Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1322 and GFF1323 are separated by 9 nucleotides GFF1323 and GFF1324 are separated by 117 nucleotides
GFF1322 - Galactose operon repressor, GalR-LacI family of transcriptional regulators, at 30,474 to 31,493
GFF1322
GFF1323 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 31,503 to 32,765
GFF1323
GFF1324 - Transcriptional activator protein LysR, at 32,883 to 33,818
GFF1324
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 30.770 kb on + strand, within GFF1322 at 31.023 kb on - strand, within GFF1322 at 31.023 kb on - strand, within GFF1322 at 31.037 kb on + strand, within GFF1322 at 31.060 kb on + strand, within GFF1322 at 31.132 kb on - strand, within GFF1322 at 31.198 kb on + strand, within GFF1322 at 31.199 kb on - strand, within GFF1322 at 31.208 kb on + strand, within GFF1322 at 31.258 kb on + strand, within GFF1322 at 31.259 kb on - strand, within GFF1322 at 31.345 kb on + strand, within GFF1322 at 31.610 kb on - strand at 31.610 kb on - strand at 31.633 kb on + strand, within GFF1323 at 31.654 kb on - strand, within GFF1323 at 31.654 kb on - strand, within GFF1323 at 31.817 kb on + strand, within GFF1323 at 31.826 kb on - strand, within GFF1323 at 31.826 kb on - strand, within GFF1323 at 31.830 kb on - strand, within GFF1323 at 31.834 kb on + strand, within GFF1323 at 32.046 kb on - strand, within GFF1323 at 32.198 kb on + strand, within GFF1323 at 32.198 kb on + strand, within GFF1323 at 32.225 kb on - strand, within GFF1323 at 32.390 kb on + strand, within GFF1323 at 32.417 kb on + strand, within GFF1323 at 32.418 kb on - strand, within GFF1323 at 32.586 kb on - strand, within GFF1323 at 32.586 kb on - strand, within GFF1323 at 32.750 kb on - strand at 32.765 kb on + strand at 32.766 kb on - strand at 32.883 kb on - strand at 33.055 kb on + strand, within GFF1324 at 33.094 kb on + strand, within GFF1324 at 33.220 kb on + strand, within GFF1324 at 33.261 kb on - strand, within GFF1324 at 33.370 kb on + strand, within GFF1324 at 33.371 kb on - strand, within GFF1324 at 33.394 kb on + strand, within GFF1324 at 33.506 kb on + strand, within GFF1324 at 33.511 kb on + strand, within GFF1324 at 33.612 kb on - strand, within GFF1324 at 33.744 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 30,770 + GFF1322 0.29 +0.2 31,023 - GFF1322 0.54 +0.2 31,023 - GFF1322 0.54 +0.7 31,037 + GFF1322 0.55 +0.4 31,060 + GFF1322 0.57 +0.2 31,132 - GFF1322 0.65 -0.1 31,198 + GFF1322 0.71 -1.1 31,199 - GFF1322 0.71 +0.7 31,208 + GFF1322 0.72 -0.1 31,258 + GFF1322 0.77 -0.5 31,259 - GFF1322 0.77 -0.3 31,345 + GFF1322 0.85 -0.9 31,610 - -0.3 31,610 - +4.4 31,633 + GFF1323 0.10 +0.5 31,654 - GFF1323 0.12 -0.7 31,654 - GFF1323 0.12 -0.1 31,817 + GFF1323 0.25 -0.4 31,826 - GFF1323 0.26 -0.1 31,826 - GFF1323 0.26 -0.0 31,830 - GFF1323 0.26 +0.1 31,834 + GFF1323 0.26 +0.2 32,046 - GFF1323 0.43 -0.1 32,198 + GFF1323 0.55 +1.2 32,198 + GFF1323 0.55 -1.5 32,225 - GFF1323 0.57 -1.2 32,390 + GFF1323 0.70 +0.2 32,417 + GFF1323 0.72 +0.4 32,418 - GFF1323 0.72 -0.8 32,586 - GFF1323 0.86 +0.2 32,586 - GFF1323 0.86 -1.1 32,750 - -0.4 32,765 + -0.4 32,766 - -0.1 32,883 - -0.0 33,055 + GFF1324 0.18 -0.2 33,094 + GFF1324 0.23 +1.2 33,220 + GFF1324 0.36 +0.1 33,261 - GFF1324 0.40 +0.5 33,370 + GFF1324 0.52 -0.7 33,371 - GFF1324 0.52 +0.3 33,394 + GFF1324 0.55 -1.1 33,506 + GFF1324 0.67 -0.3 33,511 + GFF1324 0.67 +0.1 33,612 - GFF1324 0.78 -1.7 33,744 - -3.4
Or see this region's nucleotide sequence