Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3873

Experiment: LB_plus_SM_buffer with S16_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3872 and GFF3873 are separated by 12 nucleotidesGFF3873 and GFF3874 are separated by 15 nucleotidesGFF3874 and GFF3875 are separated by 23 nucleotides GFF3872 - Colanic acid polymerase WcaD, at 148,561 to 149,775 GFF3872 GFF3873 - Colanic acid biosynthesis glycosyl transferase WcaE, at 149,788 to 150,534 GFF3873 GFF3874 - Colanic acid biosynthesis acetyltransferase WcaF (EC 2.3.1.-), at 150,550 to 151,104 GFF3874 GFF3875 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 151,128 to 152,249 GFF3875 Position (kb) 149 150 151Strain fitness (log2 ratio) -2 -1 0 1 2at 148.801 kb on + strand, within GFF3872at 149.033 kb on - strand, within GFF3872at 149.350 kb on - strand, within GFF3872at 149.457 kb on - strand, within GFF3872at 149.483 kb on - strand, within GFF3872at 150.042 kb on + strand, within GFF3873at 150.200 kb on - strand, within GFF3873at 150.200 kb on - strand, within GFF3873at 150.662 kb on + strand, within GFF3874at 150.810 kb on + strand, within GFF3874at 151.023 kb on + strand, within GFF3874at 151.265 kb on + strand, within GFF3875at 151.377 kb on + strand, within GFF3875at 151.377 kb on + strand, within GFF3875at 151.378 kb on - strand, within GFF3875at 151.378 kb on - strand, within GFF3875

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI
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148,801 + GFF3872 0.20 -0.2
149,033 - GFF3872 0.39 -0.4
149,350 - GFF3872 0.65 +0.5
149,457 - GFF3872 0.74 -0.3
149,483 - GFF3872 0.76 -0.2
150,042 + GFF3873 0.34 -1.9
150,200 - GFF3873 0.55 +0.1
150,200 - GFF3873 0.55 -0.8
150,662 + GFF3874 0.20 -1.2
150,810 + GFF3874 0.47 -1.1
151,023 + GFF3874 0.85 -0.5
151,265 + GFF3875 0.12 +2.2
151,377 + GFF3875 0.22 +0.3
151,377 + GFF3875 0.22 +0.8
151,378 - GFF3875 0.22 +1.1
151,378 - GFF3875 0.22 -0.4

Or see this region's nucleotide sequence