Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF961 and GFF962 overlap by 4 nucleotides GFF962 and GFF963 overlap by 14 nucleotides
GFF961 - putative cytoplasmic protein, at 142,124 to 144,727
GFF961
GFF962 - putative cytoplasmic protein, at 144,724 to 145,536
GFF962
GFF963 - putative ABC-type sugar/spermidine/putrescine transport system ATPase component, at 145,523 to 146,614
GFF963
Position (kb)
144
145
146 Strain fitness (log2 ratio)
-1
0
1
2 at 143.737 kb on + strand, within GFF961 at 143.925 kb on + strand, within GFF961 at 143.954 kb on - strand, within GFF961 at 144.257 kb on + strand, within GFF961 at 144.867 kb on + strand, within GFF962 at 144.919 kb on - strand, within GFF962 at 144.934 kb on + strand, within GFF962 at 144.935 kb on - strand, within GFF962 at 145.041 kb on + strand, within GFF962 at 145.042 kb on - strand, within GFF962 at 145.150 kb on + strand, within GFF962 at 145.252 kb on + strand, within GFF962 at 145.503 kb on + strand at 145.587 kb on + strand at 145.722 kb on - strand, within GFF963 at 145.722 kb on - strand, within GFF963 at 145.740 kb on - strand, within GFF963 at 145.802 kb on + strand, within GFF963 at 145.802 kb on + strand, within GFF963 at 145.978 kb on + strand, within GFF963 at 145.992 kb on + strand, within GFF963 at 145.993 kb on - strand, within GFF963 at 145.993 kb on - strand, within GFF963 at 145.993 kb on - strand, within GFF963 at 146.096 kb on - strand, within GFF963 at 146.123 kb on + strand, within GFF963 at 146.124 kb on - strand, within GFF963 at 146.124 kb on - strand, within GFF963 at 146.144 kb on - strand, within GFF963 at 146.168 kb on - strand, within GFF963 at 146.169 kb on + strand, within GFF963 at 146.170 kb on - strand, within GFF963 at 146.170 kb on - strand, within GFF963 at 146.198 kb on - strand, within GFF963 at 146.198 kb on - strand, within GFF963 at 146.313 kb on - strand, within GFF963 at 146.395 kb on - strand, within GFF963 at 146.410 kb on + strand, within GFF963
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 143,737 + GFF961 0.62 -0.2 143,925 + GFF961 0.69 +2.6 143,954 - GFF961 0.70 -1.2 144,257 + GFF961 0.82 -1.4 144,867 + GFF962 0.18 -0.1 144,919 - GFF962 0.24 +0.2 144,934 + GFF962 0.26 +1.3 144,935 - GFF962 0.26 +0.1 145,041 + GFF962 0.39 +0.6 145,042 - GFF962 0.39 +0.9 145,150 + GFF962 0.52 +0.0 145,252 + GFF962 0.65 -0.2 145,503 + -0.6 145,587 + +0.9 145,722 - GFF963 0.18 +1.2 145,722 - GFF963 0.18 -0.7 145,740 - GFF963 0.20 +1.8 145,802 + GFF963 0.26 +0.3 145,802 + GFF963 0.26 +0.0 145,978 + GFF963 0.42 -0.4 145,992 + GFF963 0.43 +0.1 145,993 - GFF963 0.43 +0.8 145,993 - GFF963 0.43 -0.7 145,993 - GFF963 0.43 +0.8 146,096 - GFF963 0.52 +1.0 146,123 + GFF963 0.55 +0.6 146,124 - GFF963 0.55 -1.0 146,124 - GFF963 0.55 +0.1 146,144 - GFF963 0.57 +0.2 146,168 - GFF963 0.59 -0.7 146,169 + GFF963 0.59 +0.2 146,170 - GFF963 0.59 +1.6 146,170 - GFF963 0.59 -0.3 146,198 - GFF963 0.62 +0.6 146,198 - GFF963 0.62 +1.2 146,313 - GFF963 0.72 -0.2 146,395 - GFF963 0.80 +0.0 146,410 + GFF963 0.81 -0.4
Or see this region's nucleotide sequence