Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF465 and GFF466 are separated by 84 nucleotides GFF466 and GFF467 are separated by 22 nucleotides
GFF465 - Long polar fimbria protein A precursor, at 185,105 to 185,629
GFF465
GFF466 - Chaperone protein lpfB precursor, at 185,714 to 186,412
GFF466
GFF467 - type 1 fimbriae anchoring protein FimD, at 186,435 to 188,963
GFF467
Position (kb) 185
186
187 Strain fitness (log2 ratio) -1
0
1
2 at 185.082 kb on + strand at 185.473 kb on + strand, within GFF465 at 185.500 kb on + strand, within GFF465 at 185.512 kb on + strand, within GFF465 at 185.543 kb on + strand, within GFF465 at 185.544 kb on - strand, within GFF465 at 185.555 kb on + strand, within GFF465 at 185.556 kb on - strand, within GFF465 at 185.572 kb on + strand, within GFF465 at 185.577 kb on + strand at 185.577 kb on + strand at 185.657 kb on + strand at 185.813 kb on - strand, within GFF466 at 185.846 kb on - strand, within GFF466 at 186.038 kb on - strand, within GFF466 at 186.361 kb on + strand at 186.598 kb on - strand at 186.598 kb on - strand at 186.629 kb on + strand at 187.011 kb on + strand, within GFF467 at 187.012 kb on - strand, within GFF467 at 187.053 kb on + strand, within GFF467 at 187.056 kb on + strand, within GFF467 at 187.099 kb on + strand, within GFF467 at 187.100 kb on - strand, within GFF467 at 187.103 kb on + strand, within GFF467 at 187.103 kb on + strand, within GFF467 at 187.104 kb on - strand, within GFF467 at 187.104 kb on - strand, within GFF467 at 187.107 kb on + strand, within GFF467 at 187.108 kb on - strand, within GFF467 at 187.108 kb on - strand, within GFF467 at 187.109 kb on + strand, within GFF467 at 187.181 kb on - strand, within GFF467 at 187.264 kb on + strand, within GFF467 at 187.369 kb on - strand, within GFF467 at 187.383 kb on - strand, within GFF467
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 185,082 + +1.0 185,473 + GFF465 0.70 -1.4 185,500 + GFF465 0.75 +0.3 185,512 + GFF465 0.78 -0.1 185,543 + GFF465 0.83 +0.5 185,544 - GFF465 0.84 -0.5 185,555 + GFF465 0.86 -0.2 185,556 - GFF465 0.86 +0.4 185,572 + GFF465 0.89 +0.6 185,577 + -0.7 185,577 + -0.6 185,657 + +2.3 185,813 - GFF466 0.14 -0.4 185,846 - GFF466 0.19 -0.4 186,038 - GFF466 0.46 -0.9 186,361 + +0.1 186,598 - -0.7 186,598 - -0.0 186,629 + -0.4 187,011 + GFF467 0.23 -0.6 187,012 - GFF467 0.23 +0.1 187,053 + GFF467 0.24 -0.5 187,056 + GFF467 0.25 -1.1 187,099 + GFF467 0.26 +0.8 187,100 - GFF467 0.26 -0.2 187,103 + GFF467 0.26 +0.0 187,103 + GFF467 0.26 +0.8 187,104 - GFF467 0.26 +0.5 187,104 - GFF467 0.26 +0.2 187,107 + GFF467 0.27 +0.0 187,108 - GFF467 0.27 -0.4 187,108 - GFF467 0.27 -0.2 187,109 + GFF467 0.27 -0.8 187,181 - GFF467 0.29 -0.4 187,264 + GFF467 0.33 +0.1 187,369 - GFF467 0.37 +2.0 187,383 - GFF467 0.37 -0.5
Or see this region's nucleotide sequence