Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4251 and GFF4252 are separated by 188 nucleotides GFF4252 and GFF4253 are separated by 12 nucleotides
GFF4251 - Putative metabolite transport protein, at 32,101 to 33,354
GFF4251
GFF4252 - Type III restriction-modification system methylation subunit (EC 2.1.1.72), at 33,543 to 35,501
GFF4252
GFF4253 - Type III restriction-modification system StyLTI enzyme res (EC 3.1.21.5), at 35,514 to 38,483
GFF4253
Position (kb)
33
34
35
36 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 32.586 kb on + strand, within GFF4251 at 32.592 kb on - strand, within GFF4251 at 32.644 kb on + strand, within GFF4251 at 32.646 kb on + strand, within GFF4251 at 32.646 kb on + strand, within GFF4251 at 32.656 kb on - strand, within GFF4251 at 32.752 kb on + strand, within GFF4251 at 32.752 kb on + strand, within GFF4251 at 32.753 kb on - strand, within GFF4251 at 32.778 kb on + strand, within GFF4251 at 32.814 kb on + strand, within GFF4251 at 32.929 kb on - strand, within GFF4251 at 33.045 kb on + strand, within GFF4251 at 33.046 kb on - strand, within GFF4251 at 33.208 kb on + strand, within GFF4251 at 33.353 kb on - strand at 33.374 kb on + strand at 33.374 kb on + strand at 33.646 kb on - strand at 33.881 kb on + strand, within GFF4252 at 33.932 kb on + strand, within GFF4252 at 33.954 kb on + strand, within GFF4252 at 33.999 kb on + strand, within GFF4252 at 34.023 kb on - strand, within GFF4252 at 34.023 kb on - strand, within GFF4252 at 34.023 kb on - strand, within GFF4252 at 34.041 kb on - strand, within GFF4252 at 34.107 kb on + strand, within GFF4252 at 34.161 kb on - strand, within GFF4252 at 34.194 kb on - strand, within GFF4252 at 34.271 kb on + strand, within GFF4252 at 34.343 kb on - strand, within GFF4252 at 34.371 kb on - strand, within GFF4252 at 34.467 kb on + strand, within GFF4252 at 34.469 kb on + strand, within GFF4252 at 34.469 kb on + strand, within GFF4252 at 34.470 kb on - strand, within GFF4252 at 34.474 kb on - strand, within GFF4252 at 34.514 kb on - strand, within GFF4252 at 34.622 kb on + strand, within GFF4252 at 34.875 kb on - strand, within GFF4252 at 34.925 kb on + strand, within GFF4252 at 34.926 kb on - strand, within GFF4252 at 34.926 kb on - strand, within GFF4252 at 34.931 kb on + strand, within GFF4252 at 34.932 kb on - strand, within GFF4252 at 35.084 kb on + strand, within GFF4252 at 35.097 kb on - strand, within GFF4252 at 35.243 kb on + strand, within GFF4252 at 35.243 kb on + strand, within GFF4252 at 35.296 kb on - strand, within GFF4252 at 35.301 kb on + strand, within GFF4252 at 35.376 kb on - strand at 35.409 kb on + strand at 35.410 kb on - strand at 35.499 kb on + strand at 35.500 kb on - strand at 35.520 kb on - strand at 35.520 kb on - strand at 35.616 kb on + strand at 35.617 kb on - strand at 35.621 kb on + strand at 35.660 kb on + strand at 35.697 kb on + strand at 35.698 kb on - strand at 35.703 kb on + strand at 35.704 kb on - strand at 35.704 kb on - strand at 35.704 kb on - strand