Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4333 and GFF4334 are separated by 9 nucleotides GFF4334 and GFF4335 are separated by 96 nucleotides GFF4335 and GFF4336 overlap by 38 nucleotides GFF4336 and GFF4337 are separated by 218 nucleotides
GFF4333 - 2-aminoethylphosphonate:pyruvate aminotransferase (EC 2.6.1.37), at 114,420 to 115,523
GFF4333
GFF4334 - Phosphonoacetaldehyde hydrolase (EC 3.11.1.1), at 115,533 to 116,342
GFF4334
GFF4335 - DJ-1/YajL/PfpI superfamily, includes chaperone protein YajL (former ThiJ), parkinsonism-associated protein DJ-1, peptidases PfpI, Hsp31, at 116,439 to 117,029
GFF4335
GFF4336 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 116,992 to 117,810
GFF4336
GFF4337 - FIG001943: hypothetical protein YajQ, at 118,029 to 118,520
GFF4337
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-1
0
1
2
3 at 115.459 kb on + strand at 115.459 kb on + strand at 115.459 kb on + strand at 115.459 kb on + strand at 115.460 kb on - strand at 115.509 kb on + strand at 115.521 kb on + strand at 115.553 kb on + strand at 115.718 kb on - strand, within GFF4334 at 115.788 kb on + strand, within GFF4334 at 115.841 kb on + strand, within GFF4334 at 115.895 kb on - strand, within GFF4334 at 116.262 kb on + strand at 116.306 kb on + strand at 116.449 kb on + strand at 116.450 kb on - strand at 116.454 kb on - strand at 116.454 kb on - strand at 116.575 kb on - strand, within GFF4335 at 116.679 kb on - strand, within GFF4335 at 116.679 kb on - strand, within GFF4335 at 116.994 kb on - strand at 116.994 kb on - strand at 117.117 kb on - strand, within GFF4336 at 117.216 kb on - strand, within GFF4336 at 117.303 kb on + strand, within GFF4336 at 117.304 kb on - strand, within GFF4336 at 117.427 kb on + strand, within GFF4336 at 117.489 kb on + strand, within GFF4336 at 117.489 kb on + strand, within GFF4336 at 117.490 kb on - strand, within GFF4336 at 117.495 kb on + strand, within GFF4336 at 117.496 kb on - strand, within GFF4336 at 117.496 kb on - strand, within GFF4336 at 117.526 kb on - strand, within GFF4336 at 117.553 kb on - strand, within GFF4336 at 117.580 kb on - strand, within GFF4336 at 117.612 kb on + strand, within GFF4336 at 117.704 kb on - strand, within GFF4336 at 117.798 kb on - strand at 117.826 kb on + strand at 117.826 kb on + strand at 117.950 kb on + strand at 117.991 kb on + strand at 117.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 115,459 + -1.0 115,459 + -0.4 115,459 + -0.2 115,459 + -0.7 115,460 - -0.7 115,509 + +0.1 115,521 + -0.4 115,553 + +0.3 115,718 - GFF4334 0.23 -0.3 115,788 + GFF4334 0.31 -0.8 115,841 + GFF4334 0.38 -0.6 115,895 - GFF4334 0.45 +0.1 116,262 + -0.0 116,306 + +1.4 116,449 + +1.4 116,450 - -0.4 116,454 - +0.6 116,454 - -0.6 116,575 - GFF4335 0.23 -0.4 116,679 - GFF4335 0.41 -0.4 116,679 - GFF4335 0.41 -1.1 116,994 - +0.4 116,994 - -0.4 117,117 - GFF4336 0.15 -0.3 117,216 - GFF4336 0.27 -0.8 117,303 + GFF4336 0.38 +1.6 117,304 - GFF4336 0.38 -0.6 117,427 + GFF4336 0.53 +0.6 117,489 + GFF4336 0.61 +1.4 117,489 + GFF4336 0.61 -0.6 117,490 - GFF4336 0.61 +0.6 117,495 + GFF4336 0.61 +3.3 117,496 - GFF4336 0.62 -0.2 117,496 - GFF4336 0.62 -0.7 117,526 - GFF4336 0.65 -0.5 117,553 - GFF4336 0.68 +0.2 117,580 - GFF4336 0.72 +0.9 117,612 + GFF4336 0.76 -0.6 117,704 - GFF4336 0.87 +0.6 117,798 - +2.3 117,826 + -0.4 117,826 + +0.2 117,950 + +1.4 117,991 + +0.9 117,992 - -0.3
Or see this region's nucleotide sequence