Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4712

Experiment: LB_plus_SM_buffer with S16_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4709 and GFF4710 are separated by 26 nucleotidesGFF4710 and GFF4711 overlap by 8 nucleotidesGFF4711 and GFF4712 overlap by 4 nucleotidesGFF4712 and GFF4713 overlap by 1 nucleotidesGFF4713 and GFF4714 are separated by 9 nucleotides GFF4709 - Putative periplasmic or exported protein, at 20,649 to 21,458 GFF4709 GFF4710 - Coenzyme F420 hydrogenase maturation protease (EC 3.4.24.-), at 21,485 to 21,955 GFF4710 GFF4711 - Formate hydrogenlyase maturation protein HycH, at 21,948 to 22,358 GFF4711 GFF4712 - Formate hydrogenlyase subunit 7, at 22,355 to 23,122 GFF4712 GFF4713 - Formate hydrogenlyase complex 3 iron-sulfur protein; Formate hydrogenlyase subunit 6; Ni,Fe-hydrogenase III medium subunit, at 23,122 to 23,664 GFF4713 GFF4714 - Formate hydrogenlyase subunit 5, at 23,674 to 25,383 GFF4714 Position (kb) 22 23 24Strain fitness (log2 ratio) -1 0 1 2at 21.400 kb on - strandat 21.437 kb on + strandat 21.438 kb on - strandat 21.474 kb on - strandat 21.474 kb on - strandat 21.550 kb on - strand, within GFF4710at 21.665 kb on - strand, within GFF4710at 21.670 kb on - strand, within GFF4710at 21.670 kb on - strand, within GFF4710at 21.670 kb on - strand, within GFF4710at 21.670 kb on - strand, within GFF4710at 21.670 kb on - strand, within GFF4710at 21.682 kb on + strand, within GFF4710at 21.752 kb on - strand, within GFF4710at 21.942 kb on - strandat 22.004 kb on - strand, within GFF4711at 22.010 kb on + strand, within GFF4711at 22.124 kb on - strand, within GFF4711at 22.193 kb on - strand, within GFF4711at 22.316 kb on + strand, within GFF4711at 22.480 kb on - strand, within GFF4712at 22.480 kb on - strand, within GFF4712at 22.701 kb on + strand, within GFF4712at 22.701 kb on + strand, within GFF4712at 22.701 kb on + strand, within GFF4712at 22.702 kb on - strand, within GFF4712at 22.796 kb on - strand, within GFF4712at 22.978 kb on + strand, within GFF4712at 22.979 kb on - strand, within GFF4712at 23.191 kb on - strand, within GFF4713at 23.270 kb on + strand, within GFF4713at 23.379 kb on - strand, within GFF4713at 23.439 kb on + strand, within GFF4713at 23.439 kb on + strand, within GFF4713at 23.658 kb on + strandat 23.675 kb on + strandat 23.789 kb on + strandat 23.850 kb on + strand, within GFF4714at 23.850 kb on + strand, within GFF4714at 23.851 kb on - strand, within GFF4714at 23.852 kb on + strand, within GFF4714at 23.853 kb on - strand, within GFF4714at 23.997 kb on + strand, within GFF4714at 23.997 kb on + strand, within GFF4714at 23.998 kb on - strand, within GFF4714at 24.034 kb on - strand, within GFF4714at 24.068 kb on + strand, within GFF4714at 24.068 kb on + strand, within GFF4714at 24.069 kb on - strand, within GFF4714

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI
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21,400 - +0.3
21,437 + +0.7
21,438 - +0.0
21,474 - +0.3
21,474 - +1.1
21,550 - GFF4710 0.14 -1.3
21,665 - GFF4710 0.38 +0.1
21,670 - GFF4710 0.39 +0.9
21,670 - GFF4710 0.39 +0.3
21,670 - GFF4710 0.39 +0.3
21,670 - GFF4710 0.39 +1.0
21,670 - GFF4710 0.39 +0.2
21,682 + GFF4710 0.42 -1.3
21,752 - GFF4710 0.57 +0.3
21,942 - +0.7
22,004 - GFF4711 0.14 -0.5
22,010 + GFF4711 0.15 -0.0
22,124 - GFF4711 0.43 +0.2
22,193 - GFF4711 0.60 +1.2
22,316 + GFF4711 0.90 -0.4
22,480 - GFF4712 0.16 +0.5
22,480 - GFF4712 0.16 -0.1
22,701 + GFF4712 0.45 +2.0
22,701 + GFF4712 0.45 +0.0
22,701 + GFF4712 0.45 -0.5
22,702 - GFF4712 0.45 +0.9
22,796 - GFF4712 0.57 -0.3
22,978 + GFF4712 0.81 -1.1
22,979 - GFF4712 0.81 -0.6
23,191 - GFF4713 0.13 -1.1
23,270 + GFF4713 0.27 +0.7
23,379 - GFF4713 0.47 +0.6
23,439 + GFF4713 0.58 +0.3
23,439 + GFF4713 0.58 +1.7
23,658 + +0.2
23,675 + +0.5
23,789 + +0.2
23,850 + GFF4714 0.10 +0.1
23,850 + GFF4714 0.10 -1.0
23,851 - GFF4714 0.10 +0.2
23,852 + GFF4714 0.10 +0.2
23,853 - GFF4714 0.10 +0.1
23,997 + GFF4714 0.19 +0.6
23,997 + GFF4714 0.19 +1.2
23,998 - GFF4714 0.19 -0.1
24,034 - GFF4714 0.21 -0.7
24,068 + GFF4714 0.23 -0.3
24,068 + GFF4714 0.23 +0.8
24,069 - GFF4714 0.23 +0.6

Or see this region's nucleotide sequence