Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3745 and GFF3746 are separated by 401 nucleotides GFF3746 and GFF4031 are separated by 302 nucleotides GFF4031 and GFF3747 are separated by 73 nucleotides GFF3747 and GFF3748 are separated by 31 nucleotides GFF3748 and GFF3749 are separated by 179 nucleotides
GFF3745 - virulence protein, at 13,069 to 13,182
GFF3745
GFF3746 - hypothetical protein, at 13,584 to 13,718
GFF3746
GFF4031 - tRNA-Pro-GGG, at 14,021 to 14,097
GFF4031
GFF3747 - FIG001881: hydrolase of alkaline phosphatase superfamily, at 14,171 to 15,931
GFF3747
GFF3748 - FIG002927: hypothetical protein, at 15,963 to 16,190
GFF3748
GFF3749 - Nucleoid-associated protein NdpA, at 16,370 to 17,377
GFF3749
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1
2 at 13.279 kb on - strand at 13.294 kb on + strand at 13.372 kb on - strand at 13.440 kb on - strand at 13.462 kb on + strand at 13.463 kb on - strand at 13.586 kb on - strand at 13.640 kb on + strand, within GFF3746 at 13.837 kb on + strand at 13.838 kb on - strand at 13.838 kb on - strand at 14.111 kb on - strand at 14.259 kb on - strand at 14.423 kb on + strand, within GFF3747 at 14.423 kb on + strand, within GFF3747 at 14.520 kb on - strand, within GFF3747 at 14.520 kb on - strand, within GFF3747 at 14.724 kb on + strand, within GFF3747 at 14.729 kb on - strand, within GFF3747 at 14.982 kb on + strand, within GFF3747 at 14.983 kb on - strand, within GFF3747 at 14.983 kb on - strand, within GFF3747 at 15.231 kb on - strand, within GFF3747 at 16.074 kb on - strand, within GFF3748 at 16.170 kb on - strand at 16.226 kb on - strand at 16.229 kb on - strand at 16.509 kb on - strand, within GFF3749 at 16.677 kb on + strand, within GFF3749 at 16.678 kb on - strand, within GFF3749 at 16.775 kb on - strand, within GFF3749
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 13,279 - -0.7 13,294 + -1.0 13,372 - -0.4 13,440 - +0.7 13,462 + +2.3 13,463 - +0.4 13,586 - -0.2 13,640 + GFF3746 0.41 +1.3 13,837 + +0.1 13,838 - +0.9 13,838 - -1.1 14,111 - +0.3 14,259 - -0.9 14,423 + GFF3747 0.14 +1.3 14,423 + GFF3747 0.14 +0.3 14,520 - GFF3747 0.20 +1.1 14,520 - GFF3747 0.20 +0.7 14,724 + GFF3747 0.31 +0.7 14,729 - GFF3747 0.32 -1.9 14,982 + GFF3747 0.46 -0.1 14,983 - GFF3747 0.46 +1.4 14,983 - GFF3747 0.46 -0.8 15,231 - GFF3747 0.60 +1.6 16,074 - GFF3748 0.49 -0.4 16,170 - +1.0 16,226 - -0.9 16,229 - -0.8 16,509 - GFF3749 0.14 -0.0 16,677 + GFF3749 0.30 +1.5 16,678 - GFF3749 0.31 -0.8 16,775 - GFF3749 0.40 -0.9
Or see this region's nucleotide sequence