Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3935 and GFF3936 are separated by 9 nucleotides GFF3936 and GFF3937 are separated by 3 nucleotides GFF3937 and GFF3938 overlap by 4 nucleotides GFF3938 and GFF3939 overlap by 1 nucleotides GFF3939 and GFF3940 are separated by 24 nucleotides GFF3940 and GFF3941 are separated by 18 nucleotides
GFF3935 - ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17), at 218,629 to 219,639
GFF3935
GFF3936 - Propanediol utilization polyhedral body protein PduN, at 219,649 to 219,924
GFF3936
GFF3937 - Propanediol utilization protein PduM, at 219,928 to 220,419
GFF3937
GFF3938 - Ethanolamine utilization protein similar to PduL, at 220,416 to 221,048
GFF3938
GFF3939 - Propanediol utilization polyhedral body protein PduK, at 221,048 to 221,509
GFF3939
GFF3940 - Propanediol utilization polyhedral body protein PduJ, at 221,534 to 221,809
GFF3940
GFF3941 - Propanediol dehydratase reactivation factor small subunit, at 221,828 to 222,178
GFF3941
Position (kb) 220
221
222 Strain fitness (log2 ratio) -1
0
1 at 219.627 kb on + strand at 219.663 kb on + strand at 219.667 kb on + strand at 219.875 kb on - strand, within GFF3936 at 219.940 kb on - strand at 219.940 kb on - strand at 220.628 kb on + strand, within GFF3938 at 220.628 kb on + strand, within GFF3938 at 220.629 kb on - strand, within GFF3938 at 220.959 kb on - strand, within GFF3938 at 221.724 kb on - strand, within GFF3940 at 221.829 kb on + strand at 221.958 kb on + strand, within GFF3941 at 221.958 kb on + strand, within GFF3941 at 221.959 kb on - strand, within GFF3941
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 219,627 + +0.3 219,663 + -0.9 219,667 + +0.9 219,875 - GFF3936 0.82 -1.3 219,940 - -0.8 219,940 - +0.0 220,628 + GFF3938 0.33 -1.3 220,628 + GFF3938 0.33 -0.6 220,629 - GFF3938 0.34 -0.0 220,959 - GFF3938 0.86 -1.6 221,724 - GFF3940 0.69 +1.1 221,829 + +0.4 221,958 + GFF3941 0.37 +0.3 221,958 + GFF3941 0.37 +0.7 221,959 - GFF3941 0.37 +0.2
Or see this region's nucleotide sequence