Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5159 and Atu5160 are separated by 360 nucleotides Atu5160 and Atu5161 are separated by 93 nucleotides Atu5161 and Atu5162 are separated by 2 nucleotides Atu5162 and Atu5163 overlap by 4 nucleotides Atu5163 and Atu5164 are separated by 2 nucleotides
Atu5159: Atu5159 - hypothetical protein, at 158,745 to 159,149
Atu5159
Atu5160: Atu5160 - transcriptional regulator protein, at 159,510 to 159,872
Atu5160
Atu5161: Atu5161 - hypothetical protein, at 159,966 to 160,505
Atu5161
Atu5162: Atu5162 - type IV secretion protein AvhB1, at 160,508 to 161,194
Atu5162
Atu5163: Atu5163 - type IV secretion protein AvhB2, at 161,191 to 161,490
Atu5163
Atu5164: Atu5164 - type IV secretion protein AvhB3, at 161,493 to 161,834
Atu5164
Position (kb) 159
160
161 Strain fitness (log2 ratio) -1
0
1
2 at 159.016 kb on - strand, within Atu5159 at 159.102 kb on - strand, within Atu5159 at 159.148 kb on + strand at 159.151 kb on - strand at 159.162 kb on + strand at 159.178 kb on - strand at 159.179 kb on - strand at 159.262 kb on + strand at 159.314 kb on + strand at 159.317 kb on - strand at 159.322 kb on - strand at 159.354 kb on + strand at 159.359 kb on - strand at 159.361 kb on + strand at 159.361 kb on + strand at 159.362 kb on - strand at 159.391 kb on - strand at 159.455 kb on - strand at 159.486 kb on - strand at 159.486 kb on - strand at 159.496 kb on - strand at 159.543 kb on - strand at 159.655 kb on - strand, within Atu5160 at 159.766 kb on + strand, within Atu5160 at 159.833 kb on + strand, within Atu5160 at 159.833 kb on + strand, within Atu5160 at 159.842 kb on - strand at 159.953 kb on - strand at 160.006 kb on + strand at 160.066 kb on + strand, within Atu5161 at 160.074 kb on - strand, within Atu5161 at 160.079 kb on - strand, within Atu5161 at 160.096 kb on + strand, within Atu5161 at 160.136 kb on + strand, within Atu5161 at 160.181 kb on + strand, within Atu5161 at 160.206 kb on - strand, within Atu5161 at 160.342 kb on + strand, within Atu5161 at 160.387 kb on + strand, within Atu5161 at 160.395 kb on - strand, within Atu5161 at 160.420 kb on - strand, within Atu5161 at 160.420 kb on - strand, within Atu5161 at 160.431 kb on + strand, within Atu5161 at 160.449 kb on + strand, within Atu5161 at 160.492 kb on + strand at 160.492 kb on + strand at 160.507 kb on - strand at 160.511 kb on + strand at 160.547 kb on - strand at 160.621 kb on + strand, within Atu5162 at 160.656 kb on + strand, within Atu5162 at 160.656 kb on + strand, within Atu5162 at 160.664 kb on - strand, within Atu5162 at 160.664 kb on - strand, within Atu5162 at 160.747 kb on - strand, within Atu5162 at 160.749 kb on + strand, within Atu5162 at 160.790 kb on + strand, within Atu5162 at 160.849 kb on + strand, within Atu5162 at 160.968 kb on - strand, within Atu5162 at 161.128 kb on - strand at 161.129 kb on + strand at 161.208 kb on + strand at 161.399 kb on + strand, within Atu5163 at 161.404 kb on + strand, within Atu5163 at 161.404 kb on + strand, within Atu5163 at 161.412 kb on - strand, within Atu5163 at 161.484 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 159,016 - Atu5159 0.67 +0.8 159,102 - Atu5159 0.88 +2.5 159,148 + -0.0 159,151 - +1.0 159,162 + +1.0 159,178 - +0.4 159,179 - +0.7 159,262 + -0.7 159,314 + +0.9 159,317 - +0.8 159,322 - -0.1 159,354 + +0.3 159,359 - +0.7 159,361 + +0.7 159,361 + +0.0 159,362 - +0.3 159,391 - -0.6 159,455 - -1.3 159,486 - +0.5 159,486 - -0.9 159,496 - +0.3 159,543 - +2.7 159,655 - Atu5160 0.40 +1.1 159,766 + Atu5160 0.71 +0.8 159,833 + Atu5160 0.89 +1.1 159,833 + Atu5160 0.89 +2.4 159,842 - +1.5 159,953 - +0.5 160,006 + +0.1 160,066 + Atu5161 0.19 -0.1 160,074 - Atu5161 0.20 +1.1 160,079 - Atu5161 0.21 -0.2 160,096 + Atu5161 0.24 +1.8 160,136 + Atu5161 0.31 +0.4 160,181 + Atu5161 0.40 +0.9 160,206 - Atu5161 0.44 -0.2 160,342 + Atu5161 0.70 -0.1 160,387 + Atu5161 0.78 +0.9 160,395 - Atu5161 0.79 -0.0 160,420 - Atu5161 0.84 +0.0 160,420 - Atu5161 0.84 +1.8 160,431 + Atu5161 0.86 +1.9 160,449 + Atu5161 0.89 -0.1 160,492 + -0.0 160,492 + +0.7 160,507 - +1.0 160,511 + +1.6 160,547 - -0.2 160,621 + Atu5162 0.16 +1.3 160,656 + Atu5162 0.22 +0.3 160,656 + Atu5162 0.22 -0.5 160,664 - Atu5162 0.23 +0.9 160,664 - Atu5162 0.23 -0.2 160,747 - Atu5162 0.35 +0.0 160,749 + Atu5162 0.35 -0.3 160,790 + Atu5162 0.41 +0.1 160,849 + Atu5162 0.50 -0.2 160,968 - Atu5162 0.67 -0.4 161,128 - +1.8 161,129 + +0.5 161,208 + +0.8 161,399 + Atu5163 0.69 +0.8 161,404 + Atu5163 0.71 +1.6 161,404 + Atu5163 0.71 -0.2 161,412 - Atu5163 0.74 +0.8 161,484 + +1.4
Or see this region's nucleotide sequence