Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1562 and GFF1563 are separated by 163 nucleotides GFF1563 and GFF1564 are separated by 25 nucleotides GFF1564 and GFF1565 are separated by 25 nucleotides
GFF1562 - Tricarboxylate transport membrane protein TctA, at 24,206 to 25,720
GFF1562
GFF1563 - hypothetical protein, at 25,884 to 25,997
GFF1563
GFF1564 - Carbon starvation induced protein CsiD, at 26,023 to 27,000
GFF1564
GFF1565 - L-2-hydroxyglutarate oxidase (EC 1.1.3.15), at 27,026 to 28,294
GFF1565
Position (kb) 26
27
28 Strain fitness (log2 ratio) -1
0
1
2
3 at 25.042 kb on + strand, within GFF1562 at 25.046 kb on + strand, within GFF1562 at 25.046 kb on + strand, within GFF1562 at 25.047 kb on - strand, within GFF1562 at 25.048 kb on + strand, within GFF1562 at 25.049 kb on - strand, within GFF1562 at 25.421 kb on + strand, within GFF1562 at 25.422 kb on - strand, within GFF1562 at 25.422 kb on - strand, within GFF1562 at 25.492 kb on + strand, within GFF1562 at 25.591 kb on + strand at 25.741 kb on + strand at 25.741 kb on + strand at 25.741 kb on + strand at 25.742 kb on - strand at 25.755 kb on - strand at 25.805 kb on - strand at 26.077 kb on - strand at 26.210 kb on + strand, within GFF1564 at 26.210 kb on + strand, within GFF1564 at 26.210 kb on + strand, within GFF1564 at 26.210 kb on + strand, within GFF1564 at 26.258 kb on + strand, within GFF1564 at 26.402 kb on - strand, within GFF1564 at 26.422 kb on + strand, within GFF1564 at 26.467 kb on + strand, within GFF1564 at 26.512 kb on - strand, within GFF1564 at 26.515 kb on + strand, within GFF1564 at 26.516 kb on - strand, within GFF1564 at 26.537 kb on - strand, within GFF1564 at 26.537 kb on - strand, within GFF1564 at 26.569 kb on - strand, within GFF1564 at 26.752 kb on - strand, within GFF1564 at 26.752 kb on - strand, within GFF1564 at 26.998 kb on + strand at 26.998 kb on + strand at 26.998 kb on + strand at 27.086 kb on - strand at 27.415 kb on + strand, within GFF1565 at 27.524 kb on + strand, within GFF1565 at 27.525 kb on - strand, within GFF1565 at 27.546 kb on + strand, within GFF1565 at 27.736 kb on + strand, within GFF1565 at 27.750 kb on - strand, within GFF1565
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 25,042 + GFF1562 0.55 +0.6 25,046 + GFF1562 0.55 -0.2 25,046 + GFF1562 0.55 -0.2 25,047 - GFF1562 0.56 -0.1 25,048 + GFF1562 0.56 -0.1 25,049 - GFF1562 0.56 +0.0 25,421 + GFF1562 0.80 -0.2 25,422 - GFF1562 0.80 +0.4 25,422 - GFF1562 0.80 -0.2 25,492 + GFF1562 0.85 +0.5 25,591 + +0.3 25,741 + -0.5 25,741 + +0.0 25,741 + +0.5 25,742 - +1.5 25,755 - +0.4 25,805 - -0.1 26,077 - +1.9 26,210 + GFF1564 0.19 +0.3 26,210 + GFF1564 0.19 +0.9 26,210 + GFF1564 0.19 +0.3 26,210 + GFF1564 0.19 +1.0 26,258 + GFF1564 0.24 -0.1 26,402 - GFF1564 0.39 +1.8 26,422 + GFF1564 0.41 -0.1 26,467 + GFF1564 0.45 -0.2 26,512 - GFF1564 0.50 -1.1 26,515 + GFF1564 0.50 +0.3 26,516 - GFF1564 0.50 +0.3 26,537 - GFF1564 0.53 -0.2 26,537 - GFF1564 0.53 +0.4 26,569 - GFF1564 0.56 -0.6 26,752 - GFF1564 0.75 +2.1 26,752 - GFF1564 0.75 -0.6 26,998 + -0.4 26,998 + +1.0 26,998 + -0.1 27,086 - +2.8 27,415 + GFF1565 0.31 +0.1 27,524 + GFF1565 0.39 +0.6 27,525 - GFF1565 0.39 +1.5 27,546 + GFF1565 0.41 +0.4 27,736 + GFF1565 0.56 +0.3 27,750 - GFF1565 0.57 -0.2
Or see this region's nucleotide sequence