Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2421 and GFF2422 are separated by 208 nucleotides GFF2422 and GFF2423 are separated by 15 nucleotides GFF2423 and GFF2424 are separated by 7 nucleotides GFF2424 and GFF2425 overlap by 13 nucleotides GFF2425 and GFF2426 are separated by 54 nucleotides
GFF2421 - Alpha-2-macroglobulin, at 36,247 to 41,181
GFF2421
GFF2422 - Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1), at 41,390 to 42,232
GFF2422
GFF2423 - hypothetical protein, at 42,248 to 42,361
GFF2423
GFF2424 - hypothetical protein, at 42,369 to 42,491
GFF2424
GFF2425 - FIG074102: hypothetical protein, at 42,479 to 43,147
GFF2425
GFF2426 - hypothetical protein, at 43,202 to 43,330
GFF2426
Position (kb) 41
42
43 Strain fitness (log2 ratio) -2
-1
0
1
2 at 40.403 kb on + strand, within GFF2421 at 40.403 kb on + strand, within GFF2421 at 40.404 kb on - strand, within GFF2421 at 40.432 kb on - strand, within GFF2421 at 40.499 kb on + strand, within GFF2421 at 40.554 kb on + strand, within GFF2421 at 40.728 kb on + strand at 40.829 kb on - strand at 40.997 kb on - strand at 40.999 kb on + strand at 41.041 kb on + strand at 41.253 kb on + strand at 41.326 kb on + strand at 41.326 kb on + strand at 41.434 kb on + strand at 41.435 kb on - strand at 41.435 kb on - strand at 41.591 kb on - strand, within GFF2422 at 41.666 kb on + strand, within GFF2422 at 41.667 kb on - strand, within GFF2422 at 41.667 kb on - strand, within GFF2422 at 41.667 kb on - strand, within GFF2422 at 41.989 kb on - strand, within GFF2422 at 42.231 kb on - strand at 42.541 kb on + strand at 42.677 kb on - strand, within GFF2425 at 42.884 kb on + strand, within GFF2425
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 40,403 + GFF2421 0.84 -1.1 40,403 + GFF2421 0.84 +0.3 40,404 - GFF2421 0.84 -0.0 40,432 - GFF2421 0.85 +0.1 40,499 + GFF2421 0.86 -1.5 40,554 + GFF2421 0.87 -2.6 40,728 + -0.6 40,829 - +0.9 40,997 - -0.2 40,999 + -0.4 41,041 + +0.2 41,253 + -0.1 41,326 + +1.1 41,326 + +0.4 41,434 + -1.6 41,435 - +0.0 41,435 - -0.5 41,591 - GFF2422 0.24 -1.3 41,666 + GFF2422 0.33 -0.8 41,667 - GFF2422 0.33 -0.9 41,667 - GFF2422 0.33 -0.2 41,667 - GFF2422 0.33 -0.5 41,989 - GFF2422 0.71 +0.3 42,231 - +1.8 42,541 + +0.8 42,677 - GFF2425 0.30 -0.2 42,884 + GFF2425 0.61 -0.2
Or see this region's nucleotide sequence