Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF895 and GFF896 are separated by 194 nucleotides GFF896 and GFF897 are separated by 29 nucleotides GFF897 and GFF898 overlap by 29 nucleotides GFF898 and GFF899 are separated by 228 nucleotides
GFF895 - 5-keto-2-deoxygluconokinase (EC 2.7.1.92) / uncharacterized domain, at 75,766 to 77,697
GFF895
GFF896 - hypothetical protein, at 77,892 to 78,005
GFF896
GFF897 - hypothetical protein, at 78,035 to 78,169
GFF897
GFF898 - Epi-inositol hydrolase (EC 3.7.1.-), at 78,141 to 80,060
GFF898
GFF899 - Myo-inositol 2-dehydrogenase 1 (EC 1.1.1.18), at 80,289 to 81,263
GFF899
Position (kb) 78
79
80
81 Strain fitness (log2 ratio) -1
0
1
2 at 77.210 kb on + strand, within GFF895 at 77.211 kb on - strand, within GFF895 at 77.211 kb on - strand, within GFF895 at 77.351 kb on + strand, within GFF895 at 77.521 kb on + strand at 77.574 kb on - strand at 77.593 kb on - strand at 77.593 kb on - strand at 77.625 kb on - strand at 77.703 kb on + strand at 78.035 kb on - strand at 78.073 kb on + strand, within GFF897 at 78.073 kb on + strand, within GFF897 at 78.074 kb on - strand, within GFF897 at 78.074 kb on - strand, within GFF897 at 78.118 kb on - strand, within GFF897 at 78.118 kb on - strand, within GFF897 at 78.177 kb on + strand at 78.410 kb on + strand, within GFF898 at 78.478 kb on - strand, within GFF898 at 78.605 kb on - strand, within GFF898 at 78.618 kb on - strand, within GFF898 at 79.089 kb on + strand, within GFF898 at 79.090 kb on - strand, within GFF898 at 79.094 kb on - strand, within GFF898 at 79.203 kb on + strand, within GFF898 at 79.203 kb on + strand, within GFF898 at 79.204 kb on - strand, within GFF898 at 79.248 kb on + strand, within GFF898 at 79.248 kb on + strand, within GFF898 at 79.249 kb on - strand, within GFF898 at 79.250 kb on + strand, within GFF898 at 79.322 kb on + strand, within GFF898 at 79.322 kb on + strand, within GFF898 at 79.329 kb on - strand, within GFF898 at 79.526 kb on + strand, within GFF898 at 79.587 kb on + strand, within GFF898 at 79.700 kb on + strand, within GFF898 at 79.701 kb on - strand, within GFF898 at 79.706 kb on + strand, within GFF898 at 79.707 kb on - strand, within GFF898 at 79.738 kb on - strand, within GFF898 at 79.828 kb on - strand, within GFF898 at 79.915 kb on + strand at 79.916 kb on - strand at 79.922 kb on - strand at 79.949 kb on + strand at 79.949 kb on + strand at 79.950 kb on - strand at 79.950 kb on - strand at 79.954 kb on - strand at 80.119 kb on - strand at 80.450 kb on + strand, within GFF899 at 80.951 kb on - strand, within GFF899 at 80.952 kb on + strand, within GFF899 at 80.952 kb on + strand, within GFF899 at 80.953 kb on - strand, within GFF899 at 80.953 kb on - strand, within GFF899 at 80.953 kb on - strand, within GFF899 at 81.015 kb on - strand, within GFF899
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 77,210 + GFF895 0.75 +0.4 77,211 - GFF895 0.75 -0.0 77,211 - GFF895 0.75 +0.1 77,351 + GFF895 0.82 +0.3 77,521 + -0.7 77,574 - +0.8 77,593 - -0.4 77,593 - +0.4 77,625 - -0.7 77,703 + -0.6 78,035 - +0.1 78,073 + GFF897 0.28 -1.5 78,073 + GFF897 0.28 -0.3 78,074 - GFF897 0.29 +0.4 78,074 - GFF897 0.29 -0.0 78,118 - GFF897 0.61 +1.1 78,118 - GFF897 0.61 -0.5 78,177 + +0.2 78,410 + GFF898 0.14 -0.1 78,478 - GFF898 0.18 -0.3 78,605 - GFF898 0.24 -0.4 78,618 - GFF898 0.25 -0.5 79,089 + GFF898 0.49 -0.2 79,090 - GFF898 0.49 -0.6 79,094 - GFF898 0.50 -0.3 79,203 + GFF898 0.55 -0.9 79,203 + GFF898 0.55 +0.1 79,204 - GFF898 0.55 -0.4 79,248 + GFF898 0.58 +0.4 79,248 + GFF898 0.58 -0.5 79,249 - GFF898 0.58 +1.2 79,250 + GFF898 0.58 -0.2 79,322 + GFF898 0.62 +0.0 79,322 + GFF898 0.62 -0.8 79,329 - GFF898 0.62 -0.5 79,526 + GFF898 0.72 +0.0 79,587 + GFF898 0.75 -0.2 79,700 + GFF898 0.81 -0.2 79,701 - GFF898 0.81 +0.3 79,706 + GFF898 0.82 +1.6 79,707 - GFF898 0.82 +0.2 79,738 - GFF898 0.83 +0.2 79,828 - GFF898 0.88 +0.2 79,915 + -0.4 79,916 - -0.7 79,922 - -1.5 79,949 + -0.1 79,949 + -0.6 79,950 - -0.0 79,950 - -0.5 79,954 - +2.2 80,119 - -0.5 80,450 + GFF899 0.17 +0.4 80,951 - GFF899 0.68 -0.9 80,952 + GFF899 0.68 +1.0 80,952 + GFF899 0.68 +0.2 80,953 - GFF899 0.68 +0.2 80,953 - GFF899 0.68 -0.5 80,953 - GFF899 0.68 -0.1 81,015 - GFF899 0.74 -0.3
Or see this region's nucleotide sequence