Experiment: LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1178 and GFF1179 are separated by 113 nucleotides GFF1179 and GFF1180 overlap by 14 nucleotides GFF1180 and GFF1181 overlap by 11 nucleotides GFF1181 and GFF1182 overlap by 4 nucleotides
GFF1178 - hypothetical protein, at 23,689 to 23,820
GFF1178
GFF1179 - IncF plasmid conjugative transfer protein TraQ, at 23,934 to 24,227
GFF1179
GFF1180 - IncF plasmid conjugative transfer protein TrbB, at 24,214 to 24,762
GFF1180
GFF1181 - IncF plasmid conjugative transfer pilus assembly protein TraH, at 24,752 to 26,134
GFF1181
GFF1182 - IncF plasmid conjugative transfer protein TraG, at 26,131 to 28,953
GFF1182
Position (kb)
24
25
26
27 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 23.905 kb on - strand at 23.907 kb on - strand at 24.226 kb on - strand at 24.303 kb on + strand, within GFF1180 at 24.489 kb on - strand, within GFF1180 at 24.512 kb on - strand, within GFF1180 at 24.534 kb on + strand, within GFF1180 at 24.671 kb on + strand, within GFF1180 at 24.672 kb on - strand, within GFF1180 at 24.739 kb on + strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.740 kb on - strand at 24.760 kb on + strand at 24.772 kb on + strand at 24.772 kb on + strand at 24.786 kb on + strand at 24.787 kb on - strand at 24.789 kb on + strand at 24.862 kb on + strand at 24.924 kb on - strand, within GFF1181 at 24.942 kb on + strand, within GFF1181 at 24.943 kb on - strand, within GFF1181 at 24.943 kb on - strand, within GFF1181 at 24.943 kb on - strand, within GFF1181 at 24.943 kb on - strand, within GFF1181 at 24.943 kb on - strand, within GFF1181 at 24.945 kb on - strand, within GFF1181 at 25.029 kb on - strand, within GFF1181 at 25.029 kb on - strand, within GFF1181 at 25.087 kb on + strand, within GFF1181 at 25.088 kb on - strand, within GFF1181 at 25.093 kb on + strand, within GFF1181 at 25.189 kb on + strand, within GFF1181 at 25.195 kb on + strand, within GFF1181 at 25.195 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.339 kb on + strand, within GFF1181 at 25.340 kb on - strand, within GFF1181 at 25.565 kb on - strand, within GFF1181 at 25.600 kb on - strand, within GFF1181 at 25.600 kb on - strand, within GFF1181 at 25.601 kb on + strand, within GFF1181 at 25.601 kb on + strand, within GFF1181 at 25.602 kb on - strand, within GFF1181 at 25.602 kb on - strand, within GFF1181 at 25.621 kb on + strand, within GFF1181 at 25.747 kb on + strand, within GFF1181 at 25.762 kb on + strand, within GFF1181 at 25.763 kb on - strand, within GFF1181 at 25.839 kb on - strand, within GFF1181 at 25.853 kb on + strand, within GFF1181 at 25.853 kb on + strand, within GFF1181 at 25.854 kb on - strand, within GFF1181 at 25.880 kb on + strand, within GFF1181 at 25.881 kb on - strand, within GFF1181 at 25.881 kb on - strand, within GFF1181 at 25.931 kb on + strand, within GFF1181 at 25.931 kb on + strand, within GFF1181 at 25.932 kb on - strand, within GFF1181 at 25.932 kb on - strand, within GFF1181 at 25.954 kb on + strand, within GFF1181 at 25.954 kb on + strand, within GFF1181 at 25.955 kb on - strand, within GFF1181 at 26.058 kb on - strand at 26.058 kb on - strand at 26.143 kb on + strand at 26.144 kb on - strand at 26.144 kb on - strand at 26.173 kb on - strand at 26.173 kb on - strand at 26.268 kb on + strand at 26.269 kb on - strand at 26.286 kb on + strand at 26.287 kb on - strand at 26.287 kb on - strand at 26.331 kb on - strand at 26.360 kb on + strand at 26.360 kb on + strand at 26.361 kb on - strand at 26.367 kb on - strand at 26.367 kb on - strand at 26.406 kb on + strand at 26.476 kb on + strand, within GFF1182 at 26.477 kb on - strand, within GFF1182 at 26.477 