Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4863 and GFF4864 overlap by 8 nucleotides GFF4864 and GFF4865 are separated by 18 nucleotides
GFF4863 - 'LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM' transl_table=11, at 162,730 to 163,830
GFF4863
GFF4864 - COG2363, at 163,823 to 164,218
GFF4864
GFF4865 - Glycine cleavage system transcriptional activator GcvA, at 164,237 to 165,154
GFF4865
Position (kb) 163
164
165 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 162.866 kb on + strand, within GFF4863 at 163.115 kb on - strand, within GFF4863 at 163.115 kb on - strand, within GFF4863 at 163.131 kb on + strand, within GFF4863 at 163.132 kb on - strand, within GFF4863 at 163.190 kb on + strand, within GFF4863 at 163.190 kb on + strand, within GFF4863 at 163.191 kb on - strand, within GFF4863 at 163.191 kb on - strand, within GFF4863 at 163.191 kb on - strand, within GFF4863 at 163.191 kb on - strand, within GFF4863 at 163.191 kb on - strand, within GFF4863 at 163.314 kb on + strand, within GFF4863 at 163.356 kb on - strand, within GFF4863 at 163.356 kb on - strand, within GFF4863 at 163.492 kb on - strand, within GFF4863 at 163.691 kb on + strand, within GFF4863 at 163.701 kb on - strand, within GFF4863 at 163.817 kb on - strand at 163.824 kb on + strand at 163.824 kb on + strand at 163.844 kb on - strand at 163.852 kb on + strand at 164.173 kb on + strand, within GFF4864 at 164.174 kb on - strand, within GFF4864 at 164.203 kb on + strand at 164.225 kb on - strand at 164.229 kb on + strand at 164.352 kb on + strand, within GFF4865 at 164.429 kb on - strand, within GFF4865 at 164.435 kb on - strand, within GFF4865 at 164.495 kb on - strand, within GFF4865 at 164.495 kb on - strand, within GFF4865 at 164.499 kb on - strand, within GFF4865 at 164.518 kb on + strand, within GFF4865 at 164.521 kb on + strand, within GFF4865 at 164.545 kb on + strand, within GFF4865 at 164.547 kb on + strand, within GFF4865 at 164.548 kb on - strand, within GFF4865 at 164.582 kb on + strand, within GFF4865 at 164.661 kb on - strand, within GFF4865 at 164.807 kb on - strand, within GFF4865 at 164.899 kb on + strand, within GFF4865 at 164.936 kb on - strand, within GFF4865
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 162,866 + GFF4863 0.12 +0.7 163,115 - GFF4863 0.35 -0.1 163,115 - GFF4863 0.35 +0.7 163,131 + GFF4863 0.36 +1.0 163,132 - GFF4863 0.37 +0.6 163,190 + GFF4863 0.42 +1.5 163,190 + GFF4863 0.42 +0.3 163,191 - GFF4863 0.42 -0.7 163,191 - GFF4863 0.42 -3.2 163,191 - GFF4863 0.42 +0.7 163,191 - GFF4863 0.42 -2.9 163,191 - GFF4863 0.42 +0.0 163,314 + GFF4863 0.53 +1.2 163,356 - GFF4863 0.57 -1.2 163,356 - GFF4863 0.57 -0.1 163,492 - GFF4863 0.69 +1.0 163,691 + GFF4863 0.87 +0.6 163,701 - GFF4863 0.88 +1.9 163,817 - -0.3 163,824 + -0.1 163,824 + +0.9 163,844 - -1.2 163,852 + +1.9 164,173 + GFF4864 0.88 +0.4 164,174 - GFF4864 0.89 +1.7 164,203 + +0.5 164,225 - +1.5 164,229 + +0.8 164,352 + GFF4865 0.13 -0.1 164,429 - GFF4865 0.21 -0.6 164,435 - GFF4865 0.22 -0.5 164,495 - GFF4865 0.28 -0.3 164,495 - GFF4865 0.28 +2.5 164,499 - GFF4865 0.29 -0.0 164,518 + GFF4865 0.31 +0.8 164,521 + GFF4865 0.31 +0.5 164,545 + GFF4865 0.34 -0.3 164,547 + GFF4865 0.34 -0.2 164,548 - GFF4865 0.34 -1.0 164,582 + GFF4865 0.38 +1.0 164,661 - GFF4865 0.46 -1.9 164,807 - GFF4865 0.62 -1.9 164,899 + GFF4865 0.72 +0.5 164,936 - GFF4865 0.76 -2.3
Or see this region's nucleotide sequence