Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF390

Experiment: LB_plus_SM_buffer with S16_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF389 and GFF390 are separated by 39 nucleotidesGFF390 and GFF391 overlap by 1 nucleotides GFF389 - Oligosaccharide repeat unit polymerase Wzy; O-antigen ligase, at 103,574 to 104,788 GFF389 GFF390 - Lipopolysaccharide heptosyltransferase I (EC 2.4.1.-), at 104,828 to 105,781 GFF390 GFF391 - ADP-heptose--lipooligosaccharide heptosyltransferase II (EC 2.4.1.-), at 105,781 to 106,827 GFF391 Position (kb) 104 105 106Strain fitness (log2 ratio) -3 -2 -1 0 1at 103.882 kb on + strand, within GFF389at 104.016 kb on - strand, within GFF389at 104.144 kb on - strand, within GFF389at 104.197 kb on - strand, within GFF389at 104.947 kb on - strand, within GFF390at 104.949 kb on - strand, within GFF390at 104.956 kb on + strand, within GFF390at 105.243 kb on + strand, within GFF390at 105.322 kb on - strand, within GFF390at 105.740 kb on + strandat 105.927 kb on + strand, within GFF391at 105.957 kb on + strand, within GFF391at 106.223 kb on + strand, within GFF391at 106.686 kb on + strand, within GFF391

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI
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103,882 + GFF389 0.25 -0.6
104,016 - GFF389 0.36 -1.0
104,144 - GFF389 0.47 +0.8
104,197 - GFF389 0.51 +1.6
104,947 - GFF390 0.12 -0.2
104,949 - GFF390 0.13 +0.3
104,956 + GFF390 0.13 +0.2
105,243 + GFF390 0.44 -1.4
105,322 - GFF390 0.52 -2.9
105,740 + -0.1
105,927 + GFF391 0.14 +0.1
105,957 + GFF391 0.17 -1.8
106,223 + GFF391 0.42 -0.9
106,686 + GFF391 0.86 +1.0

Or see this region's nucleotide sequence