Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2803 and GFF2804 are separated by 13 nucleotides GFF2804 and GFF2805 are separated by 322 nucleotides
GFF2803 - Alanine racemase (EC 5.1.1.1) ## catabolic, at 326,874 to 327,944
GFF2803
GFF2804 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 327,958 to 329,256
GFF2804
GFF2805 - FIG004684: SpoVR-like protein, at 329,579 to 331,111
GFF2805
Position (kb) 327
328
329
330 Strain fitness (log2 ratio) -1
0
1
2 at 327.130 kb on + strand, within GFF2803 at 327.192 kb on + strand, within GFF2803 at 327.228 kb on - strand, within GFF2803 at 327.584 kb on - strand, within GFF2803 at 327.584 kb on - strand, within GFF2803 at 327.930 kb on + strand at 327.963 kb on + strand at 328.111 kb on + strand, within GFF2804 at 328.224 kb on + strand, within GFF2804 at 328.288 kb on + strand, within GFF2804 at 328.289 kb on - strand, within GFF2804 at 328.336 kb on - strand, within GFF2804 at 328.341 kb on + strand, within GFF2804 at 328.473 kb on + strand, within GFF2804 at 328.512 kb on - strand, within GFF2804 at 328.512 kb on - strand, within GFF2804 at 328.593 kb on + strand, within GFF2804 at 328.818 kb on - strand, within GFF2804 at 328.844 kb on - strand, within GFF2804 at 328.915 kb on - strand, within GFF2804 at 328.915 kb on - strand, within GFF2804 at 328.915 kb on - strand, within GFF2804 at 328.917 kb on - strand, within GFF2804 at 328.954 kb on - strand, within GFF2804 at 329.227 kb on + strand at 329.227 kb on + strand at 329.331 kb on + strand at 329.361 kb on + strand at 329.361 kb on + strand at 329.361 kb on + strand at 329.362 kb on - strand at 329.363 kb on + strand at 329.364 kb on - strand at 329.427 kb on + strand at 329.523 kb on - strand at 329.661 kb on - strand at 329.831 kb on + strand, within GFF2805 at 329.832 kb on - strand, within GFF2805 at 329.832 kb on - strand, within GFF2805 at 329.833 kb on + strand, within GFF2805 at 329.834 kb on - strand, within GFF2805 at 329.834 kb on - strand, within GFF2805 at 330.044 kb on + strand, within GFF2805 at 330.137 kb on + strand, within GFF2805 at 330.201 kb on - strand, within GFF2805
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 327,130 + GFF2803 0.24 -0.7 327,192 + GFF2803 0.30 -0.1 327,228 - GFF2803 0.33 -0.6 327,584 - GFF2803 0.66 +1.1 327,584 - GFF2803 0.66 -0.0 327,930 + -0.4 327,963 + +1.1 328,111 + GFF2804 0.12 +0.3 328,224 + GFF2804 0.20 +0.1 328,288 + GFF2804 0.25 +0.7 328,289 - GFF2804 0.25 +0.3 328,336 - GFF2804 0.29 +2.2 328,341 + GFF2804 0.29 -0.1 328,473 + GFF2804 0.40 +0.5 328,512 - GFF2804 0.43 +0.1 328,512 - GFF2804 0.43 -0.4 328,593 + GFF2804 0.49 +1.2 328,818 - GFF2804 0.66 +1.1 328,844 - GFF2804 0.68 +0.9 328,915 - GFF2804 0.74 +0.5 328,915 - GFF2804 0.74 -0.8 328,915 - GFF2804 0.74 -0.5 328,917 - GFF2804 0.74 +0.9 328,954 - GFF2804 0.77 -0.2 329,227 + -1.0 329,227 + +2.5 329,331 + +0.2 329,361 + +0.4 329,361 + -0.4 329,361 + -0.5 329,362 - -0.2 329,363 + +1.9 329,364 - -0.0 329,427 + -0.1 329,523 - +0.6 329,661 - -1.2 329,831 + GFF2805 0.16 -0.3 329,832 - GFF2805 0.17 +0.8 329,832 - GFF2805 0.17 +0.0 329,833 + GFF2805 0.17 +0.6 329,834 - GFF2805 0.17 +0.5 329,834 - GFF2805 0.17 +0.5 330,044 + GFF2805 0.30 -0.4 330,137 + GFF2805 0.36 -0.3 330,201 - GFF2805 0.41 -1.7
Or see this region's nucleotide sequence