Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF559 and GFF560 are separated by 12 nucleotides GFF560 and GFF561 are separated by 125 nucleotides GFF561 and GFF562 are separated by 44 nucleotides
GFF559 - Glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46), at 289,579 to 290,319
GFF559
GFF560 - FIG00638953: hypothetical protein, at 290,332 to 290,748
GFF560
GFF561 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 290,874 to 292,616
GFF561
GFF562 - Puative phophotriesterase, at 292,661 to 293,695
GFF562
Position (kb) 290
291
292
293 Strain fitness (log2 ratio) -2
-1
0
1 at 289.914 kb on + strand, within GFF559 at 290.027 kb on + strand, within GFF559 at 290.271 kb on + strand at 290.318 kb on - strand at 290.520 kb on + strand, within GFF560 at 290.671 kb on - strand, within GFF560 at 290.677 kb on - strand, within GFF560 at 290.677 kb on - strand, within GFF560 at 290.688 kb on + strand, within GFF560 at 290.689 kb on - strand, within GFF560 at 290.735 kb on - strand at 290.735 kb on - strand at 290.784 kb on + strand at 290.784 kb on + strand at 290.785 kb on - strand at 290.785 kb on - strand at 290.785 kb on - strand at 290.835 kb on - strand at 290.845 kb on + strand at 290.845 kb on + strand at 290.845 kb on + strand at 290.845 kb on + strand at 290.846 kb on - strand at 290.846 kb on - strand at 290.846 kb on - strand at 290.846 kb on - strand at 291.066 kb on - strand, within GFF561 at 291.167 kb on + strand, within GFF561 at 291.167 kb on + strand, within GFF561 at 291.168 kb on - strand, within GFF561 at 291.245 kb on + strand, within GFF561 at 291.355 kb on - strand, within GFF561 at 291.534 kb on - strand, within GFF561 at 291.625 kb on + strand, within GFF561 at 291.681 kb on + strand, within GFF561 at 291.682 kb on - strand, within GFF561 at 291.789 kb on - strand, within GFF561 at 291.891 kb on + strand, within GFF561 at 291.891 kb on + strand, within GFF561 at 291.892 kb on - strand, within GFF561 at 292.068 kb on - strand, within GFF561 at 292.095 kb on + strand, within GFF561 at 292.274 kb on + strand, within GFF561 at 292.283 kb on + strand, within GFF561 at 292.541 kb on + strand at 292.652 kb on - strand at 292.662 kb on + strand at 292.687 kb on + strand at 292.694 kb on - strand at 292.712 kb on - strand at 292.741 kb on - strand at 292.823 kb on + strand, within GFF562 at 292.953 kb on - strand, within GFF562 at 292.972 kb on + strand, within GFF562 at 293.104 kb on + strand, within GFF562 at 293.129 kb on - strand, within GFF562 at 293.129 kb on - strand, within GFF562 at 293.162 kb on - strand, within GFF562 at 293.254 kb on + strand, within GFF562 at 293.287 kb on + strand, within GFF562 at 293.288 kb on - strand, within GFF562 at 293.319 kb on + strand, within GFF562 at 293.443 kb on + strand, within GFF562 at 293.580 kb on + strand, within GFF562 at 293.580 kb on + strand, within GFF562
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 289,914 + GFF559 0.45 -0.9 290,027 + GFF559 0.60 -0.1 290,271 + +1.6 290,318 - +0.3 290,520 + GFF560 0.45 +0.6 290,671 - GFF560 0.81 +0.3 290,677 - GFF560 0.83 +0.4 290,677 - GFF560 0.83 +0.3 290,688 + GFF560 0.85 +0.4 290,689 - GFF560 0.86 +0.7 290,735 - +0.4 290,735 - +0.4 290,784 + -0.5 290,784 + +0.5 290,785 - +0.3 290,785 - +0.4 290,785 - -0.0 290,835 - -0.1 290,845 + +0.5 290,845 + -0.5 290,845 + -0.2 290,845 + -0.4 290,846 - -0.2 290,846 - +0.1 290,846 - -0.0 290,846 - -0.7 291,066 - GFF561 0.11 -0.3 291,167 + GFF561 0.17 -0.1 291,167 + GFF561 0.17 -0.0 291,168 - GFF561 0.17 +0.3 291,245 + GFF561 0.21 -0.8 291,355 - GFF561 0.28 -0.0 291,534 - GFF561 0.38 -1.0 291,625 + GFF561 0.43 -0.4 291,681 + GFF561 0.46 -0.9 291,682 - GFF561 0.46 -0.3 291,789 - GFF561 0.52 +0.6 291,891 + GFF561 0.58 +0.2 291,891 + GFF561 0.58 -1.8 291,892 - GFF561 0.58 -0.9 292,068 - GFF561 0.69 -0.2 292,095 + GFF561 0.70 -1.1 292,274 + GFF561 0.80 -0.2 292,283 + GFF561 0.81 +0.7 292,541 + +0.3 292,652 - +0.0 292,662 + +0.6 292,687 + -0.5 292,694 - -0.3 292,712 - +0.6 292,741 - +0.6 292,823 + GFF562 0.16 -0.0 292,953 - GFF562 0.28 -0.8 292,972 + GFF562 0.30 -1.5 293,104 + GFF562 0.43 -0.0 293,129 - GFF562 0.45 -0.3 293,129 - GFF562 0.45 -0.3 293,162 - GFF562 0.48 -0.5 293,254 + GFF562 0.57 +0.8 293,287 + GFF562 0.60 +0.2 293,288 - GFF562 0.61 -0.1 293,319 + GFF562 0.64 +0.8 293,443 + GFF562 0.76 +0.1 293,580 + GFF562 0.89 +0.1 293,580 + GFF562 0.89 +0.3
Or see this region's nucleotide sequence