Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2299 and GFF2300 are separated by 43 nucleotides GFF2300 and GFF2301 are separated by 9 nucleotides GFF2301 and GFF2302 are separated by 91 nucleotides GFF2302 and GFF2303 are separated by 112 nucleotides
GFF2299 - Autoinducer 2 (AI-2) aldolase LsrF (EC 4.2.1.-), at 87,075 to 87,950
GFF2299
GFF2300 - Autoinducer 2 (AI-2) modifying protein LsrG, at 87,994 to 88,323
GFF2300
GFF2301 - Ribulose-phosphate 3-epimerase (EC 5.1.3.1), at 88,333 to 89,097
GFF2301
GFF2302 - Triosephosphate isomerase (EC 5.3.1.1), at 89,189 to 89,956
GFF2302
GFF2303 - Putative uncharacterized protein YiiQ, at 90,069 to 90,665
GFF2303
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-1
0
1 at 87.349 kb on + strand, within GFF2299 at 87.510 kb on - strand, within GFF2299 at 87.539 kb on + strand, within GFF2299 at 87.542 kb on + strand, within GFF2299 at 87.611 kb on + strand, within GFF2299 at 87.687 kb on + strand, within GFF2299 at 87.688 kb on - strand, within GFF2299 at 87.713 kb on + strand, within GFF2299 at 87.823 kb on + strand, within GFF2299 at 87.827 kb on + strand, within GFF2299 at 87.827 kb on + strand, within GFF2299 at 87.836 kb on + strand, within GFF2299 at 87.836 kb on + strand, within GFF2299 at 87.837 kb on - strand, within GFF2299 at 87.880 kb on + strand at 88.399 kb on - strand at 88.442 kb on - strand, within GFF2301 at 88.518 kb on + strand, within GFF2301 at 88.679 kb on + strand, within GFF2301 at 88.679 kb on + strand, within GFF2301 at 88.679 kb on + strand, within GFF2301 at 88.679 kb on + strand, within GFF2301 at 88.680 kb on - strand, within GFF2301 at 88.744 kb on - strand, within GFF2301 at 88.748 kb on + strand, within GFF2301 at 88.820 kb on + strand, within GFF2301 at 88.820 kb on + strand, within GFF2301 at 88.820 kb on + strand, within GFF2301 at 88.995 kb on + strand, within GFF2301 at 89.004 kb on - strand, within GFF2301 at 89.055 kb on + strand at 89.056 kb on - strand at 89.082 kb on - strand at 89.095 kb on + strand at 89.096 kb on - strand at 89.096 kb on - strand at 89.121 kb on - strand at 89.130 kb on - strand at 89.133 kb on + strand at 89.133 kb on + strand at 89.190 kb on + strand at 89.683 kb on + strand, within GFF2302 at 89.986 kb on - strand at 90.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 87,349 + GFF2299 0.31 -0.7 87,510 - GFF2299 0.50 +0.4 87,539 + GFF2299 0.53 -0.0 87,542 + GFF2299 0.53 +0.0 87,611 + GFF2299 0.61 -0.2 87,687 + GFF2299 0.70 -0.2 87,688 - GFF2299 0.70 +1.7 87,713 + GFF2299 0.73 +0.3 87,823 + GFF2299 0.85 -0.2 87,827 + GFF2299 0.86 -0.4 87,827 + GFF2299 0.86 -0.0 87,836 + GFF2299 0.87 -0.7 87,836 + GFF2299 0.87 +0.4 87,837 - GFF2299 0.87 -0.2 87,880 + +0.0 88,399 - -1.0 88,442 - GFF2301 0.14 -0.1 88,518 + GFF2301 0.24 +0.4 88,679 + GFF2301 0.45 -0.0 88,679 + GFF2301 0.45 +0.3 88,679 + GFF2301 0.45 -0.0 88,679 + GFF2301 0.45 +0.2 88,680 - GFF2301 0.45 -0.4 88,744 - GFF2301 0.54 +0.1 88,748 + GFF2301 0.54 +0.2 88,820 + GFF2301 0.64 -0.2 88,820 + GFF2301 0.64 +1.7 88,820 + GFF2301 0.64 -0.1 88,995 + GFF2301 0.87 +0.2 89,004 - GFF2301 0.88 +0.3 89,055 + -0.1 89,056 - -0.1 89,082 - -0.3 89,095 + -0.4 89,096 - +0.6 89,096 - +0.8 89,121 - -0.3 89,130 - +1.4 89,133 + -0.2 89,133 + +0.0 89,190 + +0.3 89,683 + GFF2302 0.64 -0.8 89,986 - +0.5 90,033 - -0.2
Or see this region's nucleotide sequence