Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF69 and GFF70 overlap by 1 nucleotides GFF70 and GFF71 overlap by 1 nucleotides GFF71 and GFF72 overlap by 4 nucleotides GFF72 and GFF73 are separated by 18 nucleotides
GFF69 - Uncharacterized protein YadU in stf fimbrial cluster, at 51,986 to 52,825
GFF69
GFF70 - Minor fimbrial subunit StfG, at 52,825 to 53,355
GFF70
GFF71 - Minor fimbrial subunit StfF, at 53,355 to 53,831
GFF71
GFF72 - Minor fimbrial subunit StfE, at 53,828 to 54,340
GFF72
GFF73 - Periplasmic fimbrial chaperone StfD, at 54,359 to 55,111
GFF73
Position (kb) 53
54 Strain fitness (log2 ratio) -2
-1
0
1
2 at 52.449 kb on + strand, within GFF69 at 52.449 kb on + strand, within GFF69 at 52.449 kb on + strand, within GFF69 at 52.559 kb on + strand, within GFF69 at 52.560 kb on - strand, within GFF69 at 52.560 kb on - strand, within GFF69 at 52.648 kb on - strand, within GFF69 at 52.648 kb on - strand, within GFF69 at 52.650 kb on + strand, within GFF69 at 52.651 kb on - strand, within GFF69 at 52.714 kb on + strand, within GFF69 at 52.719 kb on + strand, within GFF69 at 52.720 kb on - strand, within GFF69 at 52.720 kb on - strand, within GFF69 at 52.723 kb on - strand, within GFF69 at 52.828 kb on + strand at 52.828 kb on + strand at 53.031 kb on - strand, within GFF70 at 53.034 kb on + strand, within GFF70 at 53.034 kb on + strand, within GFF70 at 53.036 kb on + strand, within GFF70 at 53.146 kb on - strand, within GFF70 at 53.490 kb on - strand, within GFF71 at 53.730 kb on - strand, within GFF71 at 53.835 kb on + strand at 54.257 kb on - strand, within GFF72 at 54.406 kb on + strand at 54.407 kb on - strand at 54.450 kb on + strand, within GFF73 at 54.589 kb on - strand, within GFF73 at 54.589 kb on - strand, within GFF73 at 54.645 kb on + strand, within GFF73 at 54.649 kb on - strand, within GFF73 at 54.649 kb on - strand, within GFF73
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 52,449 + GFF69 0.55 -1.9 52,449 + GFF69 0.55 -0.2 52,449 + GFF69 0.55 -0.2 52,559 + GFF69 0.68 +2.3 52,560 - GFF69 0.68 -0.1 52,560 - GFF69 0.68 +1.2 52,648 - GFF69 0.79 -0.1 52,648 - GFF69 0.79 -0.1 52,650 + GFF69 0.79 -0.6 52,651 - GFF69 0.79 -0.7 52,714 + GFF69 0.87 +2.4 52,719 + GFF69 0.87 +0.2 52,720 - GFF69 0.87 +0.1 52,720 - GFF69 0.87 -0.5 52,723 - GFF69 0.88 -1.6 52,828 + -0.3 52,828 + +1.5 53,031 - GFF70 0.39 -0.2 53,034 + GFF70 0.39 +2.4 53,034 + GFF70 0.39 -0.0 53,036 + GFF70 0.40 -0.2 53,146 - GFF70 0.60 -0.5 53,490 - GFF71 0.28 -0.5 53,730 - GFF71 0.79 -0.9 53,835 + -0.7 54,257 - GFF72 0.84 +0.9 54,406 + +0.9 54,407 - -0.9 54,450 + GFF73 0.12 +1.3 54,589 - GFF73 0.31 -0.5 54,589 - GFF73 0.31 -0.3 54,645 + GFF73 0.38 -1.0 54,649 - GFF73 0.39 +1.8 54,649 - GFF73 0.39 +0.4
Or see this region's nucleotide sequence