Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4278 and GFF4279 are separated by 296 nucleotides GFF4279 and GFF4280 are separated by 23 nucleotides GFF4280 and GFF4281 are separated by 166 nucleotides GFF4281 and GFF4282 are separated by 91 nucleotides
GFF4278 - Multidrug-efflux transporter, major facilitator superfamily (MFS) (TC 2.A.1), at 65,809 to 67,020
GFF4278
GFF4279 - protein iraP, at 67,317 to 67,577
GFF4279
GFF4280 - FIG01046247: hypothetical protein, at 67,601 to 67,762
GFF4280
GFF4281 - Phosphate starvation-inducible protein PsiF, at 67,929 to 68,249
GFF4281
GFF4282 - Protein YaiC, at 68,341 to 69,453
GFF4282
Position (kb) 67
68 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 66.490 kb on - strand, within GFF4278 at 66.550 kb on - strand, within GFF4278 at 66.573 kb on - strand, within GFF4278 at 66.672 kb on + strand, within GFF4278 at 66.737 kb on + strand, within GFF4278 at 66.750 kb on - strand, within GFF4278 at 66.773 kb on + strand, within GFF4278 at 66.852 kb on + strand, within GFF4278 at 66.852 kb on + strand, within GFF4278 at 66.853 kb on - strand, within GFF4278 at 66.927 kb on + strand at 66.994 kb on - strand at 67.207 kb on - strand at 67.393 kb on + strand, within GFF4279 at 67.426 kb on + strand, within GFF4279 at 67.431 kb on - strand, within GFF4279 at 67.471 kb on + strand, within GFF4279 at 67.582 kb on - strand at 67.859 kb on + strand at 67.860 kb on - strand at 67.899 kb on + strand at 67.943 kb on - strand at 68.247 kb on + strand at 68.293 kb on - strand at 68.317 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 66,490 - GFF4278 0.56 +0.1 66,550 - GFF4278 0.61 +0.2 66,573 - GFF4278 0.63 +0.1 66,672 + GFF4278 0.71 -3.2 66,737 + GFF4278 0.77 +3.1 66,750 - GFF4278 0.78 +0.1 66,773 + GFF4278 0.80 -0.4 66,852 + GFF4278 0.86 +0.3 66,852 + GFF4278 0.86 +0.2 66,853 - GFF4278 0.86 +0.9 66,927 + +2.2 66,994 - -0.7 67,207 - +0.1 67,393 + GFF4279 0.29 +0.2 67,426 + GFF4279 0.42 +0.4 67,431 - GFF4279 0.44 +1.9 67,471 + GFF4279 0.59 +0.5 67,582 - +0.7 67,859 + -1.8 67,860 - -0.3 67,899 + -0.7 67,943 - -0.2 68,247 + +1.2 68,293 - -1.3 68,317 - +0.0
Or see this region's nucleotide sequence