Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2279 and GFF2280 overlap by 4 nucleotides GFF2280 and GFF2281 are separated by 150 nucleotides GFF2281 and GFF2282 are separated by 147 nucleotides
GFF2279 - Copper sensory histidine kinase CpxA, at 67,300 to 68,673
GFF2279
GFF2280 - Copper-sensing two-component system response regulator CpxR, at 68,670 to 69,368
GFF2280
GFF2281 - P pilus assembly/Cpx signaling pathway, periplasmic inhibitor/zinc-resistance associated protein, at 69,519 to 70,019
GFF2281
GFF2282 - Cobalt-zinc-cadmium resistance protein, at 70,167 to 71,069
GFF2282
Position (kb) 69
70
71 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 68.612 kb on - strand at 68.612 kb on - strand at 68.692 kb on + strand at 68.769 kb on - strand, within GFF2280 at 68.873 kb on - strand, within GFF2280 at 69.063 kb on - strand, within GFF2280 at 69.144 kb on - strand, within GFF2280 at 69.186 kb on - strand, within GFF2280 at 69.373 kb on + strand at 69.395 kb on + strand at 69.414 kb on - strand at 69.426 kb on + strand at 69.427 kb on - strand at 69.437 kb on - strand at 69.593 kb on + strand, within GFF2281 at 69.593 kb on + strand, within GFF2281 at 69.635 kb on + strand, within GFF2281 at 69.651 kb on - strand, within GFF2281 at 69.651 kb on - strand, within GFF2281 at 69.705 kb on - strand, within GFF2281 at 69.705 kb on - strand, within GFF2281 at 69.705 kb on - strand, within GFF2281 at 69.705 kb on - strand, within GFF2281 at 69.879 kb on + strand, within GFF2281 at 70.115 kb on + strand at 70.115 kb on + strand at 70.137 kb on + strand at 70.137 kb on + strand at 70.138 kb on - strand at 70.166 kb on + strand at 70.263 kb on + strand, within GFF2282 at 70.281 kb on - strand, within GFF2282 at 70.428 kb on - strand, within GFF2282 at 70.469 kb on + strand, within GFF2282 at 70.551 kb on - strand, within GFF2282 at 70.553 kb on + strand, within GFF2282 at 70.554 kb on - strand, within GFF2282 at 70.554 kb on - strand, within GFF2282 at 70.582 kb on + strand, within GFF2282 at 70.617 kb on - strand, within GFF2282 at 70.740 kb on + strand, within GFF2282 at 70.742 kb on + strand, within GFF2282 at 70.751 kb on - strand, within GFF2282 at 70.751 kb on - strand, within GFF2282 at 70.762 kb on - strand, within GFF2282 at 70.780 kb on + strand, within GFF2282 at 71.009 kb on + strand at 71.009 kb on + strand at 71.010 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 68,612 - +1.9 68,612 - -1.2 68,692 + -0.4 68,769 - GFF2280 0.14 +1.4 68,873 - GFF2280 0.29 +2.2 69,063 - GFF2280 0.56 -1.6 69,144 - GFF2280 0.68 +1.2 69,186 - GFF2280 0.74 +0.9 69,373 + +2.1 69,395 + +0.9 69,414 - -0.4 69,426 + -0.4 69,427 - -0.6 69,437 - -0.4 69,593 + GFF2281 0.15 -0.1 69,593 + GFF2281 0.15 -0.4 69,635 + GFF2281 0.23 -0.0 69,651 - GFF2281 0.26 +0.0 69,651 - GFF2281 0.26 +0.9 69,705 - GFF2281 0.37 +0.1 69,705 - GFF2281 0.37 -0.4 69,705 - GFF2281 0.37 -0.1 69,705 - GFF2281 0.37 -0.8 69,879 + GFF2281 0.72 -0.7 70,115 + +0.7 70,115 + -0.5 70,137 + -0.7 70,137 + -0.3 70,138 - -0.3 70,166 + +0.4 70,263 + GFF2282 0.11 -0.4 70,281 - GFF2282 0.13 +0.2 70,428 - GFF2282 0.29 +0.4 70,469 + GFF2282 0.33 -0.3 70,551 - GFF2282 0.43 -0.3 70,553 + GFF2282 0.43 -0.1 70,554 - GFF2282 0.43 -0.8 70,554 - GFF2282 0.43 +0.5 70,582 + GFF2282 0.46 -0.5 70,617 - GFF2282 0.50 -0.5 70,740 + GFF2282 0.63 -0.7 70,742 + GFF2282 0.64 +0.2 70,751 - GFF2282 0.65 -0.1 70,751 - GFF2282 0.65 -0.0 70,762 - GFF2282 0.66 +0.5 70,780 + GFF2282 0.68 -0.4 71,009 + +0.2 71,009 + +1.8 71,010 - -3.3
Or see this region's nucleotide sequence