Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF230 and GFF231 are separated by 107 nucleotides GFF231 and GFF232 are separated by 368 nucleotides GFF232 and GFF233 are separated by 444 nucleotides GFF233 and GFF234 are separated by 69 nucleotides
GFF230 - Adenylate cyclase (EC 4.6.1.1), at 5,201 to 7,747
GFF230
GFF231 - Putative inner membrane protein, at 7,855 to 8,208
GFF231
GFF232 - Putative inner membrane protein, at 8,577 to 8,936
GFF232
GFF233 - Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly, at 9,381 to 9,701
GFF233
GFF234 - STMD1.44 protein, at 9,771 to 10,160
GFF234
Position (kb) 8
9 Strain fitness (log2 ratio) -1
0
1 at 7.587 kb on - strand at 7.610 kb on - strand at 7.796 kb on - strand at 7.918 kb on - strand, within GFF231 at 7.928 kb on + strand, within GFF231 at 7.928 kb on + strand, within GFF231 at 7.928 kb on + strand, within GFF231 at 8.098 kb on + strand, within GFF231 at 8.540 kb on + strand at 8.653 kb on + strand, within GFF232 at 8.653 kb on + strand, within GFF232 at 8.660 kb on - strand, within GFF232 at 8.726 kb on + strand, within GFF232 at 9.020 kb on + strand at 9.070 kb on + strand at 9.171 kb on + strand at 9.549 kb on - strand, within GFF233 at 9.551 kb on + strand, within GFF233 at 9.552 kb on - strand, within GFF233 at 9.795 kb on - strand at 9.812 kb on - strand, within GFF234 at 9.839 kb on + strand, within GFF234 at 9.843 kb on + strand, within GFF234 at 9.843 kb on + strand, within GFF234
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 7,587 - -0.9 7,610 - +1.0 7,796 - -0.0 7,918 - GFF231 0.18 -0.2 7,928 + GFF231 0.21 -0.3 7,928 + GFF231 0.21 +0.1 7,928 + GFF231 0.21 -0.2 8,098 + GFF231 0.69 -0.7 8,540 + +0.5 8,653 + GFF232 0.21 +1.6 8,653 + GFF232 0.21 -0.1 8,660 - GFF232 0.23 +0.4 8,726 + GFF232 0.41 -0.3 9,020 + -0.2 9,070 + +0.0 9,171 + +0.1 9,549 - GFF233 0.52 -0.1 9,551 + GFF233 0.53 -0.3 9,552 - GFF233 0.53 -0.5 9,795 - +1.4 9,812 - GFF234 0.11 -0.0 9,839 + GFF234 0.17 +0.5 9,843 + GFF234 0.18 -0.3 9,843 + GFF234 0.18 +0.6
Or see this region's nucleotide sequence