Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF117 and GFF118 are separated by 37 nucleotides GFF118 and GFF119 are separated by 82 nucleotides GFF119 and GFF120 are separated by 86 nucleotides GFF120 and GFF121 are separated by 199 nucleotides
GFF117 - FIG01045811: hypothetical protein, at 105,257 to 106,048
GFF117
GFF118 - Probable secreted protein, at 106,086 to 106,370
GFF118
GFF119 - hypothetical protein, at 106,453 to 106,572
GFF119
GFF120 - Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4) @ Dihydrolipoamide dehydrogenase (EC 1.8.1.4), at 106,659 to 108,083
GFF120
GFF121 - Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12), at 108,283 to 110,172
GFF121
Position (kb)
106
107
108
109 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 105.672 kb on - strand, within GFF117 at 105.672 kb on - strand, within GFF117 at 105.693 kb on - strand, within GFF117 at 105.699 kb on + strand, within GFF117 at 105.759 kb on - strand, within GFF117 at 105.857 kb on - strand, within GFF117 at 105.955 kb on + strand, within GFF117 at 105.956 kb on - strand, within GFF117 at 105.960 kb on - strand, within GFF117 at 106.003 kb on + strand at 106.003 kb on + strand at 106.437 kb on + strand at 106.495 kb on + strand, within GFF119 at 106.660 kb on + strand at 106.701 kb on - strand at 108.284 kb on + strand at 108.285 kb on - strand at 108.315 kb on + strand at 108.315 kb on + strand at 108.448 kb on - strand at 108.448 kb on - strand at 108.529 kb on - strand, within GFF121 at 108.529 kb on - strand, within GFF121 at 108.807 kb on - strand, within GFF121
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 105,672 - GFF117 0.52 -0.4 105,672 - GFF117 0.52 -0.1 105,693 - GFF117 0.55 +0.1 105,699 + GFF117 0.56 +0.2 105,759 - GFF117 0.63 -0.5 105,857 - GFF117 0.76 +1.0 105,955 + GFF117 0.88 +0.4 105,956 - GFF117 0.88 -0.6 105,960 - GFF117 0.89 -0.3 106,003 + -2.3 106,003 + +0.4 106,437 + +0.1 106,495 + GFF119 0.35 -0.9 106,660 + +1.3 106,701 - +1.0 108,284 + +2.1 108,285 - +1.4 108,315 + +2.2 108,315 + +1.3 108,448 - +4.7 108,448 - +0.9 108,529 - GFF121 0.13 +2.7 108,529 - GFF121 0.13 +3.3 108,807 - GFF121 0.28 +2.7
Or see this region's nucleotide sequence