kb on - strand, within GFF1182 at 26.477 kb on - strand, within GFF1182 at 26.512 kb on + strand, within GFF1182 at 26.513 kb on - strand, within GFF1182 at 26.526 kb on + strand, within GFF1182 at 26.527 kb on - strand, within GFF1182 at 26.609 kb on - strand, within GFF1182 at 26.634 kb on + strand, within GFF1182 at 26.635 kb on - strand, within GFF1182 at 26.635 kb on - strand, within GFF1182 at 26.635 kb on - strand, within GFF1182 at 26.654 kb on - strand, within GFF1182 at 26.692 kb on - strand, within GFF1182 at 26.731 kb on + strand, within GFF1182 at 26.731 kb on + strand, within GFF1182 at 26.803 kb on - strand, within GFF1182 at 26.830 kb on + strand, within GFF1182 at 26.831 kb on - strand, within GFF1182 at 26.909 kb on - strand, within GFF1182 at 26.950 kb on - strand, within GFF1182 at 26.950 kb on - strand, within GFF1182 at 26.991 kb on + strand, within GFF1182 at 27.120 kb on + strand, within GFF1182 at 27.120 kb on + strand, within GFF1182
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (LP_new) 15 MOI remove 23,905 - -0.1 23,907 - -0.3 24,226 - +0.0 24,303 + GFF1180 0.16 -0.4 24,489 - GFF1180 0.50 +1.8 24,512 - GFF1180 0.54 +0.0 24,534 + GFF1180 0.58 +2.2 24,671 + GFF1180 0.83 +0.5 24,672 - GFF1180 0.83 -0.2 24,739 + -0.7 24,740 - -0.5 24,740 - +1.5 24,740 - -0.1 24,740 - +0.0 24,740 - -0.9 24,760 + +0.1 24,772 + +2.0 24,772 + -2.2 24,786 + +0.1 24,787 - +1.4 24,789 + +0.0 24,862 + +1.1 24,924 - GFF1181 0.12 -1.4 24,942 + GFF1181 0.14 -0.4 24,943 - GFF1181 0.14 +0.1 24,943 - GFF1181 0.14 -1.3 24,943 - GFF1181 0.14 -0.3 24,943 - GFF1181 0.14 +0.0 24,943 - GFF1181 0.14 -0.4 24,945 - GFF1181 0.14 -0.5 25,029 - GFF1181 0.20 -0.2 25,029 - GFF1181 0.20 -0.5 25,087 + GFF1181 0.24 -0.3 25,088 - GFF1181 0.24 +2.0 25,093 + GFF1181 0.25 -0.7 25,189 + GFF1181 0.32 -0.0 25,195 + GFF1181 0.32 -0.5 25,195 + GFF1181 0.32 -0.7 25,339 + GFF1181 0.42 -0.6 25,339 + GFF1181 0.42 +0.1 25,339 + GFF1181 0.42 -0.1 25,339 + GFF1181 0.42 -0.2 25,340 - GFF1181 0.43 +0.0 25,565 - GFF1181 0.59 +1.6 25,600 - GFF1181 0.61 +0.2 25,600 - GFF1181 0.61 +0.6 25,601 + GFF1181 0.61 +0.8 25,601 + GFF1181 0.61 -0.7 25,602 - GFF1181 0.61 -0.3 25,602 - GFF1181 0.61 -0.8 25,621 + GFF1181 0.63 -2.6 25,747 + GFF1181 0.72 -0.1 25,762 + GFF1181 0.73 -0.9 25,763 - GFF1181 0.73 +0.6 25,839 - GFF1181 0.79 -0.9 25,853 + GFF1181 0.80 +0.4 25,853 + GFF1181 0.80 +0.4 25,854 - GFF1181 0.80 -0.2 25,880 + GFF1181 0.82 -0.7 25,881 - GFF1181 0.82 -0.4 25,881 - GFF1181 0.82 -2.4 25,931 + GFF1181 0.85 -0.5 25,931 + GFF1181 0.85 -1.2 25,932 - GFF1181 0.85 +1.8 25,932 - GFF1181 0.85 -1.4 25,954 + GFF1181 0.87 -0.1 25,954 + GFF1181 0.87 -0.4 25,955 - GFF1181 0.87 +2.6 26,058 - +0.9 26,058 - -0.4 26,143 + +0.2 26,144 - -1.5 26,144 - -1.0 26,173 - -0.8 26,173 - -0.2 26,268 + -0.2 26,269 - -1.0 26,286 + +0.7 26,287 - -0.5 26,287 - -0.5 26,331 - +0.5 26,360 + -0.1 26,360 + -0.3 26,361 - -0.1 26,367 - +1.3 26,367 - -0.4 26,406 + +0.1 26,476 + GFF1182 0.12 +0.2 26,477 - GFF1182 0.12 +1.3 26,477 - GFF1182 0.12 -0.2 26,477 - GFF1182 0.12 -1.1 26,512 + GFF1182 0.13 -0.0 26,513 - GFF1182 0.14 -1.9 26,526 + GFF1182 0.14 -0.0 26,527 - GFF1182 0.14 -0.8 26,609 - GFF1182 0.17 -1.5 26,634 + GFF1182 0.18 +0.0 26,635 - GFF1182 0.18 -0.7 26,635 - GFF1182 0.18 +0.2 26,635 - GFF1182 0.18 -0.1 26,654 - GFF1182 0.19 -0.4 26,692 - GFF1182 0.20 -0.2 26,731 + GFF1182 0.21 +1.0 26,731 + GFF1182 0.21 +0.1 26,803 - GFF1182 0.24 -0.1 26,830 + GFF1182 0.25 -0.2 26,831 - GFF1182 0.25 -0.4 26,909 - GFF1182 0.28 -0.0 26,950 - GFF1182 0.29 +0.1 26,950 - GFF1182 0.29 -0.3 26,991 + GFF1182 0.30 +1.5 27,120 + GFF1182 0.35 -0.0 27,120 + GFF1182 0.35 -0.4
Or see this region's nucleotide sequence