Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF200 and GFF201 are separated by 80 nucleotides GFF201 and GFF202 overlap by 4 nucleotides GFF202 and GFF203 are separated by 49 nucleotides
GFF200 - Putative metabolite transport protein yaaU, at 195,572 to 196,978
GFF200
GFF201 - Ferredoxin-like protein FixX, at 197,059 to 197,346
GFF201
GFF202 - Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-), at 197,343 to 198,629
GFF202
GFF203 - Electron transfer flavoprotein, alpha subunit FixB, at 198,679 to 199,620
GFF203
Position (kb) 197
198
199 Strain fitness (log2 ratio) -2
-1
0
1
2 at 196.543 kb on - strand, within GFF200 at 196.556 kb on - strand, within GFF200 at 196.883 kb on - strand at 197.057 kb on + strand at 197.204 kb on + strand, within GFF201 at 197.349 kb on + strand at 197.708 kb on + strand, within GFF202 at 197.740 kb on - strand, within GFF202 at 197.769 kb on - strand, within GFF202 at 197.769 kb on - strand, within GFF202 at 197.769 kb on - strand, within GFF202 at 197.804 kb on - strand, within GFF202 at 198.024 kb on + strand, within GFF202 at 198.312 kb on + strand, within GFF202 at 198.313 kb on - strand, within GFF202 at 198.470 kb on + strand, within GFF202 at 198.471 kb on - strand, within GFF202 at 198.478 kb on + strand, within GFF202 at 198.617 kb on + strand at 198.629 kb on + strand at 198.629 kb on + strand at 198.629 kb on + strand at 198.629 kb on + strand at 198.630 kb on - strand at 198.630 kb on - strand at 198.630 kb on - strand at 198.630 kb on - strand at 198.634 kb on - strand at 198.634 kb on - strand at 198.634 kb on - strand at 198.634 kb on - strand at 198.653 kb on + strand at 198.654 kb on - strand at 198.854 kb on + strand, within GFF203 at 198.855 kb on - strand, within GFF203 at 199.245 kb on + strand, within GFF203 at 199.421 kb on + strand, within GFF203 at 199.540 kb on + strand at 199.541 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 196,543 - GFF200 0.69 +0.3 196,556 - GFF200 0.70 -1.0 196,883 - +0.9 197,057 + -0.2 197,204 + GFF201 0.50 -0.8 197,349 + +0.1 197,708 + GFF202 0.28 +0.4 197,740 - GFF202 0.31 -0.3 197,769 - GFF202 0.33 +0.2 197,769 - GFF202 0.33 -0.2 197,769 - GFF202 0.33 +0.2 197,804 - GFF202 0.36 -0.6 198,024 + GFF202 0.53 +0.6 198,312 + GFF202 0.75 +0.4 198,313 - GFF202 0.75 +0.1 198,470 + GFF202 0.88 +1.1 198,471 - GFF202 0.88 +0.9 198,478 + GFF202 0.88 +0.6 198,617 + +0.2 198,629 + +0.1 198,629 + +2.6 198,629 + +0.7 198,629 + +0.7 198,630 - +0.1 198,630 - -0.6 198,630 - -0.3 198,630 - -1.2 198,634 - -0.3 198,634 - -0.2 198,634 - +0.0 198,634 - -0.3 198,653 + +0.5 198,654 - -0.2 198,854 + GFF203 0.19 +1.0 198,855 - GFF203 0.19 +0.4 199,245 + GFF203 0.60 +0.5 199,421 + GFF203 0.79 +1.9 199,540 + -2.4 199,541 - -0.6
Or see this region's nucleotide sequence