Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4819 and GFF4820 overlap by 1 nucleotides GFF4820 and GFF4821 are separated by 324 nucleotides GFF4821 and GFF4822 are separated by 30 nucleotides GFF4822 and GFF4823 are separated by 87 nucleotides
GFF4819 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 118,965 to 120,677
GFF4819
GFF4820 - Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2), at 120,677 to 122,476
GFF4820
GFF4821 - FIG01047384: hypothetical protein, at 122,801 to 122,971
GFF4821
GFF4822 - Queuosine biosynthesis QueD, PTPS-I, at 123,002 to 123,262
GFF4822
GFF4823 - STY3078 protein, at 123,350 to 124,147
GFF4823
Position (kb)
120
121
122
123 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 119.721 kb on - strand, within GFF4819 at 119.892 kb on - strand, within GFF4819 at 119.892 kb on - strand, within GFF4819 at 120.167 kb on - strand, within GFF4819 at 120.194 kb on - strand, within GFF4819 at 120.206 kb on + strand, within GFF4819 at 120.219 kb on - strand, within GFF4819 at 120.505 kb on + strand, within GFF4819 at 120.599 kb on - strand at 120.638 kb on + strand at 120.707 kb on + strand at 120.722 kb on + strand at 120.828 kb on + strand at 120.829 kb on - strand at 120.829 kb on - strand at 120.832 kb on + strand at 120.897 kb on + strand, within GFF4820 at 120.964 kb on - strand, within GFF4820 at 121.033 kb on + strand, within GFF4820 at 121.270 kb on + strand, within GFF4820 at 121.315 kb on - strand, within GFF4820 at 121.416 kb on + strand, within GFF4820 at 121.445 kb on + strand, within GFF4820 at 121.780 kb on + strand, within GFF4820 at 121.784 kb on - strand, within GFF4820 at 121.784 kb on - strand, within GFF4820 at 121.786 kb on - strand, within GFF4820 at 121.786 kb on - strand, within GFF4820 at 122.278 kb on + strand, within GFF4820 at 122.278 kb on + strand, within GFF4820 at 122.484 kb on + strand at 122.561 kb on - strand at 122.561 kb on - strand at 122.917 kb on + strand, within GFF4821 at 122.921 kb on + strand, within GFF4821 at 122.972 kb on - strand at 123.092 kb on + strand, within GFF4822 at 123.114 kb on - strand, within GFF4822
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 119,721 - GFF4819 0.44 +0.2 119,892 - GFF4819 0.54 +0.4 119,892 - GFF4819 0.54 +0.4 120,167 - GFF4819 0.70 +1.2 120,194 - GFF4819 0.72 -2.5 120,206 + GFF4819 0.72 -2.1 120,219 - GFF4819 0.73 +0.9 120,505 + GFF4819 0.90 -0.9 120,599 - -0.7 120,638 + +2.1 120,707 + -0.1 120,722 + +0.1 120,828 + -0.6 120,829 - +0.5 120,829 - -0.6 120,832 + -0.2 120,897 + GFF4820 0.12 +0.1 120,964 - GFF4820 0.16 +0.4 121,033 + GFF4820 0.20 +0.9 121,270 + GFF4820 0.33 +0.3 121,315 - GFF4820 0.35 +0.9 121,416 + GFF4820 0.41 +1.4 121,445 + GFF4820 0.43 -0.3 121,780 + GFF4820 0.61 -0.7 121,784 - GFF4820 0.61 +0.3 121,784 - GFF4820 0.61 +0.9 121,786 - GFF4820 0.62 +0.5 121,786 - GFF4820 0.62 +1.1 122,278 + GFF4820 0.89 +0.7 122,278 + GFF4820 0.89 -2.5 122,484 + +1.2 122,561 - -0.1 122,561 - -0.7 122,917 + GFF4821 0.68 +0.9 122,921 + GFF4821 0.70 -0.1 122,972 - +0.2 123,092 + GFF4822 0.34 +0.9 123,114 - GFF4822 0.43 +0.9
Or see this region's nucleotide sequence