Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF204 and GFF205 are separated by 67 nucleotides GFF205 and GFF206 are separated by 227 nucleotides GFF206 and GFF207 are separated by 34 nucleotides
GFF204 - Electron transfer flavoprotein, beta subunit FixA, at 199,636 to 200,406
GFF204
GFF205 - FIG01045916: hypothetical protein, at 200,474 to 200,659
GFF205
GFF206 - L-carnitine/gamma-butyrobetaine antiporter, at 200,887 to 202,404
GFF206
GFF207 - Crotonobetainyl-CoA dehydrogenase (EC 1.3.99.-), at 202,439 to 203,581
GFF207
Position (kb) 200
201
202
203 Strain fitness (log2 ratio) -2
-1
0
1
2 at 199.931 kb on - strand, within GFF204 at 200.058 kb on + strand, within GFF204 at 200.059 kb on - strand, within GFF204 at 200.063 kb on - strand, within GFF204 at 200.770 kb on + strand at 201.022 kb on + strand at 201.022 kb on + strand at 201.022 kb on + strand at 201.058 kb on + strand, within GFF206 at 201.058 kb on + strand, within GFF206 at 201.060 kb on + strand, within GFF206 at 201.224 kb on - strand, within GFF206 at 201.224 kb on - strand, within GFF206 at 201.226 kb on + strand, within GFF206 at 201.227 kb on - strand, within GFF206 at 201.229 kb on + strand, within GFF206 at 201.230 kb on - strand, within GFF206 at 201.230 kb on - strand, within GFF206 at 201.231 kb on + strand, within GFF206 at 201.232 kb on - strand, within GFF206 at 201.334 kb on + strand, within GFF206 at 201.334 kb on + strand, within GFF206 at 201.334 kb on + strand, within GFF206 at 201.335 kb on - strand, within GFF206 at 201.337 kb on - strand, within GFF206 at 201.472 kb on + strand, within GFF206 at 201.473 kb on - strand, within GFF206 at 201.540 kb on + strand, within GFF206 at 201.541 kb on - strand, within GFF206 at 201.568 kb on - strand, within GFF206 at 201.673 kb on - strand, within GFF206 at 201.676 kb on + strand, within GFF206 at 201.676 kb on + strand, within GFF206 at 201.739 kb on + strand, within GFF206 at 201.739 kb on + strand, within GFF206 at 201.740 kb on - strand, within GFF206 at 201.740 kb on - strand, within GFF206 at 201.794 kb on - strand, within GFF206 at 201.918 kb on - strand, within GFF206 at 202.168 kb on + strand, within GFF206 at 202.227 kb on - strand, within GFF206 at 202.351 kb on - strand at 202.403 kb on - strand at 202.409 kb on + strand at 202.520 kb on + strand at 202.520 kb on + strand at 202.520 kb on + strand at 202.551 kb on - strand at 202.701 kb on - strand, within GFF207 at 202.807 kb on + strand, within GFF207 at 202.808 kb on - strand, within GFF207 at 202.841 kb on - strand, within GFF207 at 202.841 kb on - strand, within GFF207 at 202.857 kb on - strand, within GFF207 at 202.894 kb on + strand, within GFF207 at 202.904 kb on - strand, within GFF207 at 202.919 kb on + strand, within GFF207 at 202.919 kb on + strand, within GFF207 at 202.922 kb on - strand, within GFF207 at 202.928 kb on + strand, within GFF207 at 202.929 kb on - strand, within GFF207 at 203.183 kb on + strand, within GFF207 at 203.183 kb on + strand, within GFF207 at 203.184 kb on - strand, within GFF207 at 203.393 kb on + strand, within GFF207
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 199,931 - GFF204 0.38 -0.8 200,058 + GFF204 0.55 -0.5 200,059 - GFF204 0.55 -0.4 200,063 - GFF204 0.55 -0.8 200,770 + -0.1 201,022 + +0.1 201,022 + -0.8 201,022 + -0.7 201,058 + GFF206 0.11 +2.6 201,058 + GFF206 0.11 -0.1 201,060 + GFF206 0.11 -1.5 201,224 - GFF206 0.22 -1.9 201,224 - GFF206 0.22 +0.4 201,226 + GFF206 0.22 -1.3 201,227 - GFF206 0.22 -0.2 201,229 + GFF206 0.23 -0.2 201,230 - GFF206 0.23 -0.3 201,230 - GFF206 0.23 -0.2 201,231 + GFF206 0.23 -1.2 201,232 - GFF206 0.23 -0.3 201,334 + GFF206 0.29 -0.4 201,334 + GFF206 0.29 +0.4 201,334 + GFF206 0.29 +0.3 201,335 - GFF206 0.30 +0.9 201,337 - GFF206 0.30 +0.4 201,472 + GFF206 0.39 +0.2 201,473 - GFF206 0.39 +1.4 201,540 + GFF206 0.43 +1.9 201,541 - GFF206 0.43 +0.4 201,568 - GFF206 0.45 +1.9 201,673 - GFF206 0.52 +0.8 201,676 + GFF206 0.52 +0.3 201,676 + GFF206 0.52 +1.1 201,739 + GFF206 0.56 +0.5 201,739 + GFF206 0.56 -1.9 201,740 - GFF206 0.56 -0.3 201,740 - GFF206 0.56 -1.0 201,794 - GFF206 0.60 -0.1 201,918 - GFF206 0.68 +2.3 202,168 + GFF206 0.84 +1.9 202,227 - GFF206 0.88 +0.2 202,351 - +0.1 202,403 - -0.5 202,409 + +0.7 202,520 + +0.4 202,520 + +1.3 202,520 + +1.4 202,551 - -0.1 202,701 - GFF207 0.23 -1.7 202,807 + GFF207 0.32 -0.3 202,808 - GFF207 0.32 +0.2 202,841 - GFF207 0.35 +0.5 202,841 - GFF207 0.35 -0.5 202,857 - GFF207 0.37 -0.7 202,894 + GFF207 0.40 -1.0 202,904 - GFF207 0.41 -0.6 202,919 + GFF207 0.42 -0.3 202,919 + GFF207 0.42 +1.3 202,922 - GFF207 0.42 -0.7 202,928 + GFF207 0.43 -0.1 202,929 - GFF207 0.43 -0.2 203,183 + GFF207 0.65 -1.5 203,183 + GFF207 0.65 +0.4 203,184 - GFF207 0.65 +0.3 203,393 + GFF207 0.83 -0.3
Or see this region's nucleotide sequence