Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3664 and GFF3665 are separated by 70 nucleotides GFF3665 and GFF3666 are separated by 142 nucleotides
GFF3664 - Phosphoserine aminotransferase (EC 2.6.1.52), at 13,075 to 14,163
GFF3664
GFF3665 - 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19), at 14,234 to 15,517
GFF3665
GFF3666 - Putative metalloprotease yggG (EC 3.4.24.-), at 15,660 to 16,421
GFF3666
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1
2 at 13.507 kb on + strand, within GFF3664 at 13.508 kb on - strand, within GFF3664 at 13.516 kb on + strand, within GFF3664 at 13.652 kb on - strand, within GFF3664 at 13.718 kb on + strand, within GFF3664 at 13.757 kb on - strand, within GFF3664 at 13.757 kb on - strand, within GFF3664 at 13.903 kb on + strand, within GFF3664 at 13.903 kb on + strand, within GFF3664 at 13.917 kb on + strand, within GFF3664 at 13.917 kb on + strand, within GFF3664 at 13.917 kb on + strand, within GFF3664 at 13.996 kb on - strand, within GFF3664 at 14.049 kb on - strand, within GFF3664 at 14.090 kb on - strand at 14.162 kb on - strand at 14.165 kb on - strand at 14.423 kb on + strand, within GFF3665 at 14.572 kb on - strand, within GFF3665 at 14.572 kb on - strand, within GFF3665 at 14.878 kb on - strand, within GFF3665 at 14.924 kb on + strand, within GFF3665 at 14.942 kb on + strand, within GFF3665 at 14.943 kb on - strand, within GFF3665 at 15.149 kb on - strand, within GFF3665 at 15.289 kb on - strand, within GFF3665 at 15.329 kb on - strand, within GFF3665 at 15.335 kb on - strand, within GFF3665 at 15.392 kb on - strand at 15.451 kb on + strand at 15.451 kb on + strand at 15.479 kb on + strand at 15.582 kb on - strand at 15.676 kb on + strand at 15.676 kb on + strand at 15.679 kb on + strand at 15.769 kb on - strand, within GFF3666 at 15.812 kb on + strand, within GFF3666 at 15.870 kb on + strand, within GFF3666 at 15.871 kb on - strand, within GFF3666 at 15.936 kb on + strand, within GFF3666 at 15.936 kb on + strand, within GFF3666 at 15.948 kb on - strand, within GFF3666 at 16.294 kb on + strand, within GFF3666 at 16.389 kb on - strand at 16.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 13,507 + GFF3664 0.40 +0.0 13,508 - GFF3664 0.40 -0.4 13,516 + GFF3664 0.40 +2.1 13,652 - GFF3664 0.53 +1.6 13,718 + GFF3664 0.59 -1.6 13,757 - GFF3664 0.63 +0.5 13,757 - GFF3664 0.63 -0.3 13,903 + GFF3664 0.76 -1.4 13,903 + GFF3664 0.76 -0.4 13,917 + GFF3664 0.77 -0.6 13,917 + GFF3664 0.77 -0.7 13,917 + GFF3664 0.77 -0.5 13,996 - GFF3664 0.85 +0.2 14,049 - GFF3664 0.89 -0.4 14,090 - -1.1 14,162 - +2.1 14,165 - +0.3 14,423 + GFF3665 0.15 -1.0 14,572 - GFF3665 0.26 +0.7 14,572 - GFF3665 0.26 -1.0 14,878 - GFF3665 0.50 +0.7 14,924 + GFF3665 0.54 -0.4 14,942 + GFF3665 0.55 -1.8 14,943 - GFF3665 0.55 -0.7 15,149 - GFF3665 0.71 +0.8 15,289 - GFF3665 0.82 -0.9 15,329 - GFF3665 0.85 -1.4 15,335 - GFF3665 0.86 -1.8 15,392 - +0.1 15,451 + +1.4 15,451 + +0.8 15,479 + -0.8 15,582 - -1.3 15,676 + +0.1 15,676 + -1.4 15,679 + -0.2 15,769 - GFF3666 0.14 +0.5 15,812 + GFF3666 0.20 -0.3 15,870 + GFF3666 0.28 +0.6 15,871 - GFF3666 0.28 -1.0 15,936 + GFF3666 0.36 -0.1 15,936 + GFF3666 0.36 -0.4 15,948 - GFF3666 0.38 +0.6 16,294 + GFF3666 0.83 +0.1 16,389 - -0.5 16,476 - -0.5
Or see this region's nucleotide sequence