Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4892 and GFF4893 are separated by 545 nucleotides GFF4893 and GFF4894 are separated by 77 nucleotides
GFF4892 - Thioredoxin reductase (EC 1.8.1.9), at 9,550 to 10,518
GFF4892
GFF4893 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 11,064 to 11,558
GFF4893
GFF4894 - Cell division protein FtsK, at 11,636 to 15,670
GFF4894
Position (kb)
11
12 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 10.072 kb on + strand, within GFF4892 at 10.163 kb on + strand, within GFF4892 at 10.417 kb on - strand, within GFF4892 at 10.448 kb on + strand at 10.530 kb on - strand at 10.575 kb on + strand at 10.612 kb on + strand at 10.615 kb on + strand at 10.615 kb on + strand at 10.615 kb on + strand at 10.813 kb on + strand at 10.828 kb on + strand at 10.840 kb on + strand at 10.871 kb on + strand at 11.020 kb on - strand at 11.035 kb on + strand at 11.060 kb on - strand at 11.060 kb on - strand at 11.062 kb on + strand at 11.062 kb on + strand at 11.063 kb on - strand at 11.069 kb on - strand at 11.072 kb on + strand at 11.123 kb on + strand, within GFF4893 at 11.123 kb on + strand, within GFF4893 at 11.147 kb on + strand, within GFF4893 at 11.147 kb on + strand, within GFF4893 at 11.147 kb on + strand, within GFF4893 at 11.168 kb on + strand, within GFF4893 at 11.234 kb on + strand, within GFF4893 at 11.268 kb on + strand, within GFF4893 at 11.343 kb on - strand, within GFF4893 at 11.406 kb on + strand, within GFF4893 at 11.426 kb on - strand, within GFF4893 at 11.502 kb on + strand, within GFF4893 at 11.506 kb on + strand, within GFF4893 at 11.546 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 10,072 + GFF4892 0.54 +5.1 10,163 + GFF4892 0.63 +2.3 10,417 - GFF4892 0.89 +3.3 10,448 + +1.8 10,530 - +0.8 10,575 + +1.1 10,612 + -0.0 10,615 + -1.9 10,615 + +2.2 10,615 + +1.8 10,813 + +1.9 10,828 + +1.4 10,840 + +1.1 10,871 + +0.5 11,020 - +0.1 11,035 + +0.7 11,060 - +1.2 11,060 - +0.6 11,062 + +0.6 11,062 + +0.9 11,063 - +1.1 11,069 - -1.2 11,072 + +0.2 11,123 + GFF4893 0.12 +0.4 11,123 + GFF4893 0.12 +1.6 11,147 + GFF4893 0.17 +0.7 11,147 + GFF4893 0.17 -0.0 11,147 + GFF4893 0.17 -0.2 11,168 + GFF4893 0.21 -0.2 11,234 + GFF4893 0.34 -0.5 11,268 + GFF4893 0.41 -0.5 11,343 - GFF4893 0.56 +0.7 11,406 + GFF4893 0.69 +0.6 11,426 - GFF4893 0.73 +1.2 11,502 + GFF4893 0.88 +0.2 11,506 + GFF4893 0.89 +1.4 11,546 + +0.0
Or see this region's nucleotide sequence