1 35,314 - GFF2907 0.60 -1.5
Or see this region's nucleotide sequence