at 35.718 kb on + strand at 35.718 kb on + strand at 35.719 kb on - strand at 35.736 kb on + strand at 35.736 kb on + strand at 35.736 kb on + strand at 35.737 kb on - strand at 35.804 kb on + strand at 35.804 kb on + strand at 35.804 kb on + strand at 35.964 kb on - strand, within GFF4253 at 36.098 kb on + strand, within GFF4253 at 36.099 kb on - strand, within GFF4253 at 36.129 kb on - strand, within GFF4253 at 36.139 kb on - strand, within GFF4253 at 36.224 kb on + strand, within GFF4253 at 36.243 kb on + strand, within GFF4253 at 36.312 kb on - strand, within GFF4253 at 36.312 kb on - strand, within GFF4253 at 36.318 kb on - strand, within GFF4253 at 36.321 kb on + strand, within GFF4253 at 36.321 kb on + strand, within GFF4253 at 36.360 kb on + strand, within GFF4253 at 36.363 kb on - strand, within GFF4253
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 32,586 + GFF4251 0.39 +0.7 32,592 - GFF4251 0.39 -1.5 32,644 + GFF4251 0.43 +0.6 32,646 + GFF4251 0.43 +0.5 32,646 + GFF4251 0.43 +1.7 32,656 - GFF4251 0.44 -0.2 32,752 + GFF4251 0.52 -0.3 32,752 + GFF4251 0.52 -0.3 32,753 - GFF4251 0.52 -1.5 32,778 + GFF4251 0.54 +0.2 32,814 + GFF4251 0.57 +0.2 32,929 - GFF4251 0.66 -0.5 33,045 + GFF4251 0.75 -0.3 33,046 - GFF4251 0.75 -0.4 33,208 + GFF4251 0.88 -2.0 33,353 - +0.1 33,374 + +0.1 33,374 + -0.9 33,646 - +0.2 33,881 + GFF4252 0.17 -1.6 33,932 + GFF4252 0.20 +0.6 33,954 + GFF4252 0.21 -0.5 33,999 + GFF4252 0.23 +0.3 34,023 - GFF4252 0.25 -0.5 34,023 - GFF4252 0.25 -0.3 34,023 - GFF4252 0.25 -1.8 34,041 - GFF4252 0.25 -0.1 34,107 + GFF4252 0.29 -0.8 34,161 - GFF4252 0.32 -1.1 34,194 - GFF4252 0.33 -0.5 34,271 + GFF4252 0.37 -4.2 34,343 - GFF4252 0.41 -1.5 34,371 - GFF4252 0.42 -0.2 34,467 + GFF4252 0.47 -1.2 34,469 + GFF4252 0.47 -0.7 34,469 + GFF4252 0.47 -0.7 34,470 - GFF4252 0.47 -0.8 34,474 - GFF4252 0.48 -0.1 34,514 - GFF4252 0.50 -0.5 34,622 + GFF4252 0.55 -0.2 34,875 - GFF4252 0.68 -1.3 34,925 + GFF4252 0.71 -0.4 34,926 - GFF4252 0.71 -0.7 34,926 - GFF4252 0.71 -0.6 34,931 + GFF4252 0.71 -0.5 34,932 - GFF4252 0.71 +0.3 35,084 + GFF4252 0.79 -1.7 35,097 - GFF4252 0.79 -0.7 35,243 + GFF4252 0.87 -0.2 35,243 + GFF4252 0.87 -0.8 35,296 - GFF4252 0.89 -0.9 35,301 + GFF4252 0.90 -0.2 35,376 - -0.6 35,409 + -0.9 35,410 - -1.1 35,499 + +1.6 35,500 - +0.0 35,520 - +0.0 35,520 - -1.3 35,616 + +0.1 35,617 - -0.2 35,621 + -0.8 35,660 + -0.8 35,697 + -0.3 35,698 - -1.1 35,703 + -0.2 35,704 - -0.3 35,704 - -1.5 35,704 - -0.5 35,718 + -0.7 35,718 + -0.1 35,719 - -0.7 35,736 + +0.0 35,736 + +1.2 35,736 + -2.0 35,737 - -1.0 35,804 + -1.6 35,804 + -0.4 35,804 + -0.6 35,964 - GFF4253 0.15 -0.6 36,098 + GFF4253 0.20 -1.1 36,099 - GFF4253 0.20 +0.1 36,129 - GFF4253 0.21 -0.0 36,139 - GFF4253 0.21 -0.6 36,224 + GFF4253 0.24 +0.6 36,243 + GFF4253 0.25 -0.7 36,312 - GFF4253 0.27 -0.4 36,312 - GFF4253 0.27 -0.4 36,318 - GFF4253 0.27 -0.7 36,321 + GFF4253 0.27 +0.5 36,321 + GFF4253 0.27 -0.6 36,360 + GFF4253 0.28 -0.6 36,363 - GFF4253 0.29 +0.2
Or see this region's nucleotide sequence