Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF727 and GFF728 are separated by 32 nucleotides GFF728 and GFF729 are separated by 60 nucleotides GFF729 and GFF730 are separated by 2 nucleotides
GFF727 - Dipeptide transport ATP-binding protein DppD in protein degradation cluster, at 85,165 to 87,036
GFF727
GFF728 - Dipeptide transport system, periplasmic component in protein degradation cluster, at 87,069 to 88,607
GFF728
GFF729 - Dipeptide transport system permease protein DppB in protein degradation cluster, at 88,668 to 89,588
GFF729
GFF730 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 89,591 to 90,502
GFF730
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 86.100 kb on + strand, within GFF727 at 86.100 kb on + strand, within GFF727 at 86.100 kb on + strand, within GFF727 at 86.379 kb on + strand, within GFF727 at 86.721 kb on + strand, within GFF727 at 86.733 kb on + strand, within GFF727 at 86.734 kb on - strand, within GFF727 at 86.777 kb on - strand, within GFF727 at 86.777 kb on - strand, within GFF727 at 86.839 kb on + strand, within GFF727 at 86.910 kb on - strand at 86.910 kb on - strand at 86.921 kb on + strand at 87.032 kb on + strand at 87.032 kb on + strand at 87.033 kb on - strand at 87.035 kb on - strand at 87.090 kb on - strand at 87.198 kb on + strand at 87.213 kb on - strand at 87.303 kb on + strand, within GFF728 at 87.719 kb on + strand, within GFF728 at 87.867 kb on - strand, within GFF728 at 87.876 kb on + strand, within GFF728 at 87.876 kb on + strand, within GFF728 at 87.877 kb on - strand, within GFF728 at 87.877 kb on - strand, within GFF728 at 87.878 kb on + strand, within GFF728 at 87.885 kb on - strand, within GFF728 at 87.911 kb on + strand, within GFF728 at 87.940 kb on - strand, within GFF728 at 88.208 kb on - strand, within GFF728 at 88.284 kb on + strand, within GFF728 at 88.285 kb on - strand, within GFF728 at 88.285 kb on - strand, within GFF728 at 88.285 kb on - strand, within GFF728 at 88.459 kb on - strand at 88.479 kb on - strand at 88.605 kb on + strand at 88.605 kb on + strand at 88.606 kb on - strand at 88.606 kb on - strand at 88.609 kb on + strand at 88.610 kb on - strand at 88.677 kb on + strand at 88.678 kb on - strand at 88.702 kb on + strand at 88.709 kb on + strand at 88.983 kb on - strand, within GFF729 at 88.983 kb on - strand, within GFF729 at 88.987 kb on + strand, within GFF729 at 89.278 kb on - strand, within GFF729 at 89.326 kb on + strand, within GFF729 at 89.426 kb on + strand, within GFF729 at 89.582 kb on + strand at 89.583 kb on - strand at 89.586 kb on + strand at 89.586 kb on + strand at 89.586 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 86,100 + GFF727 0.50 -0.5 86,100 + GFF727 0.50 -0.2 86,100 + GFF727 0.50 +0.5 86,379 + GFF727 0.65 +0.5 86,721 + GFF727 0.83 +0.3 86,733 + GFF727 0.84 -0.5 86,734 - GFF727 0.84 -0.1 86,777 - GFF727 0.86 +2.9 86,777 - GFF727 0.86 +0.5 86,839 + GFF727 0.89 -1.0 86,910 - -1.6 86,910 - -0.1 86,921 + +0.0 87,032 + -0.3 87,032 + +0.5 87,033 - -2.1 87,035 - -0.0 87,090 - -0.6 87,198 + -0.5 87,213 - -0.5 87,303 + GFF728 0.15 -0.7 87,719 + GFF728 0.42 -0.6 87,867 - GFF728 0.52 -0.1 87,876 + GFF728 0.52 -1.4 87,876 + GFF728 0.52 -3.8 87,877 - GFF728 0.53 -1.2 87,877 - GFF728 0.53 -1.2 87,878 + GFF728 0.53 -0.6 87,885 - GFF728 0.53 +0.0 87,911 + GFF728 0.55 -0.2 87,940 - GFF728 0.57 -2.6 88,208 - GFF728 0.74 +0.9 88,284 + GFF728 0.79 -0.7 88,285 - GFF728 0.79 -0.5 88,285 - GFF728 0.79 -0.2 88,285 - GFF728 0.79 +0.1 88,459 - -0.1 88,479 - -1.7 88,605 + -0.8 88,605 + -0.8 88,606 - +0.3 88,606 - -0.5 88,609 + -0.9 88,610 - -0.8 88,677 + -0.9 88,678 - +0.1 88,702 + -2.2 88,709 + -0.5 88,983 - GFF729 0.34 -1.2 88,983 - GFF729 0.34 +0.6 88,987 + GFF729 0.35 +1.8 89,278 - GFF729 0.66 +2.1 89,326 + GFF729 0.71 -0.7 89,426 + GFF729 0.82 -0.6 89,582 + +0.6 89,583 - +0.1 89,586 + -0.2 89,586 + +0.0 89,586 + -0.5
Or see this region's nucleotide sequence