Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2461 and GFF2462 are separated by 50 nucleotides GFF2462 and GFF2463 are separated by 90 nucleotides GFF2463 and GFF2464 are separated by 3 nucleotides
GFF2461 - Transglycosylase, Slt family, at 80,882 to 82,426
GFF2461
GFF2462 - tRNA-specific adenosine-34 deaminase (EC 3.5.4.-), at 82,477 to 82,962
GFF2462
GFF2463 - Hypothetical protein, similar to phosphoserine phosphatase, at 83,053 to 83,688
GFF2463
GFF2464 - PTS system, IIB component (EC 2.7.1.69) / PTS system, IIC component (EC 2.7.1.69), at 83,692 to 85,053
GFF2464
Position (kb)
83
84 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 82.099 kb on + strand, within GFF2461 at 82.134 kb on + strand, within GFF2461 at 82.161 kb on + strand, within GFF2461 at 82.194 kb on - strand, within GFF2461 at 82.194 kb on - strand, within GFF2461 at 82.199 kb on + strand, within GFF2461 at 82.371 kb on - strand at 82.478 kb on + strand at 83.144 kb on + strand, within GFF2463 at 83.293 kb on - strand, within GFF2463 at 83.365 kb on + strand, within GFF2463 at 83.389 kb on + strand, within GFF2463 at 83.567 kb on - strand, within GFF2463 at 83.625 kb on + strand at 83.625 kb on + strand at 83.639 kb on - strand at 83.765 kb on - strand at 83.983 kb on - strand, within GFF2464 at 83.983 kb on - strand, within GFF2464 at 83.983 kb on - strand, within GFF2464 at 84.087 kb on - strand, within GFF2464 at 84.118 kb on - strand, within GFF2464 at 84.571 kb on + strand, within GFF2464
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 82,099 + GFF2461 0.79 +1.1 82,134 + GFF2461 0.81 -0.9 82,161 + GFF2461 0.83 -0.2 82,194 - GFF2461 0.85 -1.0 82,194 - GFF2461 0.85 -0.1 82,199 + GFF2461 0.85 +0.2 82,371 - +0.6 82,478 + -0.2 83,144 + GFF2463 0.14 -2.3 83,293 - GFF2463 0.38 +0.9 83,365 + GFF2463 0.49 +1.8 83,389 + GFF2463 0.53 -0.7 83,567 - GFF2463 0.81 -0.0 83,625 + +0.6 83,625 + -0.7 83,639 - -0.3 83,765 - -0.5 83,983 - GFF2464 0.21 -1.0 83,983 - GFF2464 0.21 -0.4 83,983 - GFF2464 0.21 +0.1 84,087 - GFF2464 0.29 +0.4 84,118 - GFF2464 0.31 -0.4 84,571 + GFF2464 0.65 -0.0
Or see this region's nucleotide sequence