Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4547

Experiment: LB_plus_SM_buffer with S16_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4546 and GFF4547 are separated by 10 nucleotidesGFF4547 and GFF4548 are separated by 2 nucleotides GFF4546 - Rare lipoprotein A precursor, at 329,966 to 331,111 GFF4546 GFF4547 - Rod shape-determining protein RodA, at 331,122 to 332,234 GFF4547 GFF4548 - Penicillin-binding protein 2 (PBP-2), at 332,237 to 334,138 GFF4548 Position (kb) 331 332 333Strain fitness (log2 ratio) -2 -1 0 1at 330.122 kb on + strand, within GFF4546at 330.439 kb on + strand, within GFF4546at 330.507 kb on - strand, within GFF4546at 330.615 kb on - strand, within GFF4546at 330.619 kb on + strand, within GFF4546at 330.625 kb on + strand, within GFF4546at 330.753 kb on + strand, within GFF4546at 330.850 kb on - strand, within GFF4546at 331.001 kb on + strandat 331.002 kb on - strandat 331.048 kb on + strandat 331.051 kb on + strandat 331.052 kb on - strandat 331.052 kb on - strandat 331.064 kb on + strandat 331.064 kb on + strandat 331.068 kb on - strandat 331.068 kb on - strandat 331.070 kb on + strandat 331.071 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI
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330,122 + GFF4546 0.14 +0.4
330,439 + GFF4546 0.41 -0.8
330,507 - GFF4546 0.47 +0.3
330,615 - GFF4546 0.57 +1.0
330,619 + GFF4546 0.57 -0.1
330,625 + GFF4546 0.58 +0.4
330,753 + GFF4546 0.69 -0.9
330,850 - GFF4546 0.77 -1.4
331,001 + +1.2
331,002 - -0.2
331,048 + +0.4
331,051 + +1.5
331,052 - +0.1
331,052 - -2.7
331,064 + -0.2
331,064 + -2.5
331,068 - -0.2
331,068 - -2.2
331,070 + -1.2
331,071 - +0.1

Or see this region's nucleotide sequence