Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF615 and GFF616 are separated by 53 nucleotides GFF616 and GFF617 are separated by 191 nucleotides GFF617 and GFF618 are separated by 109 nucleotides
GFF615 - Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49), at 354,897 to 356,516
GFF615
GFF616 - hypothetical protein, at 356,570 to 356,704
GFF616
GFF617 - Putative transport protein, at 356,896 to 358,605
GFF617
GFF618 - 33 kDa chaperonin (Heat shock protein 33) (HSP33), at 358,715 to 359,599
GFF618
Position (kb)
356
357
358
359 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 355.925 kb on + strand, within GFF615 at 356.018 kb on + strand, within GFF615 at 356.240 kb on + strand, within GFF615 at 356.279 kb on + strand, within GFF615 at 356.306 kb on + strand, within GFF615 at 356.306 kb on + strand, within GFF615 at 356.306 kb on + strand, within GFF615 at 356.307 kb on - strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.312 kb on + strand, within GFF615 at 356.370 kb on - strand at 356.373 kb on + strand at 356.373 kb on + strand at 356.390 kb on - strand at 356.408 kb on + strand at 356.423 kb on - strand at 356.434 kb on + strand at 356.476 kb on + strand at 356.477 kb on - strand at 356.538 kb on + strand at 356.539 kb on - strand at 356.539 kb on - strand at 356.539 kb on - strand at 356.540 kb on + strand at 356.593 kb on + strand, within GFF616 at 356.600 kb on - strand, within GFF616 at 356.736 kb on + strand at 356.737 kb on - strand at 356.761 kb on + strand at 356.919 kb on + strand at 356.920 kb on - strand at 356.922 kb on + strand at 356.922 kb on + strand at 356.971 kb on + strand at 356.972 kb on - strand at 356.972 kb on - strand at 357.003 kb on + strand at 357.020 kb on - strand at 357.020 kb on - strand at 357.020 kb on - strand at 357.024 kb on + strand at 357.057 kb on - strand at 357.057 kb on - strand at 357.064 kb on - strand at 357.078 kb on + strand, within GFF617 at 357.202 kb on + strand, within GFF617 at 357.253 kb on + strand, within GFF617 at 357.320 kb on + strand, within GFF617 at 357.320 kb on + strand, within GFF617 at 357.448 kb on - strand, within GFF617 at 357.452 kb on - strand, within GFF617 at 357.591 kb on + strand, within GFF617 at 357.603 kb on - strand, within GFF617 at 357.719 kb on + strand, within GFF617 at 357.741 kb on - strand, within GFF617 at 357.781 kb on + strand, within GFF617 at 357.781 kb on + strand, within GFF617 at 357.782 kb on - strand, within GFF617 at 357.782 kb on - strand, within GFF617 at 357.783 kb on + strand, within GFF617 at 357.783 kb on + strand, within GFF617 at 357.783 kb on + strand, within GFF617 at 357.783 kb on + strand, within GFF617 at 357.783 kb on + strand, within GFF617 at 357.784 kb on - strand, within GFF617 at 357.784 kb on - strand, within GFF617 at 357.826 kb on + strand, within GFF617 at 357.895 kb on + strand, within GFF617 at 357.911 kb on + strand, within GFF617 at 357.953 kb on + strand, within GFF617 at 357.984 kb on - strand, within GFF617 at 358.007 kb on + strand, within GFF617 at 358.023 kb on + strand, within GFF617 at 358.024 kb on - strand, within GFF617 at 358.041 kb on + strand, within GFF617 at 