Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4534 and GFF4535 are separated by 432 nucleotides GFF4535 and GFF4536 are separated by 187 nucleotides GFF4536 and GFF4537 are separated by 57 nucleotides GFF4537 and GFF4538 are separated by 150 nucleotides
GFF4534 - C4-dicarboxylate transporter DcuC (TC 2.A.61.1.1), at 320,605 to 321,948
GFF4534
GFF4535 - Lipid A acylation protein PagP, palmitoyltransferase, at 322,381 to 322,941
GFF4535
GFF4536 - Cold shock protein CspE, at 323,129 to 323,338
GFF4536
GFF4537 - CrcB protein, at 323,396 to 323,719
GFF4537
GFF4538 - Aliphatic amidase AmiE (EC 3.5.1.4), at 323,870 to 324,658
GFF4538
Position (kb) 322
323 Strain fitness (log2 ratio) -1
0
1
2 at 321.552 kb on + strand, within GFF4534 at 321.653 kb on - strand, within GFF4534 at 321.653 kb on - strand, within GFF4534 at 321.661 kb on + strand, within GFF4534 at 321.662 kb on - strand, within GFF4534 at 321.690 kb on + strand, within GFF4534 at 321.691 kb on - strand, within GFF4534 at 321.714 kb on + strand, within GFF4534 at 321.850 kb on - strand at 322.373 kb on - strand at 322.531 kb on + strand, within GFF4535 at 322.531 kb on + strand, within GFF4535 at 322.664 kb on - strand, within GFF4535 at 322.838 kb on + strand, within GFF4535 at 322.852 kb on + strand, within GFF4535 at 322.897 kb on - strand at 323.135 kb on - strand at 323.325 kb on + strand at 323.343 kb on - strand at 323.639 kb on + strand, within GFF4537 at 323.642 kb on - strand, within GFF4537 at 323.775 kb on - strand at 323.775 kb on - strand at 323.796 kb on + strand at 323.805 kb on + strand at 323.870 kb on - strand at 323.908 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 321,552 + GFF4534 0.70 +1.4 321,653 - GFF4534 0.78 -1.5 321,653 - GFF4534 0.78 +0.2 321,661 + GFF4534 0.79 +2.6 321,662 - GFF4534 0.79 -0.1 321,690 + GFF4534 0.81 -0.0 321,691 - GFF4534 0.81 +0.8 321,714 + GFF4534 0.83 +0.6 321,850 - -0.0 322,373 - -0.3 322,531 + GFF4535 0.27 -0.2 322,531 + GFF4535 0.27 -0.5 322,664 - GFF4535 0.50 -0.9 322,838 + GFF4535 0.81 -1.6 322,852 + GFF4535 0.84 -0.8 322,897 - +0.1 323,135 - +0.2 323,325 + +0.2 323,343 - +0.6 323,639 + GFF4537 0.75 +0.6 323,642 - GFF4537 0.76 -0.0 323,775 - -0.0 323,775 - -0.8 323,796 + +0.6 323,805 + +0.5 323,870 - -0.7 323,908 - +0.6
Or see this region's nucleotide sequence