Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF585 and GFF586 are separated by 9 nucleotides GFF586 and GFF587 are separated by 46 nucleotides GFF587 and GFF588 are separated by 198 nucleotides GFF588 and GFF589 are separated by 75 nucleotides
GFF585 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 320,778 to 322,238
GFF585
GFF586 - Putative inner membrane protein, at 322,248 to 322,793
GFF586
GFF587 - Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), at 322,840 to 324,348
GFF587
GFF588 - Thiosulfate sulfurtransferase GlpE (EC 2.8.1.1), at 324,547 to 324,873
GFF588
GFF589 - GlpG protein (membrane protein of glp regulon), at 324,949 to 325,779
GFF589
Position (kb) 322
323
324
325 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 321.877 kb on + strand, within GFF585 at 322.002 kb on + strand, within GFF585 at 322.002 kb on + strand, within GFF585 at 322.034 kb on + strand, within GFF585 at 322.034 kb on + strand, within GFF585 at 322.092 kb on + strand, within GFF585 at 322.099 kb on - strand at 322.114 kb on - strand at 322.115 kb on + strand at 322.116 kb on - strand at 322.209 kb on + strand at 322.210 kb on - strand at 322.210 kb on - strand at 322.343 kb on + strand, within GFF586 at 322.349 kb on + strand, within GFF586 at 322.349 kb on + strand, within GFF586 at 322.438 kb on + strand, within GFF586 at 322.642 kb on - strand, within GFF586 at 322.670 kb on + strand, within GFF586 at 322.733 kb on - strand, within GFF586 at 322.751 kb on + strand at 322.752 kb on - strand at 322.763 kb on + strand at 322.763 kb on + strand at 322.800 kb on + strand at 322.801 kb on - strand at 322.801 kb on - strand at 322.822 kb on - strand at 322.842 kb on - strand at 322.950 kb on + strand at 323.100 kb on - strand, within GFF587 at 323.463 kb on - strand, within GFF587 at 323.524 kb on - strand, within GFF587 at 323.525 kb on + strand, within GFF587 at 323.784 kb on + strand, within GFF587 at 323.865 kb on + strand, within GFF587 at 324.027 kb on - strand, within GFF587 at 324.191 kb on - strand, within GFF587 at 324.451 kb on + strand at 324.452 kb on - strand at 324.455 kb on + strand at 324.455 kb on + strand at 324.456 kb on - strand at 324.456 kb on - strand at 324.457 kb on + strand at 324.476 kb on + strand at 324.489 kb on + strand at 324.490 kb on - strand at 324.512 kb on - strand at 324.564 kb on + strand at 324.564 kb on + strand at 324.564 kb on + strand at 324.565 kb on - strand at 324.584 kb on - strand, within GFF588 at 324.735 kb on - strand, within GFF588 at 324.743 kb on - strand, within GFF588 at 324.756 kb on + strand, within GFF588 at 324.776 kb on - strand, within GFF588 at 324.793 kb on + strand, within GFF588 at 324.909 kb on - strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.910 kb on + strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.911 kb on - strand at 324.912 kb on + strand at 324.912 kb on + strand at 324.913 kb on - strand at 324.913 kb on - strand at 324.913 kb on - strand at 324.913 kb on - strand at 324.927 kb on + strand at 325.004 kb on - strand at 325.029 kb on + strand at 325.056 kb on + strand, within GFF589 at 325.270 kb on + strand, within GFF589 at 325.270 kb on + strand, within GFF589 at 325.271 kb on - strand, within GFF589
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 321,877 + GFF585 0.75 -0.7 322,002 + GFF585 0.84 -0.0 322,002 + GFF585 0.84 +0.1 322,034 + GFF585 0.86 -1.1 322,034 + GFF585 0.86 +0.2 322,092 + GFF585 0.90 -0.8 322,099 - -0.2 322,114 - +0.0 322,115 + +0.9 322,116 - -0.0 322,209 + -1.9 322,210 - -0.4 322,210 - -0.0 322,343 + GFF586 0.17 -0.2 322,349 + GFF586 0.18 +0.1 322,349 + GFF586 0.18 -2.2 322,438 + GFF586 0.35 +0.5 322,642 - GFF586 0.72 +1.1 322,670 + GFF586 0.77 -1.7 322,733 - GFF586 0.89 -0.0 322,751 + -0.4 322,752 - +0.3 322,763 + -1.0 322,763 + -0.1 322,800 + -0.4 322,801 - +0.3 322,801 - +1.0 322,822 - +0.6 322,842 - +0.5 322,950 + -0.4 323,100 - GFF587 0.17 -1.2 323,463 - GFF587 0.41 -1.2 323,524 - GFF587 0.45 -0.4 323,525 + GFF587 0.45 -0.3 323,784 + GFF587 0.63 -0.4 323,865 + GFF587 0.68 -2.9 324,027 - GFF587 0.79 -1.6 324,191 - GFF587 0.90 -0.5 324,451 + -0.4 324,452 - +1.9 324,455 + +0.1 324,455 + +0.5 324,456 - -0.4 324,456 - +0.2 324,457 + +2.5 324,476 + -0.0 324,489 + -0.3 324,490 - +0.7 324,512 - -0.2 324,564 + -0.1 324,564 + +0.2 324,564 + -0.6 324,565 - +1.1 324,584 - GFF588 0.11 -0.3 324,735 - GFF588 0.57 -0.1 324,743 - GFF588 0.60 -0.5 324,756 + GFF588 0.64 -0.4 324,776 - GFF588 0.70 -0.4 324,793 + GFF588 0.75 -0.9 324,909 - +0.2 324,910 + -0.6 324,910 + -0.2 324,910 + -0.3 324,910 + -0.7 324,910 + +0.4 324,910 + +2.4 324,910 + +0.6 324,910 + -1.3 324,910 + -0.2 324,911 - -1.2 324,911 - -0.2 324,911 - -0.3 324,911 - +0.6 324,911 - -0.3 324,911 - -0.1 324,911 - -0.4 324,911 - -0.2 324,911 - -0.5 324,912 + -0.6 324,912 + +0.2 324,913 - +0.5 324,913 - -0.2 324,913 - +0.1 324,913 - +0.1 324,927 + -0.7 325,004 - +0.1 325,029 + +1.2 325,056 + GFF589 0.13 +0.1 325,270 + GFF589 0.39 +0.5 325,270 + GFF589 0.39 +1.1 325,271 - GFF589 0.39 +0.3
Or see this region's nucleotide sequence