358.057 kb on - strand, within GFF617 at 358.090 kb on + strand, within GFF617 at 358.092 kb on + strand, within GFF617 at 358.159 kb on - strand, within GFF617 at 358.210 kb on - strand, within GFF617 at 358.399 kb on + strand, within GFF617 at 358.400 kb on - strand, within GFF617 at 358.407 kb on + strand, within GFF617 at 358.430 kb on - strand, within GFF617 at 358.436 kb on + strand at 358.491 kb on + strand at 358.529 kb on + strand at 358.557 kb on + strand at 358.786 kb on - strand at 359.027 kb on - strand, within GFF618 at 359.191 kb on + strand, within GFF618 at 359.220 kb on + strand, within GFF618 at 359.243 kb on - strand, within GFF618 at 359.433 kb on - strand, within GFF618 at 359.574 kb on - strand at 359.574 kb on - strand at 359.594 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 355,925 + GFF615 0.63 +0.0 356,018 + GFF615 0.69 -0.2 356,240 + GFF615 0.83 -0.4 356,279 + GFF615 0.85 -0.6 356,306 + GFF615 0.87 +0.3 356,306 + GFF615 0.87 +0.2 356,306 + GFF615 0.87 -0.3 356,307 - GFF615 0.87 +0.1 356,308 + GFF615 0.87 +0.8 356,308 + GFF615 0.87 +0.4 356,308 + GFF615 0.87 +0.1 356,308 + GFF615 0.87 +0.4 356,309 - GFF615 0.87 +0.5 356,309 - GFF615 0.87 -1.4 356,309 - GFF615 0.87 +0.9 356,309 - GFF615 0.87 +0.5 356,312 + GFF615 0.87 +2.8 356,370 - +0.5 356,373 + -0.4 356,373 + -0.1 356,390 - +0.1 356,408 + +0.5 356,423 - -0.0 356,434 + +0.4 356,476 + -0.2 356,477 - -2.4 356,538 + -0.1 356,539 - -0.6 356,539 - -0.3 356,539 - -0.4 356,540 + -0.3 356,593 + GFF616 0.17 -0.1 356,600 - GFF616 0.22 -0.0 356,736 + +0.4 356,737 - -0.5 356,761 + -0.4 356,919 + +0.4 356,920 - -2.9 356,922 + -0.8 356,922 + -0.4 356,971 + -0.4 356,972 - +0.3 356,972 - +0.0 357,003 + +0.4 357,020 - -0.0 357,020 - -0.3 357,020 - +0.6 357,024 + +0.5 357,057 - +0.3 357,057 - +1.4 357,064 - -0.2 357,078 + GFF617 0.11 -0.5 357,202 + GFF617 0.18 +0.8 357,253 + GFF617 0.21 -0.6 357,320 + GFF617 0.25 -0.1 357,320 + GFF617 0.25 +0.7 357,448 - GFF617 0.32 -0.0 357,452 - GFF617 0.33 +0.9 357,591 + GFF617 0.41 +0.7 357,603 - GFF617 0.41 -0.1 357,719 + GFF617 0.48 -0.0 357,741 - GFF617 0.49 +1.0 357,781 + GFF617 0.52 +0.6 357,781 + GFF617 0.52 +1.6 357,782 - GFF617 0.52 +0.0 357,782 - GFF617 0.52 -2.2 357,783 + GFF617 0.52 -0.3 357,783 + GFF617 0.52 +0.5 357,783 + GFF617 0.52 -0.1 357,783 + GFF617 0.52 -0.3 357,783 + GFF617 0.52 +0.8 357,784 - GFF617 0.52 -3.5 357,784 - GFF617 0.52 -0.6 357,826 + GFF617 0.54 -0.2 357,895 + GFF617 0.58 -0.1 357,911 + GFF617 0.59 +0.2 357,953 + GFF617 0.62 +1.5 357,984 - GFF617 0.64 +0.3 358,007 + GFF617 0.65 +1.0 358,023 + GFF617 0.66 -0.3 358,024 - GFF617 0.66 -0.4 358,041 + GFF617 0.67 +0.2 358,057 - GFF617 0.68 -0.2 358,090 + GFF617 0.70 -0.5 358,092 + GFF617 0.70 -0.1 358,159 - GFF617 0.74 -0.7 358,210 - GFF617 0.77 -2.2 358,399 + GFF617 0.88 -0.0 358,400 - GFF617 0.88 +0.5 358,407 + GFF617 0.88 +0.6 358,430 - GFF617 0.90 +1.1 358,436 + -0.2 358,491 + -0.4 358,529 + -0.4 358,557 + -0.2 358,786 - +1.4 359,027 - GFF618 0.35 +1.6 359,191 + GFF618 0.54 +1.4 359,220 + GFF618 0.57 +1.5 359,243 - GFF618 0.60 +1.4 359,433 - GFF618 0.81 +1.7 359,574 - +0.9 359,574 - +1.0 359,594 - +1.1
Or see this region's nucleotide sequence