Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF942 and GFF943 are separated by 32 nucleotides GFF943 and GFF944 are separated by 78 nucleotides GFF944 and GFF945 are separated by 106 nucleotides GFF945 and GFF946 are separated by 194 nucleotides
GFF942 - tRNA-(ms[2]io[6]A)-hydroxylase (EC 1.-.-.-), at 116,745 to 117,509
GFF942
GFF943 - FIG01046694: hypothetical protein, at 117,542 to 117,664
GFF943
GFF944 - putative exported protein, at 117,743 to 118,231
GFF944
GFF945 - Aspartate N-acetyltransferase (EC 2.3.1.17), at 118,338 to 118,841
GFF945
GFF946 - Inner membrane protein YjgN, at 119,036 to 120,223
GFF946
Position (kb) 118
119 Strain fitness (log2 ratio) -2
-1
0
1 at 117.364 kb on - strand, within GFF942 at 117.372 kb on + strand, within GFF942 at 117.373 kb on - strand, within GFF942 at 117.373 kb on - strand, within GFF942 at 117.439 kb on + strand at 117.452 kb on + strand at 117.452 kb on + strand at 117.452 kb on + strand at 117.453 kb on - strand at 117.672 kb on + strand at 117.740 kb on + strand at 118.679 kb on - strand, within GFF945 at 118.718 kb on + strand, within GFF945 at 119.508 kb on - strand, within GFF946 at 119.519 kb on + strand, within GFF946 at 119.620 kb on + strand, within GFF946 at 119.692 kb on + strand, within GFF946 at 119.770 kb on - strand, within GFF946
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 117,364 - GFF942 0.81 -0.4 117,372 + GFF942 0.82 -0.1 117,373 - GFF942 0.82 +0.1 117,373 - GFF942 0.82 -0.4 117,439 + +0.2 117,452 + +0.1 117,452 + +0.5 117,452 + -0.1 117,453 - -1.9 117,672 + -0.5 117,740 + +1.1 118,679 - GFF945 0.68 -1.2 118,718 + GFF945 0.75 -0.5 119,508 - GFF946 0.40 -1.6 119,519 + GFF946 0.41 +0.8 119,620 + GFF946 0.49 +0.1 119,692 + GFF946 0.55 +0.9 119,770 - GFF946 0.62 +1.6
Or see this region's nucleotide sequence