Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4791 and GFF4792 overlap by 7 nucleotides GFF4792 and GFF4793 overlap by 11 nucleotides GFF4793 and GFF4794 overlap by 4 nucleotides GFF4794 and GFF4795 overlap by 1 nucleotides
GFF4791 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 97,641 to 98,267
GFF4791
GFF4792 - 5-nucleotidase SurE (EC 3.1.3.5) @ Exopolyphosphatase (EC 3.6.1.11), at 98,261 to 99,013
GFF4792
GFF4793 - tRNA pseudouridine 13 synthase (EC 4.2.1.-), at 99,003 to 100,052
GFF4793
GFF4794 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 100,049 to 100,528
GFF4794
GFF4795 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60), at 100,528 to 101,238
GFF4795
Position (kb) 99
100
101 Strain fitness (log2 ratio) -1
0
1
2 at 98.081 kb on + strand, within GFF4791 at 98.082 kb on - strand, within GFF4791 at 98.083 kb on + strand, within GFF4791 at 98.083 kb on + strand, within GFF4791 at 98.084 kb on - strand, within GFF4791 at 98.084 kb on - strand, within GFF4791 at 98.127 kb on - strand, within GFF4791 at 98.142 kb on - strand, within GFF4791 at 98.162 kb on + strand, within GFF4791 at 98.250 kb on + strand at 98.251 kb on - strand at 98.407 kb on - strand, within GFF4792 at 98.440 kb on + strand, within GFF4792 at 98.440 kb on + strand, within GFF4792 at 98.440 kb on + strand, within GFF4792 at 98.440 kb on + strand, within GFF4792 at 98.440 kb on + strand, within GFF4792 at 98.441 kb on - strand, within GFF4792 at 98.469 kb on - strand, within GFF4792 at 98.535 kb on - strand, within GFF4792 at 98.535 kb on - strand, within GFF4792 at 98.631 kb on + strand, within GFF4792 at 98.631 kb on + strand, within GFF4792 at 98.787 kb on - strand, within GFF4792 at 98.835 kb on - strand, within GFF4792 at 99.015 kb on - strand at 99.094 kb on - strand at 99.129 kb on + strand, within GFF4793 at 99.130 kb on - strand, within GFF4793 at 99.287 kb on + strand, within GFF4793 at 99.660 kb on - strand, within GFF4793 at 99.695 kb on + strand, within GFF4793 at 99.695 kb on + strand, within GFF4793 at 99.696 kb on - strand, within GFF4793 at 99.713 kb on - strand, within GFF4793
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 98,081 + GFF4791 0.70 -0.0 98,082 - GFF4791 0.70 -0.9 98,083 + GFF4791 0.70 +1.3 98,083 + GFF4791 0.70 -0.8 98,084 - GFF4791 0.71 -0.6 98,084 - GFF4791 0.71 +2.5 98,127 - GFF4791 0.78 -0.1 98,142 - GFF4791 0.80 -0.7 98,162 + GFF4791 0.83 +0.3 98,250 + -0.3 98,251 - +0.5 98,407 - GFF4792 0.19 -0.6 98,440 + GFF4792 0.24 -0.3 98,440 + GFF4792 0.24 -0.1 98,440 + GFF4792 0.24 +2.5 98,440 + GFF4792 0.24 +0.1 98,440 + GFF4792 0.24 +0.7 98,441 - GFF4792 0.24 -1.2 98,469 - GFF4792 0.28 -0.8 98,535 - GFF4792 0.36 +0.1 98,535 - GFF4792 0.36 +0.2 98,631 + GFF4792 0.49 -0.2 98,631 + GFF4792 0.49 -1.3 98,787 - GFF4792 0.70 +0.1 98,835 - GFF4792 0.76 -0.9 99,015 - +0.3 99,094 - +0.6 99,129 + GFF4793 0.12 +0.1 99,130 - GFF4793 0.12 -1.6 99,287 + GFF4793 0.27 -0.9 99,660 - GFF4793 0.63 +0.6 99,695 + GFF4793 0.66 -0.2 99,695 + GFF4793 0.66 -0.3 99,696 - GFF4793 0.66 -0.2 99,713 - GFF4793 0.68 -0.5
Or see this region's nucleotide sequence