Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4370 and GFF4371 are separated by 32 nucleotides GFF4371 and GFF4372 are separated by 230 nucleotides GFF4372 and GFF4373 are separated by 224 nucleotides GFF4373 and GFF4374 are separated by 11 nucleotides GFF4374 and GFF4375 are separated by 55 nucleotides
GFF4370 - Glycoprotein-polysaccharide metabolism, at 153,569 to 154,138
GFF4370
GFF4371 - methylated-DNA--protein-cysteine methyltransferase-related protein, at 154,171 to 154,560
GFF4371
GFF4372 - Uncharacterized protein ylaB, at 154,791 to 156,341
GFF4372
GFF4373 - LSU ribosomal protein L31p @ LSU ribosomal protein L31p, zinc-independent, at 156,566 to 156,826
GFF4373
GFF4374 - LSU ribosomal protein L36p, at 156,838 to 156,972
GFF4374
GFF4375 - Putative inner membrane protein, at 157,028 to 157,498
GFF4375
Position (kb) 154
155
156
157 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 153.835 kb on - strand, within GFF4370 at 153.893 kb on - strand, within GFF4370 at 153.956 kb on - strand, within GFF4370 at 153.997 kb on + strand, within GFF4370 at 154.172 kb on + strand at 154.275 kb on + strand, within GFF4371 at 154.486 kb on - strand, within GFF4371 at 154.817 kb on + strand at 154.818 kb on - strand at 154.846 kb on + strand at 154.848 kb on + strand at 154.848 kb on + strand at 154.869 kb on + strand at 154.931 kb on + strand at 154.980 kb on - strand, within GFF4372 at 155.060 kb on - strand, within GFF4372 at 155.214 kb on + strand, within GFF4372 at 155.270 kb on + strand, within GFF4372 at 155.427 kb on + strand, within GFF4372 at 155.471 kb on + strand, within GFF4372 at 155.471 kb on + strand, within GFF4372 at 155.484 kb on + strand, within GFF4372 at 155.484 kb on + strand, within GFF4372 at 155.484 kb on + strand, within GFF4372 at 155.484 kb on + strand, within GFF4372 at 155.484 kb on + strand, within GFF4372 at 155.553 kb on - strand, within GFF4372 at 155.661 kb on + strand, within GFF4372 at 155.662 kb on - strand, within GFF4372 at 155.662 kb on - strand, within GFF4372 at 155.816 kb on + strand, within GFF4372 at 155.887 kb on + strand, within GFF4372 at 155.888 kb on - strand, within GFF4372 at 156.003 kb on - strand, within GFF4372 at 156.188 kb on + strand at 156.193 kb on - strand at 156.193 kb on - strand at 156.354 kb on - strand at 156.376 kb on - strand at 156.455 kb on + strand at 156.589 kb on - strand at 156.603 kb on + strand, within GFF4373 at 156.629 kb on + strand, within GFF4373 at 156.629 kb on + strand, within GFF4373 at 156.629 kb on + strand, within GFF4373 at 156.630 kb on - strand, within GFF4373 at 156.734 kb on + strand, within GFF4373 at 156.908 kb on + strand, within GFF4374 at 157.069 kb on - strand at 157.069 kb on - strand at 157.204 kb on - strand, within GFF4375 at 157.238 kb on - strand, within GFF4375 at 157.240 kb on - strand, within GFF4375 at 157.280 kb on - strand, within GFF4375
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 153,835 - GFF4370 0.47 -0.5 153,893 - GFF4370 0.57 -0.9 153,956 - GFF4370 0.68 +0.1 153,997 + GFF4370 0.75 -1.2 154,172 + +0.7 154,275 + GFF4371 0.27 -0.2 154,486 - GFF4371 0.81 +0.0 154,817 + +0.6 154,818 - +0.6 154,846 + -0.2 154,848 + -0.4 154,848 + +0.2 154,869 + +0.1 154,931 + -1.6 154,980 - GFF4372 0.12 -0.5 155,060 - GFF4372 0.17 +0.2 155,214 + GFF4372 0.27 +0.2 155,270 + GFF4372 0.31 -0.3 155,427 + GFF4372 0.41 -0.5 155,471 + GFF4372 0.44 +1.9 155,471 + GFF4372 0.44 +0.6 155,484 + GFF4372 0.45 +0.2 155,484 + GFF4372 0.45 -0.2 155,484 + GFF4372 0.45 +0.0 155,484 + GFF4372 0.45 -1.1 155,484 + GFF4372 0.45 -0.2 155,553 - GFF4372 0.49 +4.3 155,661 + GFF4372 0.56 +0.1 155,662 - GFF4372 0.56 +0.6 155,662 - GFF4372 0.56 +1.0 155,816 + GFF4372 0.66 -0.1 155,887 + GFF4372 0.71 +0.4 155,888 - GFF4372 0.71 +2.6 156,003 - GFF4372 0.78 +2.6 156,188 + -0.6 156,193 - +3.0 156,193 - -2.5 156,354 - -1.0 156,376 - -0.6 156,455 + +0.9 156,589 - +1.4 156,603 + GFF4373 0.14 +0.4 156,629 + GFF4373 0.24 +0.9 156,629 + GFF4373 0.24 +1.8 156,629 + GFF4373 0.24 +0.6 156,630 - GFF4373 0.25 +0.6 156,734 + GFF4373 0.64 -0.1 156,908 + GFF4374 0.52 +0.1 157,069 - -0.8 157,069 - +0.5 157,204 - GFF4375 0.37 +0.4 157,238 - GFF4375 0.45 -0.2 157,240 - GFF4375 0.45 -0.2 157,280 - GFF4375 0.54 -1.1
Or see this region's nucleotide